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[1]甘明哲,靳艳玲,周玲玲,等.适合鲜甘薯原料乙醇发酵的低粘度快速糖化预处理[J].应用与环境生物学报,2009,15(02):260-270.[doi:10.3724/SP.J.1145.2009.00262]
 GAN Mingzhe,JIN Yanlin,et al.Low Viscosity and Rapid Saccharification Pretreatment of Fresh Sweet Potato for Ethanol Production[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):260-270.[doi:10.3724/SP.J.1145.2009.00262]
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适合鲜甘薯原料乙醇发酵的低粘度快速糖化预处理()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年02期
页码:
260-270
栏目:
生物质能源专辑
出版日期:
2009-03-15

文章信息/Info

Title:
Low Viscosity and Rapid Saccharification Pretreatment of Fresh Sweet Potato for Ethanol Production
作者:
甘明哲靳艳玲周玲玲戚天胜赵海
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
Author(s):
GAN Mingzhe1 2 JIN Yanlin12 ZHOU Lingling1 2 QI Tiansheng1 & ZHAO Hai1**
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
鲜甘薯糖化低粘度预处理乙醇发酵
Keywords:
fresh sweet potato saccharification low viscosity pretreatment ethanol fermentation
分类号:
TQ920.6 : S531.099
DOI:
10.3724/SP.J.1145.2009.00262
文献标志码:
A
摘要:
甘薯是我国燃料乙醇生产的主要原料之一. 但由于鲜甘薯具有粘度大的特点,传统液化糖化处理很难在短时间内充分糖化原料;高粘度的醪液也难以进行管道输送,容易堵塞管路;同时,也会降低后续的乙醇发酵效率. 本文作者采用了快速粘度分析法对鲜甘薯糊化粘度特性进行了分析,进而对预处理条件进行了研究,考察了醪液的料水比、预处理温度、pH、时间、离子种类、酶类及添加方式对糖化醪液的葡萄糖值(Dextrose equivalent,DE)和粘度的影响. 获得的最佳预处理条件为:料水比2:1,126 ℃、pH 2.5条件下预处理5 min,液化,糖化时加入果胶酶40 U/g醪液, 纤维素酶0.5 U/g醪液. 糖化2 h后, 醪液DE值最高可达99.3,粘度4.5×104 mPa.s,而采用传统糖化工艺,糖化2 h后,醪液DE值仅为85.8,粘度大于1.0×105 mPa.s. 此预处理方法也可用于快速糖化不加水的醪液. 经预处理,糖化2 h,醪液DE值可达97.6,而对照仅为76.6. 后续的乙醇发酵试验表明,通过此预处理方法获得的糖化醪液对乙醇发酵无负面影响. 图1 表4 参20
Abstract:
Sweet potato is one of the major feedstock for the fuel ethanol production in China. As fresh sweet potato syrup shows high viscosity, it is hard to be fully converted to glucose by enzymes in the traditional saccharification process. The high-viscosity syrup is difficult to be transmitted in pipes, which may be easily blocked. Meanwhile it could also reduce the efficency of later ethanol fermentation. To solve these problems, the pasting property of fresh sweet potato was studied by rapid visco analysis (RVA). Effects of the pretreatment conditions including the ratio of material to water, temperature, pH, retention time, and kinds of icons and enzymes on the dextrose equivalent value (DE) and syrup viscosity were also investigated. The most favorable pretreatment conditions were the ratio of material to water 2:1, 126 ℃ for 5 min, pH 2.5. The amounts of pectinase and cellulose added in saccharification were 40 U per g syrup and 0.5U per g syrup, respectively. The highest DE of 99.3 and the lowest viscosity of 4.5×104 mPa.s were obtained by the most favorable pretreatment conditions, while those of 85.8 and over 1.0×105 mPa.s was produced by traditional treatment conditions. The pretreatment could also be applied for the material syrup without adding water, by which the DE could reach 97.6, higher than of 76.6 in normal process. The later experiments showed that it had no negative effect on the ethanol fermentation and exhibited lower viscosity. Fig 1, Tab 4, Ref 20

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备注/Memo

备注/Memo:
国家科技支撑计划项目(No. 2007BAD78B04)和四川省科技攻关项目(No. 027. X028-037)资助 Supported by the National Key Sci & Tech Project of the 11th 5-year-plan of China (No. 2007BAD78B04) and the Provincial Key Sci & Tech Project of Sichuan, China (No. 027. X028-037)
更新日期/Last Update: 2009-05-05