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[1]刘丛,李俭,李昌盛,等. VC、VE及Gln复合营养素对大鼠抗冷应激血清生化指标及HSP70表达量的影响[J].应用与环境生物学报,2014,20(05):887-891.[doi:10.3724/SP.J.1145.2014.01011]
 LIU Cong,LI Jian,LI Changsheng,et al. Effect of Vitamin C, Vitamin E and Glutamine compound nutrients on serum biochemical parmeters and expression of HSP70 for anti-cold stress in Wistar Rats[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):887-891.[doi:10.3724/SP.J.1145.2014.01011]
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 VC、VE及Gln复合营养素对大鼠抗冷应激血清生化指标及HSP70表达量的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
887-891
栏目:
研究论文
出版日期:
2014-10-25

文章信息/Info

Title:
 Effect of Vitamin C, Vitamin E and Glutamine compound nutrients on serum biochemical parmeters and expression of HSP70 for anti-cold stress in Wistar Rats
作者:
 刘丛 李俭 李昌盛 计红 郭景茹 李鹏 杨焕民 李士泽
 1黑龙江八一农垦大学动物科技学院 大庆 163319
2黑龙江八一农垦大学食品学院 大庆 163319
Author(s):
 LIU Cong LI Jian LI Changsheng JI Hong GUO Jingru LI Peng YANG Huanmin LI Shize
 1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
关键词:
 复合营养素抗冷应激大鼠血清生化指标HSP70
Keywords:
 compound nutrients anti-cold stress rats serum biochemical indicators HSP70
分类号:
Q494
DOI:
10.3724/SP.J.1145.2014.01011
文献标志码:
A
摘要:
为探讨日粮中联合添加维生素 C(VC)、维生素 E(VE)及谷氨酰胺(Gln)复合营养素对大鼠抗冷应激血清生化指标及HSP70表达的影响,建立了大鼠低温应激模型. 将30只清洁级雌性Wistar大鼠随机分为3组(N = 10),即冷应激对照组、冷应激+复合营养素组(VC 600 mg/kg,VE 100 mg/kg,Gln 1000 mg/kg)和常温对照组. 常温对照组在21 ℃ ± 1 ℃下饲养,冷应激+复合营养素组,灌胃复合营养素一周后突然将环境温度降至11 ℃ ± 1 ℃,应激24 h后采血. 采用Elisa法检测血清中去甲肾上腺素(NE)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、丙二醛(MDA)的含量;采用实时定量PCR方法检测外周血淋巴细胞热休克蛋白70(HSP70)mRNA的表达量. 结果显示:(1)24 h冷应激处理后,大鼠血清中NE水平明显增高,添加复合营养素对血清中NE的影响差异极显著(P < 0.01);(2)24 h冷应激处理后,大鼠血清中氧化还原指标GSH-PX、SOD、T-AOC、MDA显著下降,添加复合营养素对GSH-PX、SOD、T-AOC、MDA的影响差异极显著(P < 0.01);(3)24 h冷应激处理后,外周血淋巴细胞HSP70 mRNA的表达量有大幅度升高,添加复合营养素的试验组,淋巴细胞HSP70 mRNA的转录量显著低于冷应激对照组(P < 0.01). 本研究表明,VC、VE及Gln复合营养素可以有效提高大鼠抗氧化能力,对大鼠抵抗低温应激具有较好的保护效果.
Abstract:
This study aimed to understand the effect of VC, VE and Glutamine (Gln) addition in diet on the cold stress resistance of rats. A cold-stress model was first established on 30 clean female Wistar rats randomly divided into three groups (N = 10). The experimental group was intragastricly administered a mixture of VC, VE and Gln (VC 600 mg/kg, VE 100 mg/kg and Gln 1 000 mg/kg); the cold stress group were exposed to the temperature of 11±1 oC for 24 h after model establishment; and the normal temperature group were kept under temperature of 21±1 oC. The concentration of antioxidant parameters in serum (NE, GSH-PX, SOD, T-AOC, MDA) were detected by ELISA technology. The relative expression of HSP70 mRNA was determined by method of qRT-PCR. The results indicated that: (1) the concentration of NE in serum tended to significantly increase after 24 h of cold stress (P < 0.01); (2) the concentration of antioxidants in serum tended to significantly reduce after 24 h of cold stress (P < 0.01); (3) the HSP70 mRNA level of q-PCR test of the cold stress group was extremely significantly higher than that of the normal temperature group (P < 0.01). From the above results, it could be concluded that the compound nutrients additive of VC, VE and Gln has a good effect on anti-cold stress of Wistar rats, with the optimal dose in basal diets as VC 600 mg/kg ,VE 100 mg/kg and Gln 1 000 mg/kg.

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备注/Memo

备注/Memo:
 国家自然科学基金项目(31272524)、农业部948计划重点资助项目(2011-G35)和黑龙江省自然科学基金项目(C201103)资助 Supported by the National Natural Science Foundation of China (31272524), the 948 Key Program of the Ministry of Agriculture (2011-G35) and the Project of Natural Science Foundation of Heilongjiang Province (C201103)
更新日期/Last Update: 2014-10-30