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[1]甘国娟,田红灯,潘永龙,等.一株新的高效偶氮染料脱色菌Paenibacillus dendritiformis GGJ7及其脱色作用[J].应用与环境生物学报,2018,24(03):563-569.[doi:10.19675/j.cnki.1006-687x.2017.03045]
 GAN Guojuan,,et al.A new efficient azo dye-decolorizing bacterium Paenibacillus dendritiformis GGJ7 and its decolorization process[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):563-569.[doi:10.19675/j.cnki.1006-687x.2017.03045]
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一株新的高效偶氮染料脱色菌Paenibacillus dendritiformis GGJ7及其脱色作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
563-569
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
A new efficient azo dye-decolorizing bacterium Paenibacillus dendritiformis GGJ7 and its decolorization process
作者:
甘国娟田红灯潘永龙Asit Mazumder潘响亮梁岩
1广西师范学院环境与生命科学学院 南宁 530001 2中国科学院深圳先进技术研究院食品安全与环境技术研究室 深圳 518055 3中国科学院新疆生态与地理研究所 乌鲁木齐 830011 4广西壮族自治区林业科学研究院 南宁 530002
Author(s):
GAN Guojuan1 2 3 TIAN Hongdeng4 PAN Yonglong2 Asit Mazumder2 PAN Xiangliang3 LIANG Yan2 3**
1 School of Environmental and Life Sciences, Guangxi Teachers Education University, Nanning 530001, China 2 Laboratory for Food Safety and Environmental Technology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China 3 Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China 4 Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning 530002, China
关键词:
刚果红Paenibacillus dendritiformis偶氮染料脱色
Keywords:
Congo Red Paenibacillus dendritiformis azo dye decolorization
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2017.03045
摘要:
从刚果红染料中分离到一株刚果红高效脱色菌,经16S rRNA 基因序列(NCBI accession No. KY655213)鉴定,该菌株属于类芽孢杆菌(Paenibacillus),将该菌株命名为Paenibacillus dendritiformis GGJ7(简写为GGJ7). 将其运用于偶氮染料脱色,并研究不同营养条件、不同培养条件(温度、pH、溶解氧)和不同染料下的脱色性能. 结果表明,GGJ7对刚果红脱色效果远高于以前工作中分离到的YRJ1、YRJ2等8株同样具有脱色功能的细菌;该菌株脱色的最佳条件为30 ℃、pH 7、25 g/L LB培养基厌氧培养;其脱色机理以生物降解为主,且脱色过程符合一级反应动力学方程:-ln (At /A0) = 0.6058t - 0.1082. 经测试,GGJ7对多种偶氮染料的脱色率高达95%,其中50 mg/L甲基橙、25 mg/L藏花猩红和25 mg/L甲基红仅需1 h,50 mg/L橙黄G和50 mg/L橙黄G6仅需3 h,50 mg/L刚果红仅需4 h. 可见GGJ7是一株高效偶氮染料脱色菌,具有处理印染废水的开发应用潜能. (图8 表2 参35)
Abstract:
This paper aimed to find an efficient bacterium for decolorizing azo dyes. A strain which could decolorize Congo Red efficiently was isolated from Congo Red. The strain was identified as Paenibacillus dendritiformis GGJ7 (GGJ7, in short) by 16S rRNA gene sequence (NCBI accession No. KY655213). Strain GGJ7 was applied to the decolorization of azo dyes in this research, and influencing factors of decolorization were investigated, including diverse nutritional conditions, culture conditions (pH, temperature, oxygen conditions), and various dyes. The results demonstrated that the decolorization rate of Congo Red by strain GGJ7 was much higher than that of the other eight strains (e.g., YRJ1, YRJ2 etc.) in our previous work. The optimal conditions for Congo Red decolorization were 25 g/L LB broth as nutrient source, 30 ℃, pH 7, and an anaerobic environment. The mechanism of decolorization was mainly biodegradation, and the decolorization process of strain GGJ7 was conformed to the first-class kinetics model: -ln (At /A0) = 0.6058t - 0.1082. For different azo dyes, the decolorization rate was up to 95%. Strain GGJ7 only needed 1 h to decolorize 50 mg/L Methyl Orange, 25 mg/L Croceine Scarlet, and 25 mg/L Methyl Red, needed 3 h to decolorize 50 mg/L Orange G and 50 mg/L Orange G6, and needed 4 h to decolorize 50 mg/L Congo Red. In summary, strain GGJ7 is an efficient azo dye-decolorizing bacterium, and it has a potential application in treating printing and dyeing wastewater.

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更新日期/Last Update: 2018-06-30