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[1]刘春朝,欧阳藩,郭仲琛.唐菖蒲原球茎培养的研究[J].应用与环境生物学报,1997,3(03):208-212.
 Liu Chunzhao,Ouyang Fan,Gou Zhongshen.STUDY ON PROTOCORM CULTURE OF GLADIOLUS HORTALANS[J].Chinese Journal of Applied & Environmental Biology,1997,3(03):208-212.
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唐菖蒲原球茎培养的研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
3卷
期数:
1997年03期
页码:
208-212
栏目:
论文
出版日期:
1997-08-25

文章信息/Info

Title:
STUDY ON PROTOCORM CULTURE OF GLADIOLUS HORTALANS
作者:
刘春朝1 欧阳藩1 郭仲琛2
1. 中国科学院化工冶金研究所生化工程国家重点实验室 北京 100080;
2. 中国科学院植物研究所 北京 100093
Author(s):
Liu Chunzhao Ouyang Fan Gou Zhongshen
1. State Key Lab.of Biochemical Engineering, Institute of Chemical Metallurgy, Academia Sinica, Beijing 100080;
2. Institute of Botany, Academia Sinica, Beijing 100093
关键词:
唐菖蒲原球茎组织培养双层板径向流生物反应器
Keywords:
Gladiolus hortalansprotocormtissue culturedouble tower-plate radius-flow bioreactor
摘要:
以唐菖蒲球茎芽切段为外植体在含有2,4-D的脱分化培养基上诱导的愈伤组织,可分化出具有双极性的原球茎.通过继代培养,选择了合适的培养条件:激素配比ρ(2,4-D)+ρ(NAA)=1mm/L+0.5mg/L,ρ(Sugar)=30g/L.pH5.8,θ=21~26℃.光照液体浅层培养.在优化的条件下,利用双层板径向流生物反应器进行唐菖蒲原球茎培养,28d生物量增殖8倍,原球茎数增殖9倍.
Abstract:
Protocorms of Gladiolus hortalans emerged from the segments of bud cuts when 2,4-D was added into the hasal MS medium. The influences of hormone, carbone source, temperature, illumination, pH and culture style on callus growth and protocorm formation were investigated. The proper conditions: ρ(2,4-D)=1mg/L, ρ(NAA)=0.5mg/L, ρ(Sugar)=30g/L, 0=21-26℃, pH 5.8 and shallow liquid medium illumination culture. Under the optimum conditions, a self-made double tower-plate radius-flow bioreactor was used for the protocorm culture. The results showed the protocorms grew well in the bioteactor. After the tissue was cultured for 28d, fresh weight was increased by 8 times and number of protocorms by 9 times.

参考文献/References:

1. Melra ZG. Enchanced shoot and cormlet proliferation in liquid cultured gladlolus buds by growth retardants. Plant Cell.Tismel and Organ Cullure.1989.17:101-110
2. Lillian K H. Masa propagation of new gladiolus hybrids. Acta Hort. 1987.212:631~638
3. 陆子灿.柯善强.徐立铭.唐菖蒲组织培养与人工种子的研究院;郭仲深.林濯林主编.植物体细胞胚胎发生和人工种子.北京:科学出版社.1990.106~111
4. 北京大学生物系生物化学教研室编.生物化学实验指导.北京:高等教育出版社.1988

备注/Memo

备注/Memo:
收稿日期:1996-6-19;接受日期:1996-10-19。
基金项目:国家科委基础性研究特别资助。
更新日期/Last Update: 1900-01-01