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[1]韩鹏,边勇,陈青君,等.野生白桦茸的分离鉴定及其生物学特性[J].应用与环境生物学报,2018,24(03):576-582.[doi: 10.19675/j.cnki.1006-687x.2017.08036]
 HAN Peng,BIAN Yong,CHEN Qingjun,et al.Isolation, identification, and biological characteristics of a wild Chaga mushroom[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):576-582.[doi: 10.19675/j.cnki.1006-687x.2017.08036]
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野生白桦茸的分离鉴定及其生物学特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年03期
页码:
576-582
栏目:
研究论文
出版日期:
2018-06-30

文章信息/Info

Title:
Isolation, identification, and biological characteristics of a wild Chaga mushroom
作者:
韩鹏边勇陈青君张国庆张宇轩李兵徐鑫
1北京农学院生物科学与工程学院,农业部都市农业(北方)重点实验室 北京 102206 2北京出入境检验检疫局检验检疫技术中心 北京 101312 3北京农学院植物科学技术学院 北京 102206
Author(s):
HAN Peng1 BIAN Yong2 CHEN Qingjun3 ZHANG Guoqing1** ZHANG Yuxuan1 LI Bing1 & XU Xin1
1 College of Biological Science and Engineering, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing 102206, China 2 Beijing Inspection and Quarantine Testing Center, Beijing, 101312, China 3 College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China
关键词:
桦褐孔菌菌株鉴定培养条件发酵液抗氧化
Keywords:
Inonotus obliquus strain identification culture condition fermentation broth antioxidant activity
分类号:
Q949.320.5 : TQ929
DOI:
10.19675/j.cnki.1006-687x.2017.08036
摘要:
以采集自俄罗斯的野生白桦茸菌核为原料,经组织分离获得纯培养菌株(编号F1501),进一步进行分类鉴定、最适生长条件、液体发酵产多糖及其抗氧化活性与人工驯化研究. 依据内转录间隔区ITS鉴定,确定F1501菌株属于锈革菌科纤孔菌属(Inonotus)真菌,其与桦褐孔菌(Inonotus obliquus)的相似性为99%;结合菌核、菌丝形态学特征,确定F1501菌株为桦褐孔菌. 最适培养条件研究实验表明,F1501菌株的最适碳源、氮源、C/N、生长因子、温度、pH值分别为麦芽糖、牛肉浸膏、10/1、VB2、28 ℃、8.0;以马铃薯葡萄糖(PD)培养基、10%接种量、28 ℃、150 r/min培养10 d,发酵液中多糖含量为476.32 mg/L,总抗氧化活性为0.19 mmol/L(Trolox),对羟自由基(·OH)清除率为72.7%. 以棉籽壳为主要栽培基质进行人工驯化试验,可以形成类子实体结构. 本研究获得野生桦褐孔菌菌株,其生物学特性研究结果可为野生桦褐孔菌的人工驯化和开发利用提供理论依据. (图3 表6 参26)
Abstract:
In this study, a pure culture strain (numbered as F1501) was obtained using tissue separation and purification methods from the sclerotia of Chaga mushroom from Russia. Further researches included studies on classification, optimum growth conditions of mycelia, extracellular polysaccharides from fermentation broth and their antioxidant activity, and artificial acclimation. According to the identification of the internal transcribed spacer (ITS) region, it was confirmed that F1501 was a species of the genus Inonotus and family Hymenochaetaceae, which had 99% similarity with Inonotus obliquus. F1501 was further classified as I. obliquus based on the morphological characteristics of its mycelia and sclerotia. The optimal carbon source, nitrogen source, C/N ratio, growth factor, temperature, and pH value for the growth of the F1501 strain mycelia were maltose, beef extracts, 10/1, B2, 28 ℃, and 8.0, respectively. Liquid fermentation of F1501 was performed using PD media and a 10% inoculation amount at 28 ℃ and 150 r/min for 7 d. The content of polysaccharides in the fermentation broth was 476.32 mg/L, with a total antioxidant activity of 0.19 mmol/L (Trolox) and hydroxyl free radical-scavenging activity of 72.7%. Artificial acclimation study revealed that the fruiting body-like structure was obtained using cottonseed hulls as the main substrate. In the present study, a new strain of I. obliquus and its biological characteristics were explored, which could provide a theoretical basis for the artificial acclimation and development of wild mushrooms.

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更新日期/Last Update: 2018-06-30