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[1]单明娟,秦华,陈俊辉,等.两种间作体系对丛枝菌根真菌侵染及多氯联苯去除的影响[J].应用与环境生物学报,2018,24(03):470-477.[doi:10.19675/j.cnki.1006-687x.2017.07001]
 SHAN Mingjuan,QIN Hua,et al.Effects of two different intercropping systems on arbuscular mycorrhizal fungal colonization and polychlorinated biphenyl (PCB) dissipation[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):470-477.[doi:10.19675/j.cnki.1006-687x.2017.07001]
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两种间作体系对丛枝菌根真菌侵染及多氯联苯去除的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
470-477
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
Effects of two different intercropping systems on arbuscular mycorrhizal fungal colonization and polychlorinated biphenyl (PCB) dissipation
作者:
单明娟秦华陈俊辉徐秋芳
1浙江省土壤污染生物修复重点实验室 临安 311300 2浙江农林大学环境与资源学院 临安 311300
Author(s):
SHAN Mingjuan1 2 QIN Hua1 2** CHEN Junhui1 2 & XU Qiufang1 2
1 Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, Lin’an 311300, China 2 School of Environmental and Resource Sciences, Zhejiang A&F University, Lin’an 311300, China
关键词:
多氯联苯间作丛枝菌根真菌细菌丰度细菌群落结构
Keywords:
polychlorinated biphenyls intercropping arbuscular mycorrhizal fungi 16S rDNA bacterial community
分类号:
S344.2 : X172
DOI:
10.19675/j.cnki.1006-687x.2017.07001
摘要:
多氯联苯(Polychlorinated biphenyls,PCBs)是一类典型的环境有机污染物,植物与微生物的联合修复能够显著提高PCBs的降解率. 以丛枝菌根(Arbuscular mycorrhiza,AM)真菌摩西管柄囊霉(Funneliformis mosseae)M47V为供试菌种,温室盆栽条件下设置玉米/黑麦草间作、玉米/紫花苜蓿间作以及玉米单作等3个处理(均接种AM真菌),研究间作对玉米根系AM真菌侵染及土壤中PCBs去除的影响. 种植90 d后测定玉米根系AM真菌侵染率、生物量、土壤PCBs含量及同系物组成、16S rDNA基因丰度,并采用末端限制性片段长度多态性(Terminal restriction fragment length polymorphism,T-RFLP)技术分析细菌群落结构. 结果显示,间作对玉米根系AM真菌侵染率、玉米生物量和土壤碱解氮含量均有显著促进作用,对土壤细菌丰度和群落结构产生显著影响,其中玉米/紫花苜蓿间作显著提高了土壤细菌数量(P < 0.05);间作显著提高五氯联苯及总PCBs的降解率,此外玉米/黑麦草间作还显著提高三氯联苯的降解率;土壤PCBs同系物组分与细菌T-RFs片段中128 bp、148 bp片段均具有显著相关性. 本研究表明,间作与AM真菌对提高玉米生物量具有协同作用,并通过影响细菌群落结构与丰度促进土壤中多氯联苯的转化与降解,同时改变其同系物结构组成,提高PCBs修复效率. (图5 表3 参36)
Abstract:
Polychlorinated biphenyls (PCBs) are typical organic contaminants in the environment. It is indicated that plants and soil microorganisms have a positive synergistic effect on the remediation of PCB-contaminated soil. To investigate the effect of intercropping on arbuscular mycorrhizal (AM) fungal colonization and PCB remediation, a pot-cultivation experiment with two intercropping treatments, corn (Zea mays L.) / ryegrass (Lolium perenne L.) and corn/alfalfa (Medicago sativa L.), and a corn monoculture was conducted in a greenhouse. All treatments were inoculated with Funneliformis mosseae M47V. Plant biomass, root mycorrhizal colonization rate, concentration of PCBs and their homologs in soil, 16S rDNA gene abundance, and community composition measured by Terminal Restriction Fragment Length Polymorphism (T-RFLP) were determined after harvesting the plants. Intercropping significantly increased the root mycorrhizal colonization rate and plant biomass of corn (P < 0.05), as well as the available N content of the soil. A significant difference of the bacterial community composition was found among different treatments (P < 0.05). Compared with corn monoculture, corn/alfalfa intercropping significantly increased soil bacteria abundance (P < 0.05). The dissipation rates of total PCBs, as well as that of penta-chloro biphenyls were significantly increased in the intercropping treatments, when compared to the corn monoculture treatment. Moreover, corn/ryegrass intercropping has a significantly positive effect on the dissipation of tri-chloro biphenyls. Non-metric multidimensional scaling (NMDS) analysis indicated that the PCBs homologues composition were significantly correlated with the relative abundance of 128 bp and 148 bp T-RFs. Corn intercropping with ryegrass or alfalfa has a positive effect on root mycorrhizal colonization rate and plant biomass of corn. Inoculation of AM fungi in intercropping treatments significantly improved the efficiency of PCB remediation by promoting bacterial abundance and shifting the bacterial community composition. In conclusion, intercropping combined with AM fungi have positive synergistic effects on the remediation of PCB-contaminated soils.

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更新日期/Last Update: 2018-06-30