|Table of Contents|

Effects of cellulase on the mixed silage quality of dried maize straw and cabbage waste and an analysis of its bacterial community

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2018 05
Page:
1097-1106
Research Field:
Articles
Publishing date:

Info

Title:
Effects of cellulase on the mixed silage quality of dried maize straw and cabbage waste and an analysis of its bacterial community
Author(s):
REN Haiwei1 2 LIU Feifei1 WANG Li1 FAN Wenguang1 LI Zhizhong1** WANG Yu2 LAN Wenlin1 & LI Jinping2
1 School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China 2 Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou 730050, China
Keywords:
dry maize straw cabbage waste cellulase mixed silage quality bacterial community diversity
CLC:
X712 : Q938.1
PACS:
DOI:
10.19675/j.cnki.1006-687x.2017.12051
DocumentCode:

Abstract:
Dry maize straw (DMS) and cabbage waste (CW) were mixed and ensiled for trans-seasonal storage and examined to improve the wet storage quality of DMS, test the effects of different doses of cellulase on silage quality, and investigate the dynamics of the bacterial community within this mixed silage. This experiment used the following treatments: the control group ME (without enzyme addition), group CA (with the addition of cellulase at a low dose of 0.1%), and group CB (with the addition of cellulase at a high dose of 0.3%). All treatment groups were preserved in an airtight container for 170 day at 18 ± 1 ℃, and the chemical composition, fermentation quality, and bacterial community were observed in each group. The content of water soluble carbohydrates in groups CA and CB increased significantly compared to that in group ME. In contrast, the content of acid detergent fiber, neutral detergent fiber, crude cellulose, holocellulose, and pH in groups CA and CB decreased significantly compared to group ME during storage for 170 days. The ratio of ammonia nitrogen to total nitrogen (AN/TN) in both groups CA and CB decreased significantly between 30 and 60 days, but then underwent an increasing trend after 90 days. The ratio of lactic acid to acetic acid (LA/AA) and the ratio of lactic acid to total organic acid (LA/TOA) were both increased by the addition of cellulase, which indicated that the intensity of lactic acid fermentation was improved. During the storage process, the mixed fermentations of DMS and CW were complementary in terms of the abundance of lactic acid bacteria they stimulated, which is beneficial because it permitted the fermentation reaction to start quickly. The dominant bacteria at the phylum level were mainly Firmicutes and Proteobacteria, while the dominant bacteria at the genus level were mainly Paralactobacillus and Lactobacillus, the abundances of which were both increased by the addition of cellulose regardless of the dose used. Spoilage bacteria, such as Enterobacter, disappeared after 130 days, and the relative abundance of lactic acid bacteria in groups CA and CB always remained above 50% (except in group CA after 90 days). In conclusion, the anaerobic sealed storage of DMS and CW can help to maintain a better quality of dry straw for half a year, and the addition of cellulase improved the quality of the silage. Moreover, the low dose of cellulase (0.1% of total raw material) is recommended for use in practice considering the cost of this enzyme and its observed storage effects.

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Last Update: 2018-10-25