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[1]谭泽文,郜晨,张逸凡,等.甲基营养型芽孢杆菌的分离鉴定及在防蝇产蛆环境防治中的应用[J].应用与环境生物学报,2018,24(03):631-635.[doi:10.19675/j.cnki.1006-687x.2017.02014]
 TAN Zewen,GAO Chen,ZHANG Yifan,et al.Identification of Bacillus methylotrophicus and its application in preventing fly maggot production in the environment[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):631-635.[doi:10.19675/j.cnki.1006-687x.2017.02014]
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甲基营养型芽孢杆菌的分离鉴定及在防蝇产蛆环境防治中的应用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
631-635
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
Identification of Bacillus methylotrophicus and its application in preventing fly maggot production in the environment
作者:
谭泽文郜晨张逸凡云露彭桂香谭志远
1华南农业大学农学院 广州 510642 2华南农业大学资源环境学院 广州 510642
Author(s):
TAN Zewen1 GAO Chen1 ZHANG Yifan2 YUN Lu2 PENG Guixiang2 & TAN Zhiyuan1**
1 College of Agriculture, South China Agricultural University, Guangzhou 510642, China 2 College of Resource Environment, South China Agricultural University, Guangzhou 510642, China
关键词:
甲基营养型芽孢杆菌餐厨垃圾防蝇产蛆生物特性
Keywords:
Bacillus methylotrophicus kitchen waste prevention of fly maggot production biological property
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2017.02014
摘要:
从华南农业大学试验基地土壤筛选芽孢杆菌,得到17株芽孢杆菌,结合插入序列指纹图谱对分离获得的芽孢杆菌进行聚类分析,共分为3个类群;测定各类群代表菌株的16S rDNA序列,分析其系统发育,结果表明类群I代表菌株RF2与甲基营养型芽孢杆菌(Bacillus methylotrophicus CBMB 205T)相似性为99.48%;类群II代表菌株RF10与好氧芽孢杆菌(Bacillus aerophilus 28KT)相似性为99.61%;类群III代表菌株RF16与蜡样芽孢杆菌(Bacillus cereus ATCC 14579T)相似性为99.87%. 餐厨垃圾试验表明类群I RF2具有餐厨垃圾防蝇产蛆效果;并且生理生化、碳源利用以及抗菌谱试验表明菌株RF2在碱性条件下可以将葡萄糖分解成丙酮酸并脱羧氧化生成二乙酰,既能够将氨转化为氨基酸和胞内其他含氮化合物,也利用多种碳源供自身生长,能够拮抗多种植物病原菌(水稻纹枯病、尖刀廉孢菌、香蕉枯萎病). 而RF2在含有难溶性的磷酸盐[Ca3(PO4)2]条件下可分泌出有机酸降低pH值,使难溶性磷酸盐溶解供自身利用. 因此本研究获得的菌株RF2是一株具有较强促生作用和应用于防蝇产蛆的芽孢杆菌,具有很好的应用前景. (图4 表1 参31)
Abstract:
This research aimed to screen Bacillus spp. to prevent the production of fly maggot on kitchen wastes from soil in the experimental bases of the South China Agricultural University. A nutrient-rich medium was used to isolate the Bacillus spp. with high temperature treatment. The seventeen Bacillus strains were obtained and assigned to three groups by using Insertion Sequence based PCR (IS-PCR) DNA fingerprinting patterns. The homology of the 16S rDNA gene was 100% between Group I and Bacillus methylotrophicus CBMB 205T, 99.61% between Group II and Bacillus aerophilus 28KT, and 99.87% between Group III and Bacillus cereus ATCC 14579T. The results of kitchen waste tests proved that the representative bacteria of Group I RF2 had the ability to prevent fly maggot production on kitchen wastes. Furthermore, the results of physiological and biochemical tests, carbon utilization tests, and antagonists against plant pathogen tests showed that the bacteria from Group I RF2 had the ability to decompose glucose into pyruvate and then decarboxylate pyruvate to diacetyl under alkaline conditions, convert ammonia to intracellular amino acids or other kinds of nitrogenous compounds, use many kinds of carbon source for self-growth, and be antagonistic against plant pathogenic rice sheath blight disease, banana?fusarium?wilt, and Fusarium?oxysporum. Besides, the bacteria from RF2 could secret organic acids to dissolve insoluble phosphate [Ca3(PO4)2] for its own growth by decreasing the pH. Group I RF2 was a strong plant-promotion bacterium, and had good prospects of application for preventing fly maggot production on kitchen wastes.

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更新日期/Last Update: 2018-06-30