|本期目录/Table of Contents|

[1]张治国,高永峰,苗敏,等.番茄SlWD1基因的克隆及SlWD1与DDB1的相互作用[J].应用与环境生物学报,2013,19(04):623-629.[doi:10.3724/SP.J.1145.2013.00623]
 ZHANG Zhiguo,GAO Yongfeng,MIAO Min,et al.Cloning of SlWD1 Gene and Interaction of SlWD1 with DDB1 in Tomato[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):623-629.[doi:10.3724/SP.J.1145.2013.00623]
点击复制

番茄SlWD1基因的克隆及SlWD1与DDB1的相互作用()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年04期
页码:
623-629
栏目:
研究论文
出版日期:
2013-08-25

文章信息/Info

Title:
Cloning of SlWD1 Gene and Interaction of SlWD1 with DDB1 in Tomato
作者:
张治国高永峰苗敏刘继恺孙晓春张凝李頔刘永胜
(四川大学生命科学学院/水力学与山区河流开发保护国家重点实验室/生物资源与生态环境教育部重点实验室 成都 610064)
Author(s):
ZHANG Zhiguo GAO Yongfeng MIAO Min LIU Jikai SUN Xiaochun ZHANG Ning LI Di LIU Yongsheng
(College of Life Sciences, Sichuan University / State Key Laboratory of Hydraulics and Mountain River Engineering / Ministry of Education Key Laboratory for Bio-resource and Eco-environment, Chengdu 610064, China)
关键词:
番茄SlWD1基因CDT2CUL4-DDB1 E3泛素连接酶酵母双杂交免疫共沉淀
Keywords:
tomato SlWD1 gene CDT2 CUL4-DDB1 E3 ligase yeast two-hybrid assay CO-IP assays
分类号:
Q811.4 : Q78
DOI:
10.3724/SP.J.1145.2013.00623
文献标志码:
A
摘要:
在真核生物中,CDT2作为一个WD40家族蛋白,是CUL4-DDB1 E3泛素连接酶复合体的重要组成部分. 其主要功能是将CUL4-DDB1 E3泛素连接酶和靶向蛋白连接起来,从而实现靶蛋白的泛素化修饰. 利用RT-PCR技术和3’RACE技术,本研究在番茄中克隆到一个与人类CDT2同源的基因,命名为SlWD1. 通过对番茄SlWD1蛋白序列与其它模式生物的蛋白序列进行氨基酸保守结构域分析和同源性比对,发现SlWD1具有2个非常保守的WD40家族的DWD 结构域. 酵母双杂交和免疫共沉淀实验都证明,SlWD1与DDB1具有相互作用;实时定量RT-PCR分析证明,SlWD1基因在各个组织中都表达,为组成型表达,但是,在生殖器官中的表达量要明显比其它的组织高. 图7 表1 参33
Abstract:
In eukaryotes, WD40 family protein CDT2 is an important component of CUL4-DDB1 E3 ligase complex. It serves as a linker between CUL4-DDB1 and its target protein, then achieves degradation of the target protein. With 3’RACE and RT-PCR technique, we cloned a tomato gene named SlWD1, which is homologous of HsCDT2 in Homo sapiens. Amino acid sequence alignment of SlWD1/CDT2 proteins from different species showed that two conserved DWD Box domains were contained in SlWD1. Yeast two-hybrid and CO-IP assays both suggested the interaction between tomato DDB1 and SlWD1. Real-time PCR analysis indicated that SlWD1 gene expressed in all tested tissues, suggesting the expression pattern of SlWD1 was constitutive expression. However, the expression level of SlWD1 was much higher in reproductive organs than in other tissues. Fig 7, Tab 1, Ref 33

参考文献/References:

O’Connell BC, Harper JW. Ubiquitin proteasome system (UPS): what can chromatin do for you [J]? Curr Opin Cell Biol, 2007, 19 (2): 206-214
Pickart CM. Back to the future with ubiqutin [J]. Cell, 2004, 116 (2): 181-190
Petroski MD, Deshaies RJ. Function and regulation of cullin-RING ubiquitin ligases [J]. Nat Rev Mol Cell Biol, 2005, 6 (1): 9-20
Li JM, Jin J. CRL ubiquitin ligases and DNA damage response [J]. Front Oncol, 2012, 2: 29
Schwechheimer C, Villalobos LI. Cullin-containing E3 ubiquitin ligases in plant development [J]. Curr Opin Plant Biol, 2004, 7: 677-686
Jackson S, Xiong Y. CRL4s: the CUL4-RING E3 ubiquitin ligases [J]. Trends Biochem Sci, 2009, 34: 562-570
Chen X, Zhang Y, Douglas L, UV-damaged DNA-binding proteins are targets of CUL4A-mediated ubiquitination and degradation [J]. J Biol Chem, 2006, 276 (51): 48175-48182
Angers S, Li T, Yi X, Maccoss MJ, Moon RT, Zheng N. Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery [J]. Nature, 443 (7111): 590-593
He YJ, McCall CM, Hu J, Zeng Y, Xiong Y. DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases [J]. Genes Dev, 2006, 20 (21): 2949-2954
Biedermann S,Hellmann H. WD40 and CUL4-based E3ligases: lubricating all aspects of life [J]. Trends Plant Sci, 2011, 16 (1): 38-46
Kamura, T, Maenaka K, Kotoshiba S, Matsumoto M, Kohda, Conaway RC, Conaway JW, Nakayama KI. VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases [J]. Genes Dev, 18: 3055-3065
Liu C, Poitelea M, Watson A, Yoshida SH, Shimoda C, Holmberg C, Nielsen O, Carr AM. Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase [J]. EMBO J, 2005, 24: 3940-3951
Lee JH, Terzaghi W, Gusmaroli G, Charron JB, Yoon HJ, Chen H, He YJ, Xiong Y, Deng XW. Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases [J]. Plant Cell, 2008, 20 (1): 152 -167
Higa L, Banks D, Wu M, Kobayashi R, Sun H, Zhang H. L2DTL/CDT2 interacts with the CUL4/DDB1 complex and PCNA and regulates CDT1 proteolysis in response to DNA damage [J]. Cell Cycle, 2006, 5: 1675-1680
Zhang Y, Feng S, Chen F, Chen H, Wang J, McCall C, Xiong Y, Deng XW. Arabidopsis DDB1-CUL4ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes [J]. Plant Cell, 2008, 20 (6): 1437-1455
Chen H, Huang X, Gusmaroli G, Terzaghi W, Lau OS, Yanagawa Y, Zhang Y, Li J, Lee JH, Zhu D, Deng XW. Arabidopsis CULLIN4-damaged DNA binding protein 1 interacts with CONSTITUTIVELY PHOTOMORPHOGENIC1-SUPPRESSOR OF PHYA complexes to regulate photomorphogenesis and flowering time [J]. Plant Cell, 2010, 22: 108-123
Dumbliauskas E, Lechner E, Jaciubek M, Berr A, Pazhouhandeh M, Alioua M, Cognat V, Brukhin V, Koncz C, Grossniklaus U, Molinier J, Genschik P. The Arabidopsis CUL4–DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting [J]. EMBO J, 2011, 30: 731-743
Pazhouhandeh M, Molinier J, Berr A, Genschik P. MSI4/FVE interacts with CUL4-DDB1 and a PRC2-like complex to control epigenetic regulation of flowering time in Arabidopsis [J]. Proc Natl Acad Sci USA, 2011, 108: 3430-3435
Lee JH, Yoon HJ, Terzaghi W, Martinez C, Dai M, Li J, Byun MO, Deng XW. DWA1 and DWA2, two Arabidopsis DWD protein components of CUL4-based E3 ligases, act together as negative regulators in ABA signal transduction [J]. Plant Cell, 2010, 22: 1716-1732
Wang S, Liu J, Feng Y, Niu X, Giovannoni J, Liu Y. Altered plastid levels and potential for improved fruit nutrient content by downregulation of the tomato DDB1-interacting protein CUL4 [J]. Plant J, 2008, 55: 89-103
Liu Y, Roof S, Ye Z, Barry C, van Tuinen A, Vrebalov J, Bowler C, Giovannoni J. Manipulation of light signal transduction as a means of modifying fruit nutritional quality in tomato [J]. Proc Natl Acad Sci USA, 2004, 101: 9897-9902
Liu J, Tang X, Gao L, Gao Y, Li Y, Huang S, Sun X, Miao M, Zeng H, Tian X, Niu X, Zheng L, Giovannoni J, Xiao F, Liu Y. A role of tomato UV-damaged DNA binding protein 1 (DDB1) in organ size control via an epigenetic manner [J]. PLoS ONE, 2012, 7: e42621
Roodbarkelari F, Bramsiepe J, Weinl C, Marquardt S, Novák B, Jakoby MJ, Lechner E, Genschik P, Schnittger A. CULLIN4-RING FINGER-LIGASE plays a key role in the control of endoreplication cycles in Arabidopsis trichomes [J]. Proc Natl Acad Sci USA, 2010, 107: 15275-15280
Bennett EJ, Rush J, Gygi SP, Harper JW. Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics [J]. Cell, 2010, 143 (6): 951-965
刘继恺, 高永峰, 牛向丽, 刘永胜. 番茄HP1和HP2基因RNA共干涉载体的构建及遗传转化[J]. 应用与环境生物学报, 2009, 15 (5): 591-595 [Liu JK, Gao YF, Niu XL, Liu YS. Construction and transformation of co-RNAi vector of tomato HP1 and HP2 genes [J]. Chin J Appl Environ Biol, 2009, 15 (5): 591-595]
李颖楠, 冯媛媛, 刘永胜. 番茄CDT2同源基因RNA干涉载体的构建及遗传转化研究[J]. 四川大学学报(自然科学版), 2011, 48 (4): 917-923 [Li YN, Feng YY, Liu YS. Construction and transformation of RNAi vector of tomato homologue CDT2 [J]. J Sichuan Univ (Nat Sci Ed), 2011, 48 (4): 917-923]
Lieberman M, Segev O, Gilboa N, Lalazar A, Levin I. The tomato homolog of the gene encoding UV-damaged DNA binding protein 1 (DDB1) underlined as the gene that causes the high pigment-1 mutant phenotype [J]. Theor Appl Genet, 2004, 108: 1574-1581
Jin, J, Arias EE, Chen J, Harper JW, Walter JC. A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1 [J]. Mol Cell, 2004, 23: 709-721
Senga T, Sivaprasad U, Zhu W, Park JH, Arias EE, Walter JC, Dutta A. PCNA is a co-factor for Cdt1 degradationby CUL4/DDB1 mediated N-terminal ubiquitination [J]. J Biol Chem, 2006, 281: 6246-6252
Melixetian M, Ballabeni A, Masiero L, Gasparini P, Zamponi R, Bartek J, Lukas J, Helin K. Loss of Geminin induces rereplication in the presence of functional p53 [J]. J Cell Biol, 165: 473-482
Nishitani H, Shiomi Y, Iida H, Michishita M, Takami T, Tsurimoto T. CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation [J]. J Biol Chem, 2008, 283: 29045-29052
Abbas T, Shibata E, Park J, Jha S, Karnani N, Dutta A. CRL4Cdt2 regulates cell proliferation and histone gene expression by targeting PR-Set7/Set8 for degradation [J]. Mol Cell, 2010, 40: 9-21
Oda H, Hübner MR, Beck DB, Vermeulen M, Hurwitz J, Spector DL, Reinberg D. Regulation of the histone H4 monomethylase PR-Set7 by CRL4Cdt2 -mediated PCNA-dependent degradation during DNA damage [J]. Mol Cell, 2010, 12, 40 (3): 364-376

相似文献/References:

[1]葛体达,黄丹枫** 芦波 唐东梅 宋世威.无机氮和有机氮对水培番茄幼苗碳水化合物积累及氮素吸收的影响*[J].应用与环境生物学报,2008,14(05):604.
[2]张春梅,邹志荣,张志新,等.外源亚精胺对模拟干旱胁迫下番茄幼苗活性氧水平和抗氧化系统的影响[J].应用与环境生物学报,2009,15(03):301.[doi:10.3724/SP.J.1145.2009.00301]
 ZHANG Chunmei,ZOU Zhirong,ZHANG Zhixin,et al.Effects of Exogenous Spermidine on Reactive Oxygen Levels and Antioxidative System of Tomato Seedling under Polyethlene Glycol Stress[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):301.[doi:10.3724/SP.J.1145.2009.00301]
[3]刘继恺,高永峰,牛向丽,等.番茄HP1和HP2基因RNA共干涉载体的构建及遗传转化[J].应用与环境生物学报,2009,15(05):591.[doi:10.3724/SP.J.1145.2009.00591]
 LIU Jikai,GAO Yongfeng,NIU Xiangli & LIU Yongsheng.Construction and Transformation of Co-RNAi Vector of Tomato HP1 and HP2 Genes[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):591.[doi:10.3724/SP.J.1145.2009.00591]
[4]崔向超,胡君利,林先贵,等.丛枝菌根真菌与复硝酚钠在番茄育苗中的应用[J].应用与环境生物学报,2012,18(05):843.[doi:10.3724/SP.J.1145.2012.00843]
 CUI Xiangchao,HU Junli,LIN Xiangui,et al.Application of Arbuscular Mycorrhizal Fungi and Compound Sodium Nitrophenolate in Tomato Seedling Growth[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):843.[doi:10.3724/SP.J.1145.2012.00843]
[5]朱芸晔,薛冰,王安全,等.番茄bZIP转录因子家族的生物信息学分析[J].应用与环境生物学报,2014,20(05):767.[doi:10.3724/SP.J.1145.2014.01033]
 ZHU Yunye,XUE Bing,WANG Anquan,et al.Comprehensive bioinformatic analysis of bZIP transcription factors in Solanum lycopersicum[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):767.[doi:10.3724/SP.J.1145.2014.01033]
[6]张俊芳,唐晓凤,李欲翔,等.番茄SIZ1-like1基因的克隆与功能[J].应用与环境生物学报,2015,21(03):406.[doi:10.3724/SP.J.1145.2014.12016]
 ZHANG Junfang,TANG Xiaofeng,LI Yuxiang,et al.Cloning and function study of tomato SUMO E3 ligase SIZ1-like1 gene[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):406.[doi:10.3724/SP.J.1145.2014.12016]
[7]杨述章,高兰阳,孙晓春,等.过量表达SlWD6基因增强番茄抗旱和耐盐功能[J].应用与环境生物学报,2015,21(03):413.[doi:10.3724/SP.J.1145.2015.01006]
 YANG Shuzhang,GAO Lanyang,SUN Xiaochun,et al.Over-expressing SlWD6 gene to improve drought and salt tolerance of tomato[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):413.[doi:10.3724/SP.J.1145.2015.01006]
[8]郑娜,柯林峰,杨景艳,等.来源于污染土壤的植物根际细菌对番茄幼苗的促生与盐耐受机制[J].应用与环境生物学报,2018,24(01):47.[doi:10.19675/j.cnki.1006-687x.2017.03031]
 ZHENG Na,KE Linfeng,YANG Jingyan,et al.Growth improvement and salt tolerance mechanisms of tomato seedlings mediated by plant growth-promoting rhizobacteria from contaminated soils[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):47.[doi:10.19675/j.cnki.1006-687x.2017.03031]
[9]孙德智,韩晓日,彭靖,等.外源NO和水杨酸对盐胁迫下番茄幼苗光合机构的保护作用[J].应用与环境生物学报,2018,24(03):457.[doi:10.19675/j.cnki.1006-687x.2017.08019]
 SUN Dezhi**,HAN Xiaori,PENG Jing,et al.Protective effect of exogenous nitric oxide and salicylic acid on the photosynthetic apparatus of tomato seedling leaves under NaCl stress[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):457.[doi:10.19675/j.cnki.1006-687x.2017.08019]

备注/Memo

备注/Memo:
国家自然科学基金项目(31171179)和教育部博士点基金优先项目(20110181130009)资助 Supported by the National Natural Science Foundation of China (No. 31171179) and the Priority Project of Doctoral Fund of Ministry of Education of China
更新日期/Last Update: 2013-08-22