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[1]李思未,罗桂花,乔枫,等.白叶枯病菌PX099对CBB23抗氧化酶活性和防御基因表达的影响[J].应用与环境生物学报,2013,19(06):980-985.[doi:10.3724/SP.J.1145.2013.00980]
 LI Siwei,LUO Guihua,QIAO Feng,et al.Influence of Xanthomonas oryzae pv. oryzae PX099 Inoculation on Antioxidant Enzyme Activity and Defense Gene Expression in CBB23[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):980-985.[doi:10.3724/SP.J.1145.2013.00980]
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白叶枯病菌PX099对CBB23抗氧化酶活性和防御基因表达的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年06期
页码:
980-985
栏目:
研究论文
出版日期:
2013-12-25

文章信息/Info

Title:
Influence of Xanthomonas oryzae pv. oryzae PX099 Inoculation on Antioxidant Enzyme Activity and Defense Gene Expression in CBB23
作者:
李思未罗桂花乔枫王法军赵开军王春连
(1青海师范大学生命与地理科学学院 西宁 810008) (2中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室 北京 100081)
Author(s):
LI Siwei LUO Guihua QIAO Feng WANG Fajun ZHAO Kaijun WANG Chunlian
(1School of Life and Geographical Sciences, Qinghai Normal University, Xining 810008, China) (2Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
关键词:
水稻白叶枯病菌防御基因抗氧化酶CBB23
Keywords:
rice bacterial blight defense gene antioxidant enzyme CBB23
分类号:
S432.2 : S435.111.4+7
DOI:
10.3724/SP.J.1145.2013.00980
文献标志码:
A
摘要:
为了研究水稻抗白叶枯病材料CBB23受病原菌诱导后其叶片内与抗性相关物质发生的变化,选用抗水稻白叶枯病近等基因系CBB23及其感病轮回亲本JG30为材料,用白叶枯病菌PX099进行接种诱导,分析这2个品种接种后不同时间段叶片内抗氧化酶活性的变化,同时利用半定量RT-PCR技术分析OsPR1等防御基因的表达. 结果表明,PX099能使CBB23的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性呈先升高后降低趋势,并且增加幅度大于JG30;CBB23的丙二醛(MDA)含量增加,增加幅度低于JG30;防御反应病程相关蛋白基因(OsPR1)、几丁质酶结合蛋白基因(OsPR4)和防御反应转录因子调控基因(OsMyb)的表达增加,表达强于JG30. 总之,CBB23在PX099的诱导下抗氧化酶活性和防御基因表达均高于感病品种JG30,表明CBB23具有较大的抗病育种利用价值.

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备注/Memo

备注/Memo:
国家自然科学基金项目(31171812)资助
更新日期/Last Update: 2014-01-03