|本期目录/Table of Contents|

[1]黄搏,斡科,孙婧,等.TMV感染烟草叶片类囊体膜对热胁迫更敏感[J].应用与环境生物学报,2010,16(03):332-336.[doi:10.3724/SP.J.1145.2010.00332]
 HUANG Bo,WO Ke,SUN Jing,et al.Sensitivity of Thylakoid Membrane of TMV Infected Tobacco Leaves to Heat Stress[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):332-336.[doi:10.3724/SP.J.1145.2010.00332]
点击复制

TMV感染烟草叶片类囊体膜对热胁迫更敏感()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
332-336
栏目:
胁迫抗性生理及分子机制专栏
出版日期:
2010-06-25

文章信息/Info

Title:
Sensitivity of Thylakoid Membrane of TMV Infected Tobacco Leaves to Heat Stress
作者:
黄搏斡科孙婧万波杜林方
(1四川大学生物资源与生态环境教育部重点实验室 成都 610064)
(2中国科学院成都生物学研究所 成都 610041)
Author(s):
HUANG Bo WO Ke SUN Jing WAN Bo DU Linfang
(1Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China)
(2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
烟草花叶病毒类囊体膜热胁迫叶绿素荧光色素蛋白
Keywords:
tobacco mosaic virus thylakoid membrane heat stress chlorophyll fluorescence pigment binding protein
分类号:
Q945.78 : S572.01
DOI:
10.3724/SP.J.1145.2010.00332
文献标志码:
A
摘要:
分别利用吸收光谱、荧光发射光谱、温和电泳和SDS-PAGE的方法分析比较热胁迫(35~65 ℃)对TMV感染或未感染TMV的烟草叶片类囊体膜结构和功能的影响. 结果表明,未感染TMV的叶片类囊体膜可见光区吸收峰强度在25~45 ℃温度范围内随处理温度升高而增加,而TMV感染的几乎没有变化;未感染TMV的叶片类囊体膜叶绿素a荧光发射峰强度也随处理温度显著变化,35或45 ℃时荧光峰强度分别降低了19.5%和37%,65 ℃时减少到23.3%,而TMV感染的也显著降低. 温和电泳和SDS-PAGE结果表明,热胁迫均引起感染TMV或未感染TMV的叶片类囊体膜色素蛋白发生明显变化,如游离色素叶绿素增多,光系统Ⅱ和I复合体解聚成单体,类囊体膜上的重要功能蛋白含量减少,产生聚合物,尤其经TMV感染引发的该变化更加明显. 说明TMV感染烟草提高了类囊体膜对热胁迫的敏感
性. 图6 参20
Abstract:
With absorption spectra, fluorescence emission spectra, native electrophoresis and SDS-PAGE methods, the structure and function of thylakoid membrane under heat stress (25~65 ℃) were compared between normal or the TMV infected tobacco leaves. The results showed that the absorption peak intensity from normal leaves increased as temperature rose, while that from the infected leaves hardly changed at 25~45 ℃. The intensity of chlorophyll a emission fluorescence peak also obviously changed depending on temperature, and fluorescence peak intensity decreased by 19.5% and 37% at 35 ℃ and 45 ℃, and decreased by 23.3% at 65 ℃, while the fluorescence intensity of the infected sample also decreased notablely. The result of native electrophoresis and SDS-PAGE showed that heat stress caused the obvious change in the composition of pigment binding protein of thylakoid membrane, such as increase in free chlorophyll, formation of the aggregates, degradation of trimmer into monomer of PSⅡand PSⅠcomplex and obvious decrease in important function protein of thylakoid membrane, while the thylakoid membrane of the TMV infected leaves was obviously affected, indicating its sensitivity to heat stress by the insertion of the coat protein induced by the TMV. Fig 6, Ref 20

参考文献/References:

1 Gounaris K, Brain ARR, Quinn PJ, Williams WP. Structural reorganization of chloroplast thylakoid membranes in response to heat stress. Biochim Biophys Acta, 1984, 766: 198~208
2 Pastenes C, Horton P. Effect of high temperature on photosynthesis in beans. I. Oxygen evolution and chlorophyll fluorescence. Plant Physiol, 1996, 112: 1245~1251
3 Pospisil P, Tyystjarvi E. Molecular mechanism of high-temperature-induced inhibition of acceptor side of photosystemⅡ. Photosynth Res, 1999, 62: 55~66
4 Vani B, Pardha Saradhi P, Mohanty P. Alteration in chloroplast structure and thylakoid membrane composition due to in vivo heat treatment of rice seedlings: Correlation with the functional changes. J Plant Physiol, 2001, 158: 583~592
5 Komayama K, Khatoon M, Takenaka D, Horie J, Yamashita A, Yoshioka M, Nakayama Y, Yoshida M, Ohira S, Morita N, Velitchkova M, Enami I, Yamamoto Y. Quality control of photosystem II: Cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids. Biochim Biophys Acta, 2007, 1767: 838~846
6 Reinero A, Beachy RN. Reduced photosystem II activity and accumulation of viral coat protein in chloroplast of leaves infected with tobacco mosic virus. Plant Physiol, 1989, 89: 111~116
7 Rahoutei J, García-Luque I, Barón M. Inhibition of photosynthesis by viral infection: Effect on PS Ⅱ structure and function. Physiol Plant, 2000, 110: 286~292
8 Qin SR (秦世荣), Zhang NH (张年辉), Fu JF (付竞峰), Du LF (杜林方). Effect of TMV or ningnanmycin treatment on protein composition in leaves and thylakoid membranes of tobacco. Chin J Appl Environ Biol (应用与环境生物学报), 2004, 10 (2): 158~161
9 Du LF (杜林方), Tang XS (唐晓松), Liang HG (梁厚果). Further studies on the isolation and characterization of the oxygen evolving PSⅡ reaction center complex. Acta Photophysiol Sin (植物生理学报), 1992, 18: 17~23
10 Lichtenthaler HK, Wellburn AR. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc T, 1983, 11: 591~592
11 Fu JF (付竞峰), Qin TT(秦婷婷) , Du LF (杜林方). Effect of dark treatment on fluorescence properties of cytoledon thylakoid membranes of Brassicanapus L. Chin J Appl Environ Biol (应用与环境生物学报). 2008, 14 (1): 24~031
12 Tanaka A, Tanaka Y, Tsuji H, Resolution of chlorophyll a/b-protein complexes by polyacrylamide gel electrophoresis: Evidence for the heterogeneity of light-harvesting chlorophyll a/b-protein complexes. Plant Cell Physiol, 1987, 28: 1537~1545
13 Allen KD, Staehelin LA. Resolution of 16 to 20 chlorophyll protein comp lexes using a low ionic strength native green gel system. Anal Biochem, 1991, 194: 214~222
14 Tang YL, Wen XG, Lu QT, Yang ZP, Cheng ZK, and Lu CM, Heat stress induces an aggregation of the light-harvesting complex of photosystem Ⅱ in spinach plants. Plant Physiol, 2007, 143: 629~638
15 De Las Rivas J, Barber J. Structure and thermal stability of photosystem II reaction centers studied by infrared spectroscopy. Biochemistry, 1997, 36: 8897~8903
16 Qu YG, Chen H, Qin XC, Li LB, Wang L, Kuang TY. Thermal denaturation of CP43 studied by Fourier transform-infrared spectroscopy and terahertz time-domain spectroscopy. Biochim Biophys Acta, 2007, 1774: 1614~1618
17 Yamashita A, Nijo N, Pospí?il P, Morita N, Takenaka D, Aminaka R, Yamamoto Y, Yamamoto Y. Quality control of photosystem II: Reactive oxygen species are responsible for the damage to photosystem II under moderate heat stress. J Biol Chem, 2008, 283: 28380~28391
18 Pospí?il P, ?nyrychováI, Nau? J. Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature: EPR spin-trapping study. Biochim Biophys Acta, 2007, 1767: 854~859
19 Yoshioka M, Uchida S, Mori H, Komayama K, Ohira S, Morita N, Nakanishi T, Yamamoto Y. Quality control of photosystem II: Cleavage of reaction center D1 protein in spinach thylakoids by FtsH protease under moderate heat stress. J Biol Chem, 2006, 281: 21660~21669
20 Pérez-Bueno ML, Rahoutei J, Sajnani C, García-Luque I, Barón M. Proteomic analysis of the oxygen-evolving complex of photosystem Ⅱ under biotec stress: Studies on Nicotiana benthamiana infected with tobamoviruses. Proteomics, 2004, 4: 418~425

相似文献/References:

[1]付竞峰,秦婷婷,杜林方.暗处理对油菜子叶类囊体膜色素组成和荧光性质的影响[J].应用与环境生物学报,2008,14(01):24.
 FU Jingfeng,et al..Effect of Dark Treatment on Fluorescence Properties of Cytoledon Thylakoid Membranes of Brassica napus L.[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):24.
[2]何海燕,张丹,李斌.真菌提取物抑制烟草花叶病毒(TMV)研究进展[J].应用与环境生物学报,2016,22(01):167.[doi:10.3724/SP.J.1145.2015.07030]
 HE Haiyan,ZHANG Dan** & LI Bin.Recent progress in research on fungal extracts inhibiting tobacco mosaic virus (TMV)[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):167.[doi:10.3724/SP.J.1145.2015.07030]

备注/Memo

备注/Memo:
教育部新世纪人才支持计划(No. NCET-04-0861)和四川大学“985”项目资助 Supported by the Program for New Century Excellent Talents in Universities of China (No. NCET-04-0861) and the Grant “985” of Sichuan University of China
更新日期/Last Update: 2010-06-23