|本期目录/Table of Contents|

[1]刘其根,李丰,罗衡,等.引入养殖鳖对稻田土壤固氮菌的影响[J].应用与环境生物学报,2018,24(03):483-492.[doi:10.19675/j.cnki.1006-687x.2017.06041]
 LIU Qigen,,et al.The influence of introducing soft-shelled turtle on soil nitrogen-fixing microorganisms in paddy field[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):483-492.[doi:10.19675/j.cnki.1006-687x.2017.06041]
点击复制

引入养殖鳖对稻田土壤固氮菌的影响()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
483-492
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
The influence of introducing soft-shelled turtle on soil nitrogen-fixing microorganisms in paddy field
作者:
刘其根李丰罗衡郭海松张文博
1水产科学国家级实验教学示范中心(上海海洋大学) 上海 201306 2上海水产养殖工程技术研究中心 (上海海洋大学) 上海 201306 3农业部鱼类营养与环境生态研究中心(上海海洋大学) 上海 201306
Author(s):
LIU Qigen1 2 3 LI Feng1 2 3 LUO Heng1 2 3 GUO Haisong1 2 3 & ZHANG Wenbo1 2 3**
1 National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China 2 Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China 3 Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China
关键词:
稻鳖共作稻田单作Illumina高通量测序nifH基因固氮菌
Keywords:
rice turtle co-culture rice monoculture Illumina Genome Analyzer IIx nifH gene nitrogen-fixing bacteria
分类号:
S154.3
DOI:
10.19675/j.cnki.1006-687x.2017.06041
摘要:
土壤微生物是土壤生态系统的重要组成部分,为了解稻田综合种养殖模式的土壤细菌尤其是功能型菌群的群落结构特征,采用高通量测序技术对稻鳖共作组(R-T)与稻田单作组(R-M)两种种养模式下根系土壤微生物(nifH基因)多样性特征进行比较. 结果显示,稻田根系土壤中,属分类级别的固氮菌主要为慢生根瘤菌属(Bradyrhizobium)、地杆菌属(Geobacter)、脱硫弧菌属(Desulfovibrio)、除硫单胞菌属(Desulfuromonas)、嗜盐红螺菌属(Halorhodospira)、固氮菌属(Azotobacter)、脱硫球茎菌属(Desulfobulbus)、甲基单胞菌属(Methylomonas)、固氮螺菌属(Azospirillum)、Pseudacidovorax、Anaeromyxobacter、Sideroxydans共12类. R-T组根系土壤固氮菌的多样性指数高于R-T组,为6.85 ± 0.07. 稻田根系土壤固氮菌在不同种养模式间的差异性占23.86%,在不同水稻生长时期的差异性占16.97%. 本研究表明,水稻的生长时期与不同种养模式对稻田根系土壤固氮微生物均具有影响,且生长时期对稻田根系土壤固氮微生物的影响更大. (图5 表3 参25)
Abstract:
oil microorganisms are an important part of the soil ecosystem, but studies on the structure of the soil bacterial community, especially the related genes, is lacking. This study aimed to investigate the structure of soil microorganisms and the connection between the soil microorganisms and different farming systems. We used the Illumina Genome Analyzer IIx to compare root soil bacteria (nifH gene) diversity characteristics between the rice-turtle co-culture system (R-T) and rice monoculture system (R-M), aiming to reveal the structural characteristics of soil microorganisms, and the connection between the soil microorganisms and different farming systems. The results of High Throughput Sequencing of nitrogen-fixing bacteria showed that the main nitrogen-fixing bacteria were classified as Bradyrhizobium, Geobacter, Anaeromyxobacter, Desulfovibrio, Sideroxydans, Desulfuromonas, Halorhodospira, Azotobacter, Desulfobulbus, Methylomonas, Pseudacidovorax, and Azospirillum. The diversity index of nitrogen-fixing bacteria in the root soil of R-T was higher than that of R-M, which was 6.85–0.07. The difference of soil nitrogen-fixation bacteria in the root soil of rice paddy was 23.86% among the different farming systems, and the difference in the different rice growth period was 16.97%. In summary, the rice growth period and different farming systems both have an influence on the nitrogen-fixation bacteria in paddy soil; the influence of the growth period on the nitrogen-fixation bacteria in the paddy soil was greater.

参考文献/References:

1 Xie J, Hu L, Tang J, Wu X, Lia N, Yuan Y, Yang H, Zhang J, Luo S, Chen X. Ecological mechanisms underlying the sustainability of the agricultural heritage rice-fish coculture system [J]. PNAS, 2011, 108 (50): 1381-7
2 Hu L, Zhang J, Ren W, Guo L, Cheng Y, Li J. Can the co-cultivation of rice and fish help sustain rice production? [J]. Sci Rep, 2016, 6: 28728
3 李嘉尧, 常东, 李柏年, 吴旭干, 朱泽闻, 成永旭. 不同稻田综合种养模式的成本效益分析[J]. 水产学报, 2014, 38 (9): 1431-1438 [Li JY, Chang D, Li BN, Wu XG, Zhu ZW, Chen YX. Benefit-cost analysis of different rice-based production systems [J]. J Fish China, 2014, 38 (9): 1431-1438]
4 赵兴武. 中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2016 [Zhao XW. China Fishery Statistical Yearbook [M]. Beijing: China Agriculture Press, 2016]
5 张丽梅, 方萍, 朱日清. 禾本科植物联合固氮研究及其应用现状展望[J]. 应用生态学报, 2004, 15 (9): 1650-1654 [Zhang ML, Fang P, Zhu RQ. Recent advances in research and application of associated nitrogen-fixation with graminaceous plants [J]. Chin J Appl Ecol, 2004, 15 (9): 1650-1654
6 Steenwerth KL, Jackson LE, Calderón FJ, Stromberg MR, Scow KM. Soil microbial community composition and land use history in cultivated and grassland ecosystems of coastal California [J]. Soil Biol Biochem, 2002, 34 (11): 1599-1611
7 靳振江, 邰继承, 潘根兴, 李恋卿, 宋祥云, 谢添, 刘晓雨, 王丹. 荆江地区湿地与稻田有机碳、微生物多样性及土壤酶活性的比较[J]. 中国农业科学, 2012, 45 (18): 3773-3781 [Jin J, Tai JC, Pan GX, Li LQ, Song XY, Xie T, Liu XY, Wang D. Comparison of soil organic carbon, microbial diversity and enzyme activity of wetlands and rice paddies in Jingjiang Area of Hubei, China [J]. Sci Agric Sin, 2012, 45 (18): 3773-3781]
8 Ingvild W, Torsten E, Zheng W, Ulla R. Variation in the active diazotrophic community in rice paddy-nifH PCR-DGGE analysis of rhizosphere and bulk soil [J]. Appl Soil Ecol, 2008, 39 (1): 65-75
9 王思潮, 曹凑贵, 李成芳, 熊又升, 汪金平. 耕作模式对冷浸田水稻产量和土壤特性的影响[J]. 中国生态农业学报, 2014, 22 (10): 1165-1173 [Wang SC, Cao CG, Li CF, Xiong YS, Wang JP. Effects of cultivation patterns on rice yield and soil properties in cold waterlogged paddy fields [J]. Chin J Eco-Agric, 2014, 22 (10): 1165-1173]
10 王秀呈. 稻—稻—绿肥长期轮作对水稻土壤及根系细菌群落的影响[D]. 北京: 中国农业科学院, 2015 [Wang XC. The influence of long term rice-rice-green manure rotation on rice bulk soil and root-associated bacteria [D]. Beijing: Chinese Academy of Agricultural Sciences, 2015]
11 Deslippe JR, Egger KN. Molecular diversity of nifH genes from bacteria associated with high arctic dwarf shrubs [J]. Microbial Ecol, 2006, 51 (4): 516-525
12 Wang Y, Sheng HF, He Y, Wu JY, Jiang YX, Tam FY, Zhou HW. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of Illumina Tags [J]. AEM, 2012, 78 (23): 8264
13 Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy [J]. AEM, 2007, 73(16): 5261-5267
14 Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Gl?ckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools [J]. Nucleic Acids Res, 2013, 41 (D1): D590-D596
15 Albanese D, De FC, Cavalieri D, Donati C. Explaining diversity in metagenomic datasets by phylogenetic-based feature weighting [J]. PLoS Comput Biol, 2015, 11 (3): e1004186
16 Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett W S, Huttenhower C. Metagenomic biomarker discovery and explanation [J]. Genome Biol Evol, 2011, 12 (6): R60
17 韩斌, 孔继君, 邹晓明, 巩合德.生物固氮研究现状和展望[J]. 山西农业科学, 2009, 37 (10): 86-89, 85 [Han B, Kong JJ, Zou XM, Gong HD. The evolvement and expectation of biological nitrogen fixation [J]. J Shanxi Agric Sci, 2009, 37 (10): 86-89, 85]
18 袁梅, 谭适娟, 孙建光. 水稻内生固氮菌分离鉴定、生物特性及其对稻苗镉吸收的影响[J]. 中国农业科学, 2016, 49 (19): 3754-3768 [Yuan M, An SJ, Sun JG. Isolation and biological properties of endophytic diazotrophs from rice and their influences on rice seedling Cd accumulation [J]. Sci Agric Sin, 2016, 49 (19): 3754-3768]
19 林利红, 王延平, 王帅. 稻田养蟹对水稻土壤理化性质的影响研究[J]. 辽宁农业科学, 2016 (3): 43-47 [Lin LH, Ang YP, Wang S. Effects of rice-crab model on physical and chemical properties of rice soil [J]. J Liaoning Agric Sci, 2016 (3): 43-47]
20 李娜娜. 中国主要稻田种养模式生态分析[D]. 杭州: 浙江大学, 2013 [Li NN. Ecological analysis of representative rice-based ecosystems in China [D]. Hangzhou: Zhejiang University, 2013]
21 张晶, 张惠文, 苏振成, 李新宇, 张成刚. 长期有机污水灌溉对土壤固氮细菌种群的影响[J]. 农业环境科学学报, 2007, 26 (2): 662-666 [Zhang J, Zhang HW, Su ZC, Li XY, Zhang CG. The Effect of long-term organic wastewater irrigation on the soil nitrogen-fixing bacteria population [J]. J Agro-Environ Sci, 2007, 26 (2): 662-666]
22 R?sch C, Mergel A, Bothe H. Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil [J]. Appl Environ Microb, 2002, 68 (8): 3818-3829
23 陈强, 陈文新, 张小平, 李登煜, Lindstrom K. 省葛藤属根瘤菌的遗传多样性研究[J]. 中国农业科学, 2004, 37 (11): 1641-1646 [Chen Q, Chen WX, Zhang XP, Li DY. Genetic diversity of rhizobia isolated from Pueraria spp. in Sichuan, China [J]. Sci Agric Sin, 2004, 37 (11): 1641-1646]
24 杨素萍, 赵春贵, 曲音波, 钱新民. 一株极端环境光合细菌的生理特性研究[J]. 水生生物学报, 2002, 26 (3): 221-225 [The isolation and characterization of Halorhodospira sp., a halophilc and alkaliphilc photosynthetic bacterium [J]. Acta Hydrob Sin, 2002, 26 (3): 221-225]
25 赖欣, 张永生, 赵帅, 李刚, 杨殿林. 转基因大豆对根际固氮细菌群落多样性的影响[J]. 生态学杂志, 2010, 29 (9): 1736-1742 [Lai X, Zhang YS, Zhao S, Li G, Yang DL. Effects of transgenic soybean on the diversity of nitrogen-fixing bacteria in rhizosphere soil [J]. Chin J Ecol, 2010, 29 (9): 1736-1742]

更新日期/Last Update: 2018-06-30