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[1]刘梅,原居林,何海生,等.微藻在南美白对虾养殖废水中的生长及净化效果[J].应用与环境生物学报,2018,24(04):866-872.[doi:10.19675/j.cnki.1006-687x.2017.11012]
 LIU Mei,YUAN Julin,HE Haisheng,et al.Removal of nitrogen and phosphorus by eight strains of microalgae and their growth characteristics in Penaeus vannamei sewage[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):866-872.[doi:10.19675/j.cnki.1006-687x.2017.11012]
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微藻在南美白对虾养殖废水中的生长及净化效果()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年04期
页码:
866-872
栏目:
研究论文
出版日期:
2018-08-20

文章信息/Info

Title:
Removal of nitrogen and phosphorus by eight strains of microalgae and their growth characteristics in Penaeus vannamei sewage
作者:
刘梅原居林何海生倪蒙顾志敏
1农业部淡水渔业健康养殖重点实验室,浙江省淡水水产遗传育种重点实验室,浙江省淡水水产研究所 湖州 313001 2上海海洋大学水产与生命科学学院 上海 201306
Author(s):
LIU Mei1 YUAN Julin1 HE Haisheng2 NI Meng1 & GU Zhimin12*
1 Ministry of Agriculture Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic?and Breeding??of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China 2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
关键词:
微藻南美白对虾养殖尾水水质净化
Keywords:
microalgae Penaeus?vannamei aquaculture?wastewater water?quality?purification nitrogen phosphorus
分类号:
X173 : X714
DOI:
10.19675/j.cnki.1006-687x.2017.11012
摘要:
为了解不同微藻对南美白对虾养殖尾水的净化作用,通过室内微藻培养试验,分析8株微藻在南美白对虾养殖尾水中的生长性能及不同时间内其对氮磷的去除效果. 结果表明,8株藻均能在南美白对虾养殖尾水中生长,其中铜绿微囊藻、衣藻和蛋白核小球藻生长较好,平均相对生长速率分别为0.309 3、0.246 9和0.215 5. 8种藻对总氮(TN)、总磷(TP)的去除效果差异较大,其中铜绿微囊藻、衣藻和蛋白核小球藻对TN去除效果较好,培养结束时去除率分别达到74%、69%和60%;铜绿微囊藻和衣藻均具有较好的TP去除效果,去除率达60%以上,其次为蛋白核小球藻. 不同藻类对不同形态氮的吸收存在较大差异,铜绿微囊藻和衣藻具有较好的硝态氮去除效果,去除率达到70%左右;衣藻对氨氮具有较快且持久的去除率,去除率高达100%,铜绿微囊藻和蛋白核小球藻稍慢,而斜生栅藻、针杆藻和舟形藻最慢,在培养16 d后,去除率均达到90%以上;隐藻和蛋白核小球藻对亚硝态氮具有较好的去除效果,培养至第8天时即达到80%的去除率,且隐藻去除效果较持久. 本研究获得了不同藻类对对虾养殖尾水的吸收利用特点,可为南美白对虾养殖过程中藻相的定向培育及养殖尾水净化提供科学参考依据. (图4 表2 参42)
Abstract:
Microalgae are the most important primary productive forces in shrimp aquaculture systems. Microalgae not only provide oxygen and natural food for aquaculture objects, but they also absorb nitrogen (N) and phosphorus (P) to reduce water eutrophication. However, there are great differences in N and P absorption among different strains of microalgae. To maintain the sustainable development of shrimp aquaculture, the growth performances of eight microalgal strains in Penaeus vannamei sewage and N and P removal rates were investigated under laboratory conditions. The results indicated that the eight microalgal strains could reduce the N and P content in P. vannamei sewage to some extent. Microcystis aeruginosa, Chlamydomonas sp., and Chlorella pyrenoidosa grew very well, with average growth rates of 0.309 3, 0.246 9, and 0.215 5, respectively. There were significant differences in the removal efficiency among the different strains. The removal rates of total N by M. aeruginosa, Chlamydomonas sp., and C. pyrenoidosa were 74%, 69%, and 60%, respectively, at the end of the experiment, which were higher than the other species. M. aeruginosa and Chlamydomonas sp. had better total P removal efficiency than those of the other microalgal strains and removal rates were greater than 60%, and the second highest total P removal efficiency was by C. pyrenoidosa. Different types of microalgal strains had different absorption rates of different morphological nitrogen. M. aeruginosa and Chlamydomonas sp. had the highest nitrate nitrogen removal rate (approximately 70%). Chlamydomonas sp. had a fast and persistent removal rate of ammonia nitrogen, with the removal rate being as high as 100%. The removal efficiency of M. aeruginosa and C. pyrenoidosa were a little slower, and those of Scenedesmus obliquus, Synedra sp., and Navicula graciloides were the slowest. After 16 d, the removal rate reached more than 90%. Cryptomonas obovate and C. pyrenoidosa displayed the best removal rate of nitrite nitrogen, and the removal rate reached 80% on day 8, and the removal rate of C. obovata was more persistent. These results can provide scientific reference for the orientation and use of microalgae to remove pollutants in tailings water from shrimp aquaculture systems.

参考文献/References:

1 Hugues L, Sébastien H, Kento N, Claude C, Martine R. Response of phytoplankton to organic enrichment and shrimp activity in tropical aquaculture ponds: a mesocosm study [J]. Aquat Microb Ecol, 2017, 80: 105-122
2 Gao J, Zuo H, Yang L, He J, Niu S, Weng S, He J, Xu X. Long-term influence of cyanobacterial bloom on the immune system of Litopenaeus vannamei. Fish Shellfish Immun, 2017,61: 79-854
3 Mariscal MM, Páez-Osuna F. Mass balances of nitrogen and phosphorus in an integrated culture of shrimp (Litopenaeus vannamei) and tomato (Lycopersicon esculentum Mill) with low salinity groundwater: a short communication [J]. Aquacul Eng, 2014, 58: 107–112
4 彭聪聪, 李卓佳, 曹煜成, 刘孝竹,胡晓娟. 凡纳滨对虾半集约化养殖池塘微藻优势种变动规律及其对养殖环境的影响[J]. 海洋环境科学, 2011, 30 (2): 193-198 [Peng CC, Li ZJ, Cao YC, Liu XZ, Hu XJ. Change of dominant species of planktonic microalgae in Litopenaeus vannamei’s semi - intensive culture ponds and its impact on the culture environment [J]. Mar Environ Sci, 2011, 30 (2): 193-198]
5 Van RJ. Waste treatment in recirculating aquaculture systems [J]. Aquacul Eng, 2013, 53: 49-56
6 韩士群, 严少华, 范成新. 水产养殖废水循环利用及多余藻类生物量资源化[J]. 自然资源学报, 2008, 23 (4): 560-568 [Han SQ, Yan SH, Fan CX. Recycling of aquacultural wastewater and reusing the resource of redundant alage biomass [J]. J Nat Resour, 2008, 23 (4): 560-568]
7 吴定心. 微生物制剂对南美白对虾养殖体系微生态的影响及其与藻类关系的研究[D]. 武汉: 华中农业大学, 2016 [Wu DX. Studies of effects on Litopenaeus vannamei bacterial ecology caused by probiotics application and mechanism of interaction between microalgae and probiotics [D]. Wuhan: Huazhong Agricultural University, 2016]
8 杨坤, 李静, 赵秀侠, 朱斌, 卢文轩. 栅藻和小球藻在4种养殖废水中的生长及净化效果对比研究[J]. 环境工程学报, 2017, 11 (7): 4411-4418 [Yang K, Li J, Zhao X, Zhu B, Lu W. A comparative study on growth and purification efficiency of Scenedesmus obliquus and Chlorella vulgaris in four kinds of breeding wastewater [J]. Chin J Environ Eng, 2017, 11(7): 4411-4418]
9 Oswald WJ, Gotaas HB, Golueke CG, Kellen WR. Algae in waste treatment [J]. Sew Ind Wastes, 1957, 29 (4): 437-457
10 Porrello S, Lenzi1 M, Tomassetti P, Persia E, Finoia MG, Mercalati I. Reduction of aquaculture wastewater eutrophication by phytotreatment ponds system II. Nitrogen and phosphorus content in macroalgae and sediment [J]. Aquaculture, 2003, 219(1-4): 531-544
11 Arnaud MF. The role of microalgae in aquaculture: situation and trends [J]. J Appl Phycol, 2000, 12 (3-5): 527-534
a) 闫志强, 宋本如, 刘黾, 刘燕, 吴小业, 李洁珊, 林燕珍, 冯翊果, 陈章和, 赵建刚. 5种沉水植物对斜生栅藻的化感作用[J]. 应用与环境生物学报, 2015, 21 (1): 75-79 [Yan ZQ, Song BR, Liu Y, Liu Y, Wu XY, Li JS, Lin YZ, Feng XG, Chen ZH, Zhao JG. Allelopathic effect of five submerged macrophytes on Senedesmus obliquus [J]. Chin J Appl Environ Biol, 2015, 21 (1): 75-79]
12 沈明明, 李健, 王清印, 葛红星, 刘萍, 常志强. 凡纳滨对虾(Litopenaeus vannamei)工厂化养殖系统微藻的群落特征分析[J]. 渔业科学进展, 2017, 38 (5): 64-72 [Shen M, LI J, Wang Q, GE H, Liu P, Chang Z. Analysis of the microalgae community in industrial aquaculture ponds of pacific white shrimp (Litopenaeus vannamei) [J]. Progr Fish Sci, 2017, 38 (5): 64-72]
13 张继平, 郭照良. 小球藻对降低南美白对虾养殖水体中亚硝酸盐氮含量的研究[J]. 水产科学, 2006, 25 (10): 517-519 [Zhang JP, Guo ZL. Reduction of the concentration of nitrite in cultivation water of Litopenaeus vannamei by Chlorella [J]. Fish Sci, 2006, 25 (10): 517-519]
14 郑莲, 黄翔鹄, 刘楚吾, 陈艳娟. 微绿球藻固定化培养及其对对虾养殖水质调控[J]. 海洋科学, 2005, 29 (6): 4-8 [Zheng L, Huang XH, Liu CW, Chen YJ. Immobilization of Nannnochloris oculata in water quality control in shrimp mariculture [J]. Sea Sci, 2005, 29 (6): 4-8]
15 黄翔鹄, 李长玲, 郑莲, 刘楚吾, 王瑞旋. 固定化微藻对虾池弧菌数量动态的影响[J]. 水生生物学报, 2005, 29 (6): 684-688 [Huang XH, Li CL, Zhen L, Wang RX. Effects of the immobilized microalgae on the quantity dynamics of VIBRIO in the shrimp ponds [J]. Acta Hydrobiol Sin, 2005, 29 (6): 684-688]
16 邱昌恩, 况琪军, 刘国祥, 胡征宇. 不同氮浓度对绿球藻生长及生理特性的影响[J]. 中国环境科学, 2005, 25 (4): 408-411 [Yue CN, Kuang QJ, Liu GX, Hu ZY. Influence of different nitrogen concentrations on the growth and physiological characteristics of Chlorococcum sp. [J]. China Environ Sci, 2005, 25 (4): 408-411]
17 丁彦聪, 高群, 刘家尧, 衣艳君,刘建国,林伟. 环境因子对蛋白核小球藻生长的影响及高产油培养条件的优化[J]. 生态学报, 2011, 18 (31): 5307-5315 [Ding Y C, Gao Q, Liu J Y, Yi Y J, Liu J G, Lin W. Effect of environmental factors on growth of Chlorella sp. and optimization of culture conditions for high oil production [J]. Acta Ecol Sin, 2011, 31 (18) : 5307-5315]
18 田立丹, 王素英. 常见废水与螺旋藻生长的适配性研究[J]. 水产科学, 2011, 30 (6): 323-330 [Tian LD, Wang, SY. The adaptation of common wastewater to alga Spirulina [J]. Fish Sci, 2011, 30 (6): 323-330]
19 欧阳峥嵘, 温小斌, 耿亚红, 梅洪 胡鸿钧 张桂艳 李夜光. 光照强度、温度、pH、盐度对蛋白核小球藻(Chlorella)光合作用的影响[J]. 武汉植物学研究, 2010, 28 (1): 49-55 [Ouyang ZR, Wen XH, Gen YH, Mei H, Hu HG, Zhang GY, Li YG. The effect of light intensities, temperature, pH and Salinities on photosynthesis of Chlorella [J]. J Wuhan Bot Res, 2010, 28 (1): 49-55]
20 刘霞, 陆晓华, 陈宇炜. 太湖浮游硅藻时空演化与环境因子的关系[J]. 环境科学学报, 2012, 32 (4): 821-827 [Liu X, Lu XH, Chen YW. Long-term evolution of planktonic diatoms and their relationships with environmental factors in Lake Taihu [J]. Acta Sci Circum, 2012, 32 (4): 821-827]
21 吴攀, 邓建明, 秦伯强, 马建荣,张运林. 水温和营养盐增加对太湖冬、春季节藻类生长的影响[J]. 环境科学研究, 2013, 26 (10) : 1064-1071 [Wu P, Deng JM, Qin BQ, Ma JR, Zhang YL. Effects of enhanced water temperature and nutrient concentration on algal growth in winter and spring season inLake Taihu, China [J]. Res Environ Sci, 2013, 26 (10): 1064-1071]
22 Schindler DW. Evolution of phosphorus limitation in lakes. Natural mechanisms compensate for deficiencies of nitrogen and carbon in eutrophied lakes) [J]. Science, 1977, 195: 260-262
23 Jackson DF. Ecological Factors Governing Blue-green Algal Blooms: Series 117 [M]. Indiana: Purdue University Extension, 1964: 402-420
24 Robarts RD, Zohary T. Temperature effects on photosynthetic capacity, respiration and growth rates of bloom-forming can bacterial [J]. N Z J Mar Fresh Water Res, 1987, 21: 391-399
25 Pavlo B, Debora CK,Natalie B, Laila KN, Celine C, Steven C, Su C,Tang Y, Michael JB, Bouwer EJ. Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater [J]. Algal Res, 2017, 19: 278-290
26 许海, 朱广伟, 秦伯强, 高光. 氮磷比对水华蓝藻优势形成的影响[J]. 中国环境科学, 2011, 31 (10): 1676-1683 [Xu H, Zhu GW, Qin BQ, Gao G. Influence of nitrogen-phosphorus ratio on dominance of bloom-forming cyanobacteria (Microcystis aeruginosa) [J]. China Environ Sci, 2011, 31 (10) : 1676-1683]
27 Smith VH. Low nitrogen to phosphorus ratios favor dominance by blue-green algae in lake phytoplankton [J]. Science, 1983, 225: 669-671
28 马健荣, 邓建明, 秦伯强, 龙胜兴. 湖泊蓝藻水华发生机理研究进展[J]. 生态学报, 2013, 33 (10): 3020-3030 [Ma JR, Deng JM, Qin BQ, Long SX. Progress and prospects on cyanobacteria bloom-forming mechanism in lakes [J]. Acta Ecol Sin, 2013, 33 (10): 3020-3030]
29 秦伯强, 王小冬, 汤祥明, 冯胜, 张运林. 太湖富营养化与蓝藻水华引起的饮用水危机-原因与对策[J]. 地球科学进展, 2007, 22 (9): 896-906 [Qin BQ, Wang XD, Tang XM, Feng S, Zhang YL. Drinking water crisis caused by eutriphication and cyanobacterial bloom in Lake Taihu cause and measurement [J]. Adv Eearth Sci, 2007, 22 (9): 896-906]
30 孔繁翔, 宋立荣. 蓝藻水华过程及其环境特征研究[M]. 北京: 科学出版社, 2011 [Kong FX, Song LR. Cyanobacterial Bloom Process and Its Environmental Characteristics [M]. Beijing: Science Press, 2011]
31 Yang DF, Chen ST, Jun HF, Wu JP, Huang H. Magnitude order of light, temperature and nutrients on phytoplankton growth affect [J]. Mar Environ Sci, 2007, 3: 201-207
32 Sylvia B, Frances RP. Freshwater bloom-forming cyanobacteria and anthropogenic change [J]. Limnol Oceanoogr, 2017, 7 (2): 1-62
33 万蕾, 朱伟, 赵联芳. 氮磷对铜绿微囊藻和栅藻生长及竞争的影响[J]. 环境科学, 2007, 28 (6): 1230-1235 [Wan L, Zhu W, Zhao LF. Effect of nitrogen and phosphorus on growth and competition of M. aeruginosaand and S. quadricauda [J]. Environ Sci, 2007, 28 (6): 1230-1235]
34 Tam NFY, Wong YS. Wastewater nutrient removal by Chlorella pyrenoidosa and Scenedesmus sp [J]. Envion Pollut, 1989, 58: 19-26
35 钱善勤 , 孔繁翔 , 张民 , 于洋, 史小丽. 铜绿微囊藻和蛋白核小球藻对不同形态有机磷的利用及其生长[J]. 湖泊科学, 2010, 22 (3): 411-415 [Qian SQ, Kong FX, Zhang M, Yu Y, Shi XL. Utilization of dissolved organic phosphorus and the growth of Microcystis aeruginosa and Chlorella pyrenoidosa [J]. J Lake Sci , 2010, 22 (3): 411-415]
36 包苑榆, 钟萍, 韦桂峰, 郭海森 , 刘正文. 基于15N稳定同位素技术的斜生栅藻对硝氮和氨氮吸收研究[J]. 水生态学杂志, 2011, 32 (3): 16-20 [Bao WY, Zhong P, Wei GF, Guo HS, Liu ZW. Study on uptake of nitrate and ammonium by Scendesmus obliquus based on 15N stable isotope technique [J]. J Hydroecol, 2011, 32 (3): 16-20]
37 Ohmori M, Ohmori K, Strotmann H. Inhibition of nitrate uptake by ammonia in a blue-green alga, Anabaena cylindrical [J]. Arch Microbiol, 1977, 114: 225-229
38 Guerrero MG, Vega JM, Losads M. The assimilatory nitrate reducing system and its regulation [J]. Annu Rev Plant Phsol Plant Mole Biol, 1981, 32: 169-204
39 陈春云, 庄源益, 方圣琼. 小球藻对养殖废水中N、P的去除研究[J]. 海洋环境科学, 2009, 28 (1): 9-11 [Study on N, P removed in culturing wastewater by Chlorella [J]. Mar Environ Sci, 2009, 28 (1): 9-11]
40 栗越妍, 孟睿, 何连生, 席北斗. 净化水产养殖废水的藻种筛选[J]. 环境科学与技术, 2010, 33 (6): 67-70 [Li YY, Meng R, He LS, Xi BD. Screening of algae for purifying aquaculture wastewater [J]. Environ Sci Technol, 2010, 33 (6): 67-70]
41 刘林林, 黄旭雄, 危立坤, 曾蓓蓓,穆亮亮,刘志坚,蔡志武. 15株微藻对猪场养殖污水中氮磷的净化及其细胞营养分析[J]. 环境科学学报, 2014, 34 (8): 1986-1994 [Liu LL, Huang XX, Wei LK, Zeng BB, Mu LL, Liu ZJ, Cai ZW. Removal of nitrogen and phosphorus by 15 strains of microalgae and their nutritional values in piggery sewage [J]. Acta Sci Circum, 2014, 34 (8): 1986-1994]
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更新日期/Last Update: 2018-08-25