|本期目录/Table of Contents|

[1]黄宁,李聪娜,汤翰臣,等.甘蔗泛素结合酶基因的克隆与表达[J].应用与环境生物学报,2018,24(04):845-852.[doi:10.19675/j.cnki.1006-687x.2017.11006]
 HUANG Ning,LI Congna,TANG Hanchen,et al.Cloning and expression analysis of a ubiquitin-conjugating enzyme gene in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):845-852.[doi:10.19675/j.cnki.1006-687x.2017.11006]
点击复制

甘蔗泛素结合酶基因的克隆与表达()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
845-852
栏目:
研究论文
出版日期:
2018-08-20

文章信息/Info

Title:
Cloning and expression analysis of a ubiquitin-conjugating enzyme gene in sugarcane
作者:
黄宁李聪娜汤翰臣郑清雷凌辉陈如凯阙友雄
1福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 福州 350002 2福建农林大学教育部作物遗传育种与综合利用重点实验室 福州 350002
Author(s):
HUANG Ning 1 LI Congna 1 TANG Hanchen 1 ZHENG Qinglei 1 LING Hui 1 CHEN Rukai 1 & QUE Youxiong 1 2**
1 Ministry of Agriculture Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Ministry of Education Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
泛素结合酶甘蔗生物信息学实时荧光定量PCR甘蔗黑穗病菌水杨酸茉莉酸甲酯脱落酸
Keywords:
ubiquitin-conjugating enzyme sugarcane bioinformatics real-time quantitative PCR Sporisorium scitamineum salicylic acid (SA) methyl jasmonate (MeJA) abscisic acid (ABA)
分类号:
Q78 : S556.1
DOI:
10.19675/j.cnki.1006-687x.2017.11006
摘要:
为克隆鉴定甘蔗泛素结合酶基因(Sugarcane ubiquitin-conjugating enzyme,ScUBc E2)并探索其在激素信号通路和甘蔗与黑穗病互作过程中的作用,选择黑穗病菌胁迫下甘蔗抑制消减杂交文库(Suppression subtractive hybridization, SSH)中注释为泛素结合酶的差异表达EST序列为探针,结合电子克隆技术和RT-PCR技术,以甘蔗cDNA为模板进行泛素结合酶基因克隆. 对克隆获得的序列进行生物信息学分析,并利用qRT-PCR技术分析该基因在甘蔗根、蔗髓、叶、芽中的组织特异性表达以及在黑穗病菌、茉莉酸甲酯(Methyl jasmonate,MeJA)、脱落酸(Abscisic acid,ABA)和水杨酸(Salicylic acid,SA)胁迫下的表达情况. 最终克隆得到一条长度为699 bp的甘蔗泛素结合酶基因(ScUBc E2;GenBank accession number:KJ577594.1),该基因包含长度为447 bp的完整开放读码框,编码148个氨基酸. 生物信息学分析结果显示,ScUBc E2编码的蛋白分子量(Mr)为16.507 × 103;无信号肽,为碱性不稳定的亲水蛋白;包含4个α螺旋、4个β折叠和一些无规则卷曲;第15位氨基酸为泛素化位点,74-89位氨基酸为活性位点;在进化过程中与高粱泛素结合酶基因的亲缘关系最近. qRT-PCR分析结果表明,ScUBc E2基因组成型表达,但在芽中的表达量最高;其表达在甘蔗感黑穗病基因型ROC22中受到黑穗病菌胁迫的抑制,在黑穗病抗病基因型YC05-179中则先被抑制,后被诱导;ScUBc E2基因受MeJA及SA诱导表达,对ABA胁迫的响应不明显. 本研究表明,甘蔗ScUBc E2基因在抗病基因型和感病基因型甘蔗中存在不同表达模式,可能参与甘蔗与黑穗病菌的互作过程,有望为抗病育种分子标记提供潜在基因资源;同时,该基因受MeJA和SA外源激素胁迫后的表达模式,可为泛素-蛋白酶体途径及激素调控的信号转导在甘蔗与黑穗病菌互作过程中的作用提供一定的理论依据. (图7 表2 参48)
Abstract:
The objective of this study was to examine the biological characteristics of the sugarcane ubiquitin-conjugating enzyme (ScUBc E2) gene, its role in the hormone signaling pathway, and the interaction between sugarcane and Sporisorium scitamineum. An EST was annotated as the ubiquitin-conjugating enzyme from a sugarcane suppression subtractive hybridization (SSH) library from the infection of S. scitamineum. This EST was subsequently used as a probe for in silico cloning, and the cDNA sequence of the sugarcane ubiquitin-conjugating enzyme gene was cloned using RT-PCR and was then sequenced. The bioinformatic characteristics of this gene was analyzed, and qRT-PCR was used to detect expression in sugarcane tissues, including the root, stem pith, leaf, and bud, and when treated with S. scitamineum, methyl jasmonate (MeJA), abscisic acid (ABA), and salicylic acid (SA). Finally, a ubiquitin-conjugating enzyme gene (ScUBc E2, GenBank accession number: KJ577594.1) that was 699 bp was obtained. The sequence of ScUBc E2 contained a complete open reading frame of 447 bp that encodes 148 amino acids. The bioinformatics analysis showed that ScUBc E2 was 16.507 × 103 and an alkaline unstable hydrophilic protein with no signal peptide. It contained 4 alpha helix, 4 beta folding, and some random coils, its fifteenth amino acid was a ubiquitination site, and the 74th-89th amino acid was an active site. The phylogenetic analysis showed that ScUBc E2 had the closest genetic relationship to a homologous gene in sorghum. The qRT-PCR analysis indicated that the ScUBc E2 gene was constitutively expressed in sugarcane with the highest level in the buds. The expression of the ScUBc E2 gene was inhibited in the smut-susceptible genotype ROC22 and in the early smut-infected stage of the smut-resistant genotype YC05-179, but it was induced at the later smut-infected stage in the smut-resistant genotype YC05-179 when the sugarcane plant was infected by S. scitamineum. Moreover, the expression of the ScUBc E2 gene was induced by the treatment of MeJA and SA in the sugarcane tissue culture plantlets, but it was insensitive to ABA. This study showed that the ScUBc E2 gene was involved in the interaction of sugarcane and S. scitamineum. The different expression patterns of the ScUBc E2 gene and the smut-resistant and smut-susceptible genotypes suggest that the ScUBc E2 gene may serve as a molecular marker for disease resistance during breeding. The expression patterns of the ScUBc E2 gene under the stress of MeJA and SA could also provide a basis for understanding the role of the ubiquitin-proteasome pathway and hormone regulated signal transduction in the interaction of sugarcane and S. scitamineum.

参考文献/References:

1 Ciechanover A. The ubiquitin-proteasome pathway: on protein death and cell life [J]. EMBO J, 1998, 17 (24): 7151-7160
2 Ying M, Zhan Z, Wang W, Chen D. Origin and evolution of ubiquitin-conjugating enzymes from Guillardia theta nucleomorph to hominoid [J]. Gene, 2009, 447 (2): 72-85
3 Yao T, Ndoja A. Regulation of gene expression by the ubiquitin-proteasome system [J]. Semin Cell Dev Biol, 2012, 23 (5): 523-529
4 Gray WM, Estelle I. Function of the ubiquitin-proteasome pathway in auxin response [J]. Trends Biochem Sci, 2000, 25 (3): 133-138
5 Friedman J, Xue D. To live or die by the sword: the regulation of apoptosis by the proteasome [J]. Dev Cell, 2004, 6 (4): 460-461
6 Vierstra RD. The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins [J]. Trends Plant Sci, 2003, 8 (3): 135-142
7 Bae H, Kim WT. The N-terminal tetra-peptide (IPDE) short extension of the U-box motif in rice SPL11 E3 is essential for the interaction with E2 and ubiquitin-ligase activity [J]. Biochem Biophys Res Commun, 2013, 433 (2): 266-271
8 Ye Y, Rape M. Building ubiquitin chains: E2 enzymes at work [J]. Nat Rev Mol Cell Biol, 2009, 10 (11): 755-764
9 Bahmani R, Kim D, Lee BD, Hwang S. Over-expression of tobacco UBC1 encoding a ubiquitin-conjugating enzyme increases cadmium tolerance by activating the 20S/26S proteasome and by decreasing Cd accumulation and oxidative stress in tobacco (Nicotiana tabacum) [J]. Plant Mol Biol, 2017, 94 (4-5): 433-451
10 Cui F, Liu L, Zhao Q, Zhang Z, Li Q, Lin B, Wu Y, Tang S, Xie Q. Arabidopsis ubiquitin conjugase UBC32 is an ERAD component that functions in brassinosteroid-mediated salt stress tolerance [J]. Plant Cell, 2012, 24 (1): 233-244
11 Chung E, Cho CW, So HA, Kang JS, Chung YS, Lee JH. Overexpression of VrUBC1, a mung bean E2 ubiquitin-conjugating enzyme, enhances osmotic stress tolerance in Arabidopsis [J]. PLoS ONE, 2013, 8 (6): e66056
12 Zhou GA, Chang RZ, Qiu LJ. Overexpression of soybean ubiquitin-conjugating enzyme gene GmUBC2 confers enhanced drought and salt tolerance through modulating abiotic stress-responsive gene expression in Arabidopsis [J]. Plant Mol Biol, 2010, 72 (4-5): 357-367
13 Wan X, Mo A, Liu S, Yang L, Li L. Constitutive expression of a peanut ubiquitin-conjugating enzyme gene in Arabidopsis confers improved water-stress tolerance through regulation of stress-responsive gene expression [J]. J Biosci Bioeng, 2011, 111 (4): 478-484
14 Zhou B, Zeng L. Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach [J]. Plant Methods, 2017, 13 (1): 59
15 Cheng C, Zhang C, Yao Q, Chen K. Ubiquitin-conjugating enzyme involved in the immune response caused by pathogens invasion [J]. Int J Biol Biotech 2013, 10 (1): 1-5
16 Millyard L, Lee J, Zhang C, Yates G, Sadanandom A. The ubiquitin conjugating enzyme, TaU4 regulates wheat defence against the phytopathogen Zymoseptoria tritici [J]. Sci Rep, 2016, 6: 35683
17 Jeon EH, Pak JH, Kim MJ, Kim HJ, Shin SH, Lee JH, Kim DH, Oh JS, Oh B-J, Jung HW, Chung YS. Ectopic expression of ubiquitin-conjugating enzyme gene from wild rice, OgUBC1, confers resistance against UV-B radiation and Botrytis infection in Arabidopsis thaliana [J]. Biochem Biophys Res Commun 2012, 427 (2): 309-314
18 Unver T, Turktas M, Budak H. In planta evidence for the involvement of a ubiquitin conjugating enzyme (UBC E2 clade) in negative regulation of disease resistance [J]. Plant Mol Biol Rep, 2013, 31 (2): 323-334
19 Eini O, Dogra S, Selth LA, Dry IB, Randles JW, Rezaian MA. Interaction with a host ubiquitin-conjugating enzyme is required for the pathogenicity of a geminiviral DNA beta satellite [J]. Mol Plant Microbe Interact, 2009, 22 (6): 737-746
20 Arruda P. Genetically modified sugarcane for bioenergy generation [J]. Curr Opin Biotechnol, 2012, 23 (3): 315-322
21 Li YR, Yang LT. Sugarcane agriculture and sugar industry in China [J]. Sugar Tech, 2015, 17 (1): 1-8
22 黄宁. 黑穗病菌胁迫下甘蔗SSH文库构建及差异表达基因的克隆与分析[D]. 福建: 福建农林大学, 2014 [Huang N. Construction of suppression subtractive hybridization libraries of sugarcane challenged by Sporisorium scitamineum and cloning / analysis of several differentially expressed genes [D]. FuJian: Fujian Agriculture and Foresty University, 2014]
23 苏炜华, 黄宁, 凌辉, 刘峰, 曾瑞金, 苏亚春, 吴期滨, 高世武, 阙友雄. 甘蔗乙醇脱氢酶基因的克隆与表达[J]. 应用与环境生物学报, 2017, 23 (3): 474-481 [Su WH, Huang N, Ling H, Liu Feng, Zeng RJ, Su YC, Wu QB, Gao SW, Que YX. Cloning and expression of ScADH from sugarcane [J]. Chin J Appl Environ Biol, 2017, 23 (3): 474-481]
24 肖新换, 黄珑, 黄宁, 张玉叶, 凌辉, 刘峰, 苏炜华, 阙友雄. 甘蔗ScBAK1基因及其可变剪接体的克隆与表达分析[J]. 应用与环境生物学报, 2015, 21 (5): 872-881 [Xiao XH, Huang L, Huang N, Zhang YY, Ling H, Liu Feng, Su WH, Que YX. Cloning and expression analysis of ScBAK1 gene and its alternative spliceosome in sugarcane [J]. Chin J Appl Environ Biol, 2015, 21 (5): 872-881]
25 阙万才, 黄宁, 刘峰, 肖新换, 凌辉, 张玉叶, 苏炜华, 苏亚春, 吴期滨, 阙友雄. 甘蔗真核生物翻译起始因子5A基因的克隆与表达分析[J]. 应用与环境生物学报, 2015, 21 (6): 1120-1127 [Que WC, Huang N, Liu F, Xiao XH, Ling H, Zhang YY, Su WH, Su YC, Wu QB, Que YX. Cloning and expression analysis of a eukaryotic translation initiation factor 5A gene in sugarcane [J]. Chin J Appl Environ Biol, 2015, 21 (6): 1120-1127]
26 Que Y, Su Y, Guo J, Wu Q, Xu L. A global view of transcriptome dynamics during Sporisorium scitamineum challenge in sugarcane by RNA-seq [J]. PLoS ONE, 2015, 10 (2): e0118445
27 Ling H, Wu Q, Guo J, Xu L, Que Y. Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR [J]. PLoS ONE, 2014, 9 (5): e97469
28 阙友雄, 许莉萍, 徐景升, 张积森, 张木清, 陈如凯. 甘蔗基因表达定量PCR分析中内参基因的选择[J]. 热带作物学报, 2009, 30 (3): 274-278 [Que YX, Xu LP, Xu JS, Zhang JS, Zhang MQ, Chen RK. Selection of control genes in real-time qPCR analysis of gene expression in Sugarcane [J]. Chin J Rice Trop crop, 2009, 30 (3): 274-278]
29 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method [J]. Methods, 2001, 25 (4): 402-408
30 Spoel SH, Dong X. How do plants achieve immunity? Defence without specialized immune cells [J]. Nat Rev Immunol, 2012, 12 (2): 89-100
31 Zipfel C, Robatzek S. Pathogen-associated molecular pattern-triggered immunity: veni, vidi ... ? [J]. Plant Physiol, 2010, 154 (2): 551-554
32 Zhang Y, Zhao Z, Xue Y. Roles of proteolysis in plant self-incompatibility [J]. Annu Rev Plant Biol, 2009, 60: 21-42
33 Kurepa J, Wang S, Li Y, Smalle J. Proteasome regulation, plant growth and stress tolerance [J]. Plant Signal Behav, 2009, 4 (10): 924-927
34 Trujillo M, Shirasu K. Ubiquitination in plant immunity [J]. Curr Opin Plant Biol, 2010, 13 (4): 402-408
35 刘炎霖, 陈钰辉, 刘富中, 张映, 连勇. 水茄泛素结合酶E2基因StUBCc的克隆及黄萎病菌诱导表达分析[J]. 园艺学报, 2015, 42 (6): 1185-1194 [Liu YL, Chen YH, Liu FZ, Zhang Y, Lian Y. Cloning and expression analysis induced by verticillium wilt fungus of ubiquitin-conjugating enzyme gene StUBCc from Solanum torvum [J]. Acta Horticult Sin, 2015, 42 (6): 1185-1194]
36 蒋春苗, 黄兴奇, 付坚, 余腾琼, 钟巧芳, 李定琴, 殷富有, 张敦宇, 王玲仙, 程在全. 疣粒野生稻泛素结合酶基因的全长cDNA序列克隆与分析[J]. 作物学报, 2012, 38 (5): 808-813 [Jiang CM, Huang XQ, Fu J, Yu TQ, Zhong QF, Li DQ, Yin FY, Zhang DY, Wang LX, Cheng ZQ. Cloning and analysis on full-length cdna sequence of ubiquitin-conjugating enzyme gene from oryza meyeriana baill [J]. Acta Agron Sin, 2012, 38 (5): 808-813]
37 刘鑫, 张恒, 阚虎飞, 周立帅, 黄昊, 宋林林, 翟焕趁, 张君, 鲁国东. 水稻泛素结合酶基因家族的生物信息学与表达分析[J]. 中国水稻科学, 2016, 30 (3): 223-231 [Liu X, Zhang H, Que HF, Zhou LS, Huang H, Song LL, Zhai HC, Zhang J, Lu GD. Bioinformatic and expression analysis of rice ubiquitin-conjugating enzyme gene family [J]. Chin J Rice Sci, 2016, 30 (3): 223-231]
38 Lipka U, Fuchs R, Lipka V. Arabidopsis non-host resistance to powdery mildews [J]. Curr Opin Plant Biol, 2008, 11 (4): 404-411
39 Parish RW, Li SF. Death of a tapetum: a programme of developmental altruism [J]. Plant Sci, 2010, 178 (2): 73-89
40 Kim M, Ahn JW, Jin UH, Choi D, Paek KH, Pai HS. Activation of the programmed cell death pathway by inhibition of proteasome function in plants [J]. J Biol Chem, 2003, 278 (21): 19406-19415
41 Su Y, Xu L, Wang Z, Peng Q, Yang Y, Chen Y, Que Y. Comparative proteomics reveals that central metabolism changes are associated with resistance against Sporisorium scitamineum in sugarcane [J]. BMC Genomics, 2016, 17 (1): 800
42 Schaker PDC, Palhares AC, Taniguti LM, Peters LP, Creste S, Aitken KS, Van Sluys M-A, Kitajima JP, Vieira MLC, Monteiro-Vitorello CB. RNAseq transcriptional profiling following whip development in sugarcane smut disease [J]. PLoS ONE, 2016, 11 (9): e0162237-e0162237
43 Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense [J]. Curr Opin Plant Biol, 2002, 5 (4): 325-331
44 Asselbergh B, De Vleesschauwer D, Hofte M. Global switches and fine-tuning - ABA modulates plant pathogen defense [J]. Mol Plant Microbe Interact, 2008, 21 (6): 709-719
45 覃碧. 巴西橡胶树HbUBC22基因克隆与表达分析[J]. 中国农学通报, 2013, 29 (19): 1-8 [Qin B. Cloning and Expression of HbUBC22 in Hevea brasiliensis Muell. Arg. [J]. Chin Agric Sci Bull, 2013, 29 (19): 1-8]
46 E Z, Zhang Y, Li T, Wang L, Zhao H. Characterization of the ubiquitin-conjugating enzyme gene family in rice and evaluation of expression profiles under abiotic stresses and hormone treatments [J]. Plos One, 2015, 10 (4): e0122621
47 王园, 王甲水, 谢学立, 雷晓明, 金志强. 香蕉泛素结合酶基因与果实成熟关系的研究[J]. 园艺学报, 2010, 37 (5): 705-712 [Wang Y, Wang JS, Xie XL, Lei XM, Jin ZQ. Studies of the relationship between MaUCE1 and banana fruit ripening [J]. Acta Horticult Sin, 2010, 37 (5): 705-712]
48 蔡佳文, 金晓霞, 于丽杰, 崔柏杨, 魏迅, 董延龙. 龙葵E2泛素结合酶基因SorUBC克隆及表达特性分析[J]. 东北农业大学学报, 2016, 47 (11): 26-36 [Cai JW, Yu XX, Yu LJ, Cui BY, Wei X, Dong YL. Clone and expression pattern analysis of E2 ubiquitin-binding enzyme gene SorUBC in Solanum nigrum L [J]. J North Agric Univ, 2016, 47 (11): 26-36]

相似文献/References:

[1]罗俊,袁照年,张华,等.宿根甘蔗产量性状的稳定性分析[J].应用与环境生物学报,2009,15(04):488.[doi:10.3724/SP.J.1145.2009.00488]
 LUO Jun,YUAN Zhaonian,ZHANG Hua,et al.Stability Analysis on Yield Characters of Sugarcane Ratoon[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):488.[doi:10.3724/SP.J.1145.2009.00488]
[2]叶冰莹,邱思,周平,等.甘蔗蔗糖磷酸合成酶SPSⅡ cDNA片段克隆与表达分析[J].应用与环境生物学报,2011,17(05):673.[doi:10.3724/SP.J.1145.2011.00673]
 YE Bingying,QIU Si,ZHOU Ping,et al.Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(04):673.[doi:10.3724/SP.J.1145.2011.00673]
[3]黄祖新,黄镇,叶冰莹,等.宿根甘蔗根际土壤细菌多样性分析中培养法与非培养法比较研究[J].应用与环境生物学报,2011,17(05):742.[doi:10.3724/SP.J.1145.2011.00742]
 HUANG Zuxin,HUANG Zhen,YE Bingying,et al.Comparison of Culture-dependent and -independent Approaches for Diversity Analysis of Soil Bacteria in the Rhizosphere of Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(04):742.[doi:10.3724/SP.J.1145.2011.00742]
[4]罗俊,邓祖湖,阙友雄,等.国家甘蔗第七轮区域试验品种的丰产性及稳定性[J].应用与环境生物学报,2012,18(05):734.[doi:10.3724/SP.J.1145.2012.00734]
 LUO Jun,DENG Zuhu,QUE Youxiong,et al.Productivity and Stability of Sugarcane Varieties in the 7th Round National Regional Trial of China[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):734.[doi:10.3724/SP.J.1145.2012.00734]
[5]苏亚春,凌辉,王恒波,等.甘蔗SCoT-PCR反应体系优化与多态性引物筛选及应用[J].应用与环境生物学报,2012,18(05):810.[doi:10.3724/SP.J.1145.2012.00810]
 SU Yachun,LIN Hui,WANG Hengbo,et al.Optimization of SCoT-PCR Reaction System, and Screening and Utilization of Polymorphic Primers in Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):810.[doi:10.3724/SP.J.1145.2012.00810]
[6]肖新换,黄宁,张玉叶,等.甘蔗光合系统Ⅰ亚基O基因的克隆与表达分析[J].应用与环境生物学报,2015,21(02):208.[doi:10.3724/SP.J.1145.2014.09033]
 XIAO Xinhuan,HUANG Ning,ZHANG Yuye,et al.Cloning and expression of photosystem I subunit O gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):208.[doi:10.3724/SP.J.1145.2014.09033]
[7]肖新换,黄珑,黄宁,等.甘蔗ScBAK1基因及其可变剪接体的克隆与表达分析[J].应用与环境生物学报,2015,21(05):872.[doi:10.3724/SP.J.1145.2015.03005]
 XIAO Xinhuan,HUANG Long,HUANG Ning,et al.Cloning and expression analysis of ScBAK1 gene and its alternative spliceosome in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):872.[doi:10.3724/SP.J.1145.2015.03005]
[8]阙万才,黄宁,刘峰,等.甘蔗真核生物翻译起始因子5A基因的克隆与表达分析[J].应用与环境生物学报,2015,21(06):1120.[doi:10.3724/SP.J.1145.2015.04008]
 QUE Wancai,HUANG Ning,LIU Feng,et al.Isolation and expression of a eukaryotic translation initiation factor 5A gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):1120.[doi:10.3724/SP.J.1145.2015.04008]
[9]苏炜华# 黄 珑# 黄 宁 刘 峰 苏亚春 肖新换 凌 辉 阙友雄.甘蔗细胞色素P450还原酶基因的RT-PCR扩增与表达分析[J].应用与环境生物学报,2016,22(02):173.[doi:10.3724/SP.J.1145.2015.07029]
 SU Weihua#,HUANG Long#,HUANG Ning,et al.RT-PCR amplification and expression analysis of a cytochrome P450 reductasegene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):173.[doi:10.3724/SP.J.1145.2015.07029]
[10]苏炜华,黄宁,凌辉,等.甘蔗乙醇脱氢酶基因的克隆与表达分析[J].应用与环境生物学报,2017,23(03):474.[doi:2016.0704]
 SU Weihua,HUANG Ning,LING Hui,et al.Cloning and expression of ScADH from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):474.[doi:2016.0704]

更新日期/Last Update: 2018-08-25