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[1]胡亚东,袁月祥,闫志英,等.一株生长pH较宽的产甲烷菌分离与系统发育分析[J].应用与环境生物学报,2009,15(04):554-558.[doi:10.3724/SP.J.1145.2009.00554]
 HU Yadong,YUAN Yuexiang,YAN Zhiying,et al.Identification and Phylogenetic Analysis of a strain Methanogen with Wide Range of Growing pH[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):554-558.[doi:10.3724/SP.J.1145.2009.00554]
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一株生长pH较宽的产甲烷菌分离与系统发育分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年04期
页码:
554-558
栏目:
生物质能源专辑
出版日期:
2009-08-25

文章信息/Info

Title:
Identification and Phylogenetic Analysis of a strain Methanogen with Wide Range of Growing pH
作者:
胡亚东袁月祥闫志英廖银章刘晓风贺蓉娜张洪森官家发
1中国科学院成都生物研究所 成都 610041
2北京合百意生态能源科技开发有限公司 北京 100073
3中国科学院研究生院 北京 100049
Author(s):
HU Yadong YUAN Yuexiang YAN Zhiying LIAO Yinzhang LIU Xiaofeng HE Rongna ZHANG Hongsen Guan Jiafa
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2Beijing Hebaiyi Ecology & Energy Technology Development Co., Ltd., Beijing 100073, China
3Graduate University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
产甲烷菌 菌种分离 厌氧发酵 生长pH 16S rDNA序列 系统发育分析
Keywords:
methanogenstrain isolationanaerobic fermentationgrowth pH16S rDNA sequencephylogenetic analysis
分类号:
Q936 : TK6
DOI:
10.3724/SP.J.1145.2009.00554
文献标志码:
A
摘要:
采用Hungate厌氧操作技术,从造纸厂阴沟污泥中分离到一株生长pH范围为5.5~9.5的产甲烷菌株SH4. 该菌对酸碱具有良好的适应性,培养3 d后,在初始pH值6.0~8.0的培养基中甲烷产量相差不大,且基本达到最大产量. SH4革兰氏染色阳性,短杆状,多数单生,不运动;菌落近圆形,微黄;利用H2+CO2或甲酸盐作为唯一碳源生长,不利用乙酸盐,对氯霉素非常敏感. 该菌最适生长pH为7.0,最适生长温度为35 ℃,最适NaCl浓度为0~1.5%. 实验表明,与仅添加厌氧污泥作为接种物相比,添加SH4菌液可使产甲烷启动时间缩短1/3,甲烷总产量亦有大幅提高. 形态、生理生化特征以及16S rDNA序列分析表明,该菌为嗜树木甲烷短杆菌(Methanobrevibacter arboriphilus). 图8 参16
Abstract:
A strain with wide growth pH range was isolated from granular sludge in a paper mill by the Hungate anaerobic techniques. The stain, named as SH4, possessed widely ranged pH from 5.5 to 9.5 for its growth and excellent ability of acclimatizing itself to acid-alkali. The methane production by this strain was found with no apparent difference if it was cultivated with different initial pH (6.0~8.0) after three days and almost equal to its maximum production. The cells of strain SH4 were G+, short rod, monothetic and nonmotile. Its colony was pale yellow with suborbicular-shape. This strain used formate or H2+CO2, but not acetate as its sole C-source, and it was very sensitive to chloramphenicol. Besides, it grew fastest at pH 7.0,55~65 ℃ and 0~2% NaCl concentration. The experiment indicated the starting time for methane production by SH4 could be shortened by one third. And the total methane yield also increased apparently. The analyses of its morphological and physicobiochemical characteristics and sequence analysis of the 16S rDNA gene of strain LG5 demonstrated that it belonged Methanobrevibacter arboriphilus. Fig 8, Ref 16

参考文献/References:

1 Sun Z (孙征), Zhou YG (周宇光), Dong XZ (东秀珠). Characterization and phylogenetics of a new species of genus methanobacterium. Acta Microbiol Sin (微生物学报), 2001, 41 (3): 265~269
2 Garcia JL, Patel B, Ollivier B. Taxonomic, phylogenetic, and ecological diversity of methanogenic Archaea. Anaerobe, 2000, 6: 205~226
3 Patel GB, Sprott GD, Fein JE. Isolation and characterization of Methanobacterium espanolae sp. nov., a mesophilic, moderately acidiphilic methanogen. Int J Syst Bacteriol, 1990, 40: 12~18
4 Liu Y, Boone DR, Choy C. Methanohalophilus oregonense sp. nov., a methylotrophic methanogen from an alkaline, saline aquifer. Int J Syst Bacteriol, 1990, 40: 111~116.
5 Mathrani IM, Boone DR, Mah RA, Fox GE, Lau PP. Methanohalophilus zhilinae sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen. Int J Syst Bacteriol, 1988, 38: 139~142
6 Kevbrin VV, Lysenko AM, Zhilina TN. Physiology of the alkaliphilic methanogen Z-7936, a new strain of Methanosalsus zhilinaeae isolated from Lake Magadi. Microbiol Engl Tr, 1997, 66: 261~266
7 Bräuer SL, Yashiro E, Ueno NG, Yavitt JB, Zinder SH. Characterization of acid-tolerant H2/CO2-utilizing methanogenic enrichment cultures from an acidic peat bog in NewYork State. FEMS Microbiol Ecol, 2006, 57: 206~216
8 钱泽澍, 闵航. 沼气发酵微生物学. 杭州: 浙江科学技术出版社, 1986. 274~275
9 Hungate RE. A roll-tube method for cultivation of strict anaerobes. Methods Microbiol, 1969, 3B: 117~132
10 Gong G (龚革), Wang XY (王修垣). Features of nine strains of thermophilic methanogens. Acta Microbiol Sin (微生物学报), 1997, 37 (5): 378~384
11 Cuzin N, Ouattara AS, Labat M, Garcia JL.Methanobacterium congolense sp nov, from a methanogenic fermentation of cassava peel. Intern J Syst & Evol Microbiol, 2001, 51 (2): 489~493
12 Wright AD, Pimm C. Improved strategy for presumptive identifieation of methanogens using 16S riboprinting. Mierobiol Methods, 2003, 55 (2): 337~349
13 农业部厌氧微生物重点开放实验室. 产甲烷菌及其研究方法. 成都: 成都电子科技大学出版社, 1997. 223~224
14 Duarte AC , Anderson GK. Inhibition modeling in anaerobic digestion. Wat Sci Technol , 1982 , 14: 749~763
15 Lin XF (凌雪峰), Zuo JE (左剑恶), Gu XS (顾夏声). Studies on cultivation of acid-tolerant methanogenic granlular sludge using river sediments. China Environ (中国环境科学), 2003, 23 (6): 587~591
16 Zuo JE (左剑恶), Lin XF (凌雪峰), Gu XS (顾夏声). A study on cultivation of acid-tolerant methanogenic granular sludge under low pH values and its characteristics. Microbiology (微生物学通报), 2004, 31 (5): 65~69

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备注/Memo

备注/Memo:
农业部“ 9 4 8”项目(No. 2 0 0 6 -Z7 ),国家科技支撑计划项目(No s .2006BAD07A02,2006BAJ04A02)和四川省科技计划项目(No. 07NG-020)资助
更新日期/Last Update: 2009-08-27