|本期目录/Table of Contents|

[1]任海伟,王聪,马延琴,等.接种不同乳酸菌对干玉米秸秆与白菜废弃物混贮品质的影响[J].应用与环境生物学报,2018,24(03):547-556.[doi:10.19675/j.cnki.1006-687x.2017.06033]
 REN Haiwei,WANG Cong,et al.Effects of inoculating different lactic acid bacteria on the mixed storage quality of dry maize straw and cabbage waste[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):547-556.[doi:10.19675/j.cnki.1006-687x.2017.06033]
点击复制

接种不同乳酸菌对干玉米秸秆与白菜废弃物混贮品质的影响()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
547-556
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
Effects of inoculating different lactic acid bacteria on the mixed storage quality of dry maize straw and cabbage waste
作者:
任海伟王聪马延琴李志忠范文广王昱张丙云秦州州余倩倩
1兰州理工大学生命科学与工程学院 兰州 730050 2甘肃省生物质能与太阳能互补供能系统重点实验室 兰州 730050 3华南农业大学园艺学院 广州 510642
Author(s):
REN Haiwei1 2 WANG Cong1 MA Yanqin1 LI Zhizhong1** FAN Wenguang1 WANG Yu2 ZHANG Bingyun1 QIN Zhouzhou1 & YU Qianqian3
1 School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China 2 Key Laboratory of Complementary Energy System of Biomass and Solar Energy of Gansu, Lanzhou 730050, China 3 Horticultural Institute, South China Agricultural University, Guangzhou 510642, China
关键词:
植物乳杆菌布氏乳杆菌有机化学组分发酵品质微生物多样性
Keywords:
Lactobacillus plantarum Lactobacillus buchneri organic chemical composition fermentation quality microbial community diversity
分类号:
X712 : Q920
DOI:
10.19675/j.cnki.1006-687x.2017.06033
摘要:
动态研究植物乳杆菌(Lactobacillus plantarum,LP)和布氏乳杆菌(Lactoacillus buchneri,LB)对干玉米秸秆与白菜废弃物连续混贮170 d期间品质变化的影响,利用MiSeq高通量测序平台考察细菌多样性. 无添加剂设为对照组(ME组),定期分析感官品质、有机化学组分、发酵品质和细菌多样性. 结果表明,与ME组相比,30 d时LB和LP组干物质(DM)含量均显著(P < 0.05)下降,之后分别于60 d和90 d显著(P < 0.05)增加,且LB组始终高于LP组. 贮存170 d期间LP和LB组中可溶性碳水化合物(WSC)含量显著(P < 0.05)高于ME组,且LB组始终高于LP组(除130 d). 30-90 d期间LP和LB组pH显著(P < 0.05)低于ME组,且LP组始终低于LB组,LP组乳酸(LA)含量显著(P < 0.05)增加,氨态氮/总氮(AN/TN)显著(P < 0.05)降低. 130-170 d期间LP和LB组中乳酸/乙酸(LA/AA)和乳酸/总有机酸(LA/TOA)均明显低于ME组,乳酸发酵程度变弱. 3个处理组中厚壁菌(Firmicutes)和变形菌(Proteobacteria)为门水平优势类群;乳杆菌(Lactobacillus)始终为属水平优势菌;2种乳酸菌剂均能使肠杆菌(Enterobacter)、假单胞菌(Pseudomonas)和黄杆菌(Flavobacterium)等腐败菌丰度显著(P < 0.05)降低,130 d时Enterobacter消失. 总之,L. plantarum有助于改善混贮中前期(90 d)发酵品质,能有效保存纤维素、半纤维素和综纤维素等能源组分,L. buchneri有利于170 d贮存期间的DM和WSC保存;建议实际生产中根据贮存周期长短来选择适宜的乳酸菌,以便获得最佳贮存品质. (图3 表5 参36)
Abstract:
The effects of two microbial inoculants, Lactobacillus plantarum (LP) and Lactobacillus buchneri (LB) on the mixed storage quality of dry maize straw and cabbage waste for 170 days were studied dynamically, and the dynamic rule of microbial community diversity were also investigated by Illumina MiSeq high-throughput sequencing. The control group (ME group) without additives was tested, and the sensory quality, organic chemical composition, fermentation quality, and microbial community diversity were analyzed regularly. The contents of dry matter (DM) in case of LP and LB decreased significantly (P < 0.05) after 30 d, but the content of DM in case of LP and LB were both significantly (P < 0.05) higher than that of the ME group after 60 d and 90 d, respectively. The content of DM was always higher in case of LB than in case of LP. The water soluble carbohydrate (WSC) content in case of LP and LB were significantly (P < 0.05) higher than that of the ME group after 170 days, and the WSC content in case of LB were higher than that in case of LP (except after 130 d). The pH values in case of LP and LB were significantly (P < 0.05) lower than that of the ME group between 30 d and 90 d, and the pH in case of LP was always lower than that of LB. The contents of lactic acid (LA) in case of LP increased significantly (P < 0.05), and the ratio of ammonia nitrogen to total nitrogen (AN/TN) decreased significantly (P < 0.05) compared to the ME group. The ratio of lactic acid to acetic acid (LA/AA) and the ratio of lactic acid to total organic acid (LA/TOA) were significantly (P < 0.05) lower than those in the ME group, and the intensity of lactic acid fermentation was weakened during 130–170 d. The dominant bacterial communities in the three groups were Firmicutes and Proteobacteria at the phylum level, while Lactobacillus was dominant in genus level. The abundance of spoilage bacteria such as Enterobacter, Pseudomonas, and Flavobacterium decreased significantly (P < 0.05) after inoculation of LP and LB, and Enterobacter disappeared after storage for 130 d. In conclusion, the fermentation quality was improved by the addition of LP after 90 d, and the energy components of cellulose, hemicellulose, and holocellulose were well preserved. The addition of LB facilitates the preservation of DM and WSCs for 170 d. It is suggested that suitable lactic acid bacteria should be selected according to the storage period in order to obtain the best storage quality.

参考文献/References:

1. 任海伟, 赵拓, 李金平, 李雪雁, 李志忠, 徐娜, 王永刚, 喻春来, 高晓航, 王晓力. 玉米秸秆与废弃白菜混贮料的发酵特性及其乳酸菌分离鉴定[J]. 草业科学, 2015, 32 (9): 1508-1517 [Ren HW, Zhao T, Li JP, Li XY, Li ZZ, Xu N, Wang YG, Yu CL, Gao XH, Wang XL. Identification of lactic acid bacteria and fermentation characteristics of mixed ensilages of corn stover and cabbage waste [J]. Pratacult Sci, 2015, 32 (9): 1508-1517]
2. 杨道兰, 汪建旭, 冯炜弘, 张艳, 尹燕, 王永林. 花椰菜茎叶与玉米秸秆的混贮品质[J]. 草业科学, 2014, 31 (3): 551-557 [Yang DL, Wang JX, Feng WH , Zhang Y, Yin Y, Wang YL. Effects of broccoli stems and leaves and maize straw mix-ensiling on silage quality [J]. Pratacult Sci, 2014, 31 (3): 551-557]
3. 任海伟, 王聪, 窦俊伟, 李志忠, 李金平, 孙永明. 玉米秸秆与废弃白菜的混合青贮品质及产沼气能力分析[J]. 农业工程学报, 2016, 32 (12): 187-194 [Ren HW, Wang C, Dou JY, Li ZZ, Li JP, Sun YM. Mixed ensiling quality of maize straw with waste cabbage and biogas production potential analysis [J]. Trans Chin Soc Agric Eng, 2016, 32 (12): 187-194]
4. 冯炜弘, 汪建旭, 杨道兰, 张艳, 尹燕, 王永林, 魏万爱. 乳酸菌剂对花椰菜茎叶青贮饲料发酵品质的影响[J]. 中国饲料, 2013 (15): 19-24 [Feng WH, Wang JX, Yang DL, Zhang Y, Yin Y, Wang YL, Wei WA. Effects of lactic acid bacteria on the fermentation quality of broccoli stalk and leaf silage [J]. China Feed, 2013 (15): 19-24]
5. Aksu T, Baytok E D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility [J]. Small Rumin Res, 2004, 55 (3): 249-252
6. Li J, Yang CH, Jia LJ, Chen LZ, Li PF. Ensiling whipgrass in large containers with Lactobacilus plantarum and Lactobacillus buchneri [J]. Anim Husb Feed Sci, 2013 (4): 169-171
7. Yuan XJ, Guo G, Wen AY, Seare T Desta, Wang J, Wang Y, Shao T. The effect of different additives on the fermentation quality, in vitro, digestibility and aerobic stability of a total mixed ration silage [J]. Anim Feed Sci Technol, 2015, 207: 41-50
8. Liu QH, Li XY, Seare T Desta, Zhang JG, Shao T. Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw [J]. J Integrat Agric, 2016, 15 (9): 2087- 2096
9. 薛艳林, 孙林, 殷国梅, 张园园, 白春生, 孙娟娟, 门果桃, 赵和平, 玉柱, 孙启忠, 张宇, 刘思博. 生物添加剂和填装密度对菊芋青贮饲料品质的影响[J]. 畜牧与饲料科学, 2017, 38 (1): 39-43 [Xue YL, Sun L, Yin GM, Zhang YY, Bai CS, Sun JJ, Men GT, Zhao HP, Yu Z, Sun QZ, Zhang Y, Liu SB. Effects of bio-additives and packing density on fermentation quality of Helianthus tuberosus L. silage [J]. Anim Husb & Feed Sci, 2017, 38 (1): 39-43]
10. 卫莹莹, 玉柱. 不同添加剂对高丹草青贮的影响[J]. 草地学报, 2016, 24 (3): 658-662 [Wei Y Y, Yu Z. Effects of different additives on the silage quality of sorghum-sudangrass hybrid [J]. Acta Agrestia Sin, 2016, 24 (3): 658-662]
11. 关皓, 郭旭生, 干友民, 白慧娟, 宋珊. 添加剂对不同含水量多花黑麦草青贮发酵品质及有氧稳定性的影响[J]. 草地学报, 2016, 24 (3): 669-675 [Guan H, Guo XS, Gan YM, Bai HJ, Song S. Effects of different additives on fermentation quality and aerobic stability of Italian ryegrass silage ensiled at different moisture [J]. Acta Agrestia Sin, 2016, 24 (3): 669-675]
12. Piotr OP, Anne BT, Jens ES. Ensiling: Wet-storage method for lignocellulosic biomass for bioethanol production [J]. Biom Bioeng, 2011, 35: 2087-2092
13. Li LH, Sun YM, Yuan ZH, Kong XY, Wao Y, Yang LG, Zhang Y, Li D. Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of Manyflower silvergrass [J]. Bioresour Technol, 2015, 189 (10): 334-340
14. 陶莲, 刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报, 2016, 28 (1): 198-207 [Tao L, Diao Q Y. Effects of ensiling on quality and bacteria composition of corn stalk [J]. Chin J Anim Nutr, 2016, 28 (1): 198-207]
15. 许颖, 马德胜, 宋文枫, 魏小芳. 16S rDNA高通量测序技术分析油藏微生物多样性[J]. 应用与环境生物学报, 2016, 22 (3): 409-414 [Xu Y, Ma DS, Song WF, Wei XF. 16S rDNA-assisted high-throughput sequencing analysis of microbial diversity in oil reservoirs [J]. Chin J Appl Environ Biol, 2016, 22 (3): 409-414]
16. 冯骁骋, 曾洁, 李伟军, 高静, 王志军. 天然草原牧草青贮饲料评级体系进展研究[J]. 草原与草业, 2016, 28 (1): 48-52 [Feng XC, Zeng J, Li WJ, Gao J, Wang ZJ. Study on the grading system of natural grassland forage silage [J]. Grassl Pratacult, 2016, 28 (1): 48-52]
17. Ren HW, Na X, Li JP, Li ZZ, Zhao T, Pei FX, Yao XQ, Sun YM. Effects of different mixed ratio of maize straw and cabbage wastes on silage quality [J]. J Biobas Mater Bioeng, 2015, 9 (1): 88-94
18. Kung L Jr, Ranjit N K. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage [J]. J Dairy Sci, 2001, 84 (5): 1149-1155
19. Zheng Y, Yu CW, Cheng YS, Zhang RH, Jenkins B, VanderGheynst JS. Effects of ensilage on storage and enzymatic degradability of sugar beet pulp [J]. Bioresour Technol, 2011, 102 (2): 1489-1495
20. 张宁, 张永根, 李欣新, 王亮. 添加2种乳酸菌制剂对水稻秸青贮品质及有氧稳定性的影响[J]. 中国畜牧杂志, 2013, 49 (5): 72-75 [Zhang N, Zhang YG, Li XX, Wang L. Effects of two kinds of lactic acid bacteria on the quality and aerobic stability of rice straw silage [J]. Chin J Anim Husb, 2013, 49 (5): 72-75]
21. 万学瑞, 吴建平, 雷赵民, 何轶群, 吴润. 优良抑菌活性乳酸菌对玉米青贮及有氧暴露期微生物数量和pH的影响[J]. 草业学报, 2016, 25 (4): 204-211 [Wan XR, Wu JP, Lei ZM, He YQ, Wu R. Effects of lactic acid bacteria on corn silage quality and stability after aerobic exposure [J]. Acta Pratacult Sin, 2016, 25 (4): 204-211]
22. Lima R, Díaz R F, Castro A, Hoedtke S, Fievez V. Multifactorial models to assess responses to sorghum proportion, molasses and bacterial inoculant on in vitro quality of sorghum-soybean silages [J]. Anim Feed Sci Technol, 2011, 164 (3): 161-173
23. 任海伟, 窦俊伟, 赵拓, 李雪雁, 李志忠, 李金平, 孙文斌, 黄娟娟. 添加剂对玉米秸秆和莴笋叶混贮品质的影响[J]. 草业学报, 2016, 25 (10): 142-152 [Ren HW, Dou JW, Zhao T, Li XY, Li ZZ, Li JP, Sun WB, Huang JJ. Effects of additives on the mixed silage quality of corn stover and asparagus lettuce leaves [J]. Acta Pratacult Sin, 2016, 25 (10): 142-152]
24. Chmidt RJ, Jr KL. The effects of Lactobacillus buchneri with or without a homolactic bacterium on the fermentation and aerobic stability of corn silages made at different locations [J]. J Dairy Sci, 2010, 93 (4): 1616-1624
25. Filya I, Sucu E. The effect of bacterial inoculants and a chemical preservative on the fermentation and aerobic stability of whole-crop cereal silages [J]. Asian Australas J Anim Sci, 2007, 68 (20): 499-518
26. Weinberg ZG, Ashbell G, Hen Y, Azrieli A, Szakacs G, Filya I. Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri [J]. J Ind Microbiol Biotechnol, 2002, 28 (1): 7-11
27. 侯建建, 白春生, 张庆, 玉柱. 单一和复合乳酸菌添加水平对苜蓿青贮营养品质及蛋白组分的影响[J]. 草业科学, 2016, 33 (10): 2119-2125 [Hou JJ, Bai CS, Zhang Q, Yu Z. Effects of different additives amount of single and multiple lactic acid bacteria on the silage quality and protein fractions of alfalfa [J]. Pratacult Sci, 2016, 33 (10): 2119-2125]
28. 孟庆翔, 杨军香. 全株玉米青贮制作与质量评价[M]. 北京: 中国农业科学技术出版社, 2016 [Meng QX, Yang JX. Production and Quality Evaluation of Whole Plant Corn Silage [M]. Beijing: China Agricultural Science and Technology Press, 2016]
29. Muck RE, Kung L Jr. Effects of Silage Additives on Ensiling in Silage: Field to Feed Bunk [M]. Ithaca: Northeast Regional Agricultural Engineering Service, 1997: 187-199
30. 董志浩, 原现军, 闻爱友, 王坚, 郭刚, 李君风, 白晰, 周顺陶, 邵涛. 添加乳酸菌和发酵底物对桑叶青贮发酵品质的影响[J]. 草业学报, 2016, 25 (6): 167-174 [Dong ZH, Y XJ, Wen AY, Wang J, Guo G, Li JF, Bai X, Zhou ST, Shao T. Effects of lactic acid bacteria and fermentation substrates on the quality of mulberry leaf silage [J]. Acta Pratacult Sin, 2016, 25 (6): 167-174]
31. Li XX, Xu WB, Yang JS, Zhao HB, Pan CF, Ding X, Zhang YG. Effects of applying lactic acid bacteria to the fermentation on a mixture of corn steep liquor and air-dried rice straw [J]. Anim Nutr, 2016, 2 (3): 229-233
32. 贾春旺, 原现军, 肖慎华, 李君风, 白晰, 闻爱友, 郭刚, 邵涛. 青稞秸秆替代苇状羊茅对全混合日粮青贮早期发酵品质及有氧稳定性的影响[J]. 草业学报 2016, 25 (4): 179-187 [Jia CW, Yuan XJ, Xiao SH, Li JF, Bai X, Wen AY, Guo G, Shao T. Effect of substituting hulless barley straw for tall fescue on early fermentation quality and aerobic stability of mixed-ration silage in Tibet [J]. Acta Pratacul Sin, 2016, 25 (4): 179-187]
33. Ennahar S, Cai Y, Fujita Y. Traffic management system and method for multi-carrier CDMA wireless networks [J]. Appl Environ Microbiol, 2003, 69 (1): 69-74
34. 郝薇. TMR发酵过程中微生物及其蛋白酶对蛋白降解的作用机理研究[D]. 北京: 中国农业大学, 2015 [Hao W. Mechanism of protein degradation by microorganisms and proteinases during fermentation of total mixed ration [D]. Beijing: China Agricultural University, 2015]
35. Muck RE. Silage microbiology and its control through additives [J]. Rev Brasil De Zoot, 2010, 39 (39): 183-191
36. 买尔哈巴?艾合买提, 樊振, 李越中, 古丽斯玛依?艾拜都拉, 乌斯满?依米提. 瘤胃中纤维素分解菌的分离、鉴定及其产酶条件的优化[J]. 微生物学报, 2013, 53 (5): 470-477 [Akomat BH, Fan Z, Li YZ, Abdura G, Imite O. Isolation and identification of rumen bacteria for cellulolytic enzyme production [J]. Acta Microbiol Sin, 2013, 53 (5): 470-477]
37.

相似文献/References:

[1]郭洪秀,张白梅,冯顺利,等.一株丁二酮高产菌株的鉴定及发酵条件优化[J].应用与环境生物学报,2015,21(01):41.[doi:10.3724/SP.J.1145.2014.03032]
 GUO Hongxiu,ZHANG Baimei,FENG Shunli,et al.Identification and fermentation optimization of a high butanedione yielding strain[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):41.[doi:10.3724/SP.J.1145.2014.03032]
[2]张白梅,徐辉,曹瑜,等.紫外诱变选育高产丁二酮乳酸菌株及其低成本发酵优化[J].应用与环境生物学报,2017,23(03):437.[doi:2015.05032]
 ZHANG Baimei,XU Hui,CAO Yu,et al.Ultraviolet mutagenic selection of a lactobacillus with high productivity of butanedione and low cost optimization of fermentation[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):437.[doi:2015.05032]

更新日期/Last Update: 2018-06-30