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[1]葛慧华,张丹丹,吴姝羽,等.Foldon介导的寡聚化类弹性蛋白多肽的相变特性[J].应用与环境生物学报,2018,24(04):853-859.[doi:10.19675/j.cnki.1006-687x.2017.09045]
 GE Huihua,ZHANG Dandan,WU Shuyu,et al.Phase transition behavior of elastin-like polypeptides oligomerized with the Foldon domain[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):853-859.[doi:10.19675/j.cnki.1006-687x.2017.09045]
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Foldon介导的寡聚化类弹性蛋白多肽的相变特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
853-859
栏目:
研究论文
出版日期:
2018-08-20

文章信息/Info

Title:
Phase transition behavior of elastin-like polypeptides oligomerized with the Foldon domain
作者:
葛慧华张丹丹吴姝羽邱 岳张光亚
华侨大学化工学院生物工程与技术系 厦门 361021
Author(s):
GE Huihua ZHANG Dandan WU Shuyu QIU Yue & ZHANG Guangya**
Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China
关键词:
类弹性蛋白多肽相变温度拓扑结构与性能关系Hofmeister离子序蛋白寡聚化
Keywords:
elastin-like polypeptides transition temperature relationship of topological structure and properties Hofmeister series protein oligomerization
分类号:
Q615 : Q51
DOI:
10.19675/j.cnki.1006-687x.2017.09045
摘要:
为了解SpyCatcher-ELPs40(C-E)相变异常的原因并考察寡聚化结构域对其相变行为的影响,将Foldon(F)分别与C-E和ELPs40(E)融合,构建拓扑结构为非共价三臂星型寡聚化的C-E-F和E-F;探究不同盐浓度和类型对其相变特性的影响,并与其他ELPs进行比较. 结果显示:融合了Foldon的ELPs40的相变温度高于线型ELPs120而低于线型ELPs40及SpyTag/SpyCatcher介导的共价三臂星型ELPs,非共价三臂星型C-E-F的相变温度变化幅度较大,与等重复数的C-E和ELPs40相比,平均分别高出28.75 ℃和35.6 ℃,尤其在NaCl浓度为0.8 mol/L时,相差分别为41.2 ℃和47.1 ℃. 同时,Na2CO3浓度在0.7 mol/L及以上时,非共价三臂星型C-E-F才会在低温下发生相变,而其在较低浓度的Na2SO4溶液中相变现象明显,不符合Hofmeister效应. 本研究报道了非线型ELPs特殊的相变行为,并证明ELPs拓扑结构会影响其相变行为,但Foldon和SpyCatcher表面电荷是更重要的因素. (图6 表1 参32)
Abstract:
To investigate the underlying mechanism of the unique phase transition behavior of SpyCatcher-ELPs40 (C-E) and the influence of the oligomerization domain on the phase transition of C-E, we constructed a non-covalent three-armed star oligomerization of C-E-F and E-F by fusing the Foldon (F) domain with SpyCatcher-ELPs40 (C-E) and ELPs40 (E). Results showed that the phase transition temperature of ELPs40 fused with Foldon was higher than that of ELPs120, whereas it was lower than those of ELPs40 and the SpyTag/SpyCatcher-mediated covalent three-armed elastin-like polypeptides (ELPs). This proved that the topology of ELPs could affect their phase transition behaviors. The phase transition temperature of C-E-F was 28.8 ℃ and 35.6 ℃ higher than those of C-E and ELPs40, respectively, although C-E-F had a similar sequence with C-E and ELPs40. When the concentration of NaCl was 0.8 mol/L, the differences in the phase transition temperatures were 41.2 ℃ and 47.1 , respectively. We could only observe the phase transition of C-E-F in the Na2CO3 solution with high concentration (≥ 0.7 mol/L); however, the phase transition could be clearly detected in the Na2SO4 solution, even when the concentration of Na2SO4 was very low. This result was obviously inconsistent with the Hofmeister series. This is the first report of non-linear ELPs that has shown this unique phase transition behavior. The possible reason was related to the charges distributed on the solvent accessible surface and the oligomeric state of C-E-F triggered by the Foldon domain.

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相似文献/References:

[1]葛慧华,黄志宏,王文研,等.以类弹性蛋白多肽为标签纯化1,3-丙二醇氧化还原酶[J].应用与环境生物学报,2013,19(04):709.[doi:10.3724/SP.J.1145.2013.00709]
 GE Huihua,HUANG Zhihong,WANG Wenyan,et al.Low Concentration of Salt Triggers Elastin-like Polypeptides Inverse Transition to Purify 1,3-propanediol Oxidoreductase[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):709.[doi:10.3724/SP.J.1145.2013.00709]
[2]葛慧华,吴姝羽,沈婷婷,等.类弹性蛋白多肽长度对不溶活性ELPs-木聚糖酶聚集体酶学性质的影响*[J].应用与环境生物学报,2016,22(05):848.[doi:10.3724/SP.J.1145.2015.12018]
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更新日期/Last Update: 2018-08-25