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[1]任海伟,孙安琪,马延琴,等.添加乙酸对干玉米秸秆与白菜废弃物混贮品质的影响[J].应用与环境生物学报,2018,24(05):1087-1096.[doi:10.19675/j.cnki.1006-687x.2017.11016]
 REN Haiwei,SUN Anqi,et al.Effects of different doses of acetic acid on mixed storage quality of dry corn stalk and Chinese cabbage waste[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1087-1096.[doi:10.19675/j.cnki.1006-687x.2017.11016]
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添加乙酸对干玉米秸秆与白菜废弃物混贮品质的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1087-1096
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Effects of different doses of acetic acid on mixed storage quality of dry corn stalk and Chinese cabbage waste
作者:
任海伟孙安琪马延琴王昱范文广李志忠孙文斌沈佳莉刘菲菲
1兰州理工大学生命科学与工程学院 兰州 7300502甘肃省生物质能与太阳能互补供能系统重点实验室 兰州 730050
Author(s):
REN Haiwei1 2 SUN Anqi1 MA Yanqin1 WANG Yu2 FAN Wenguang1 LI Zhizhong1** SUN Wenbin1 SHEN Jiali1 & LIU Feifei1
1 School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China 2 Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou 730050, China
关键词:
干玉米秸秆白菜废弃物乙酸混贮发酵品质高通量测序
Keywords:
dry maize straw cabbage waste acetic acid mixed storage fermentation quality high-throughput sequencing
分类号:
X712 : Q920
DOI:
10.19675/j.cnki.1006-687x.2017.11016
摘要:
为促进干秸秆的跨季节贮存及尾菜资源化利用,利用青贮原理将干玉米秸秆与白菜废弃物进行混合贮存发酵,探讨不同添加量乙酸对二者混贮发酵品质的影响,并通过Miseq高通量测序技术解析发酵过程中的微生物多样性. 设置ME组(无乙酸添加)为对照组,AA组(添加量0.3%)和AB组(添加量0.6%)为2个乙酸处理组,18 ± 1 ℃恒温密闭混贮60 d,间隔30 d分析其化学组分、发酵品质及微生物多样性. 结果表明,AA组贮存60 d时的干物质、可溶性碳水化合物和粗蛋白含量均显著高于ME组,酸性洗涤纤维和酸性洗涤木质素含量显著下降,半纤维素、纤维素和综纤维素含量显著增加. AB组在30 d和60 d时pH均显著低于ME组,乳酸含量在30 d时显著增加,60 d时显著降低. AA组和AB组中乳酸/乙酸、乳酸/总有机酸及氨氮/总氮值均低于ME组. 混贮发酵期间ME组、AA组和AB组的门水平细菌主要为Proteobacteria和Firmicutes,属水平细菌包括Lactobacillus、Paralactobacillus、Enterobacter、Pediococcus、Flavobacterium、Chryseobacterium、Pedobacter、Sphingomonadaceae、Erwinia等,其中Lactobacillus、Paralactobacillus和Enterobacter为优势菌. 与ME组相比,AA组和AB组中腐败菌Enterobacter丰度呈下降趋势;2个乙酸处理组的总乳酸菌丰度高于ME组,但乳酸菌多样性较ME组有所减少,主要包括Lactobacillus、Paralactobacillus和Pediococcus等乳酸菌属. 总之,干玉米秸秆与白菜废弃物能以适宜比例混贮60 d不变质,尽管添加乙酸后的乳酸发酵强度有所抑制,但低剂量乙酸更有助于保存干物质、可溶性碳水化合物、粗蛋白、综纤维素等有机组分,建议乙酸添加量为0.3%. (图3 表5 参43)
Abstract:
In order to achieve the trans-seasonal storage of dry maize straw (DMS) and utilization of vegetable wastes, the mixed airtight wet-storage of DMS and cabbage waste (CW) were implemented at 18 ± 1 ℃ for 60 days based on silage theory. The effects of different doses of acetic acid on the storage and fermentation quality of DMS and CW were investigated. The dynamics of their microbial community diversity during storage were also investigated using Illumina MiSeq high-throughput sequencing. Plant materials were divided into group ME (without the addition of acetic acid), group AA (with acetic acid added at a dose of 0.3%), and group AB (with acetic acid added at a dose of 0.6%), and then changes in their chemical composition, fermentation quality, and microbial community diversity were analyzed over 30-day intervals. The content of dry matter (DM), water soluble carbohydrates (WSC), and crude protein (CP) were significantly (P < 0.05) in group AA higher than those in group ME after 60 days, while the content of acid detergent fiber (ADF) and acid detergent lignin (ADL) were significantly (P < 0.05) lower in group AA compared with those in group ME. The content of hemicellulose (HC), cellulose (CL), and holocellulose (HOC) were significantly higher in group AA compared with those in group ME (P < 0.05). The pH was significantly (P < 0.05) lower in group AB than that in group ME after both 30 and 60 days, while the content of lactic acid in this group increased significantly (P < 0.05) after 30 days, and then decreased significantly (P < 0.05) after 60 days, compared with that in group ME. As a result, the ratio of lactic acid to acetic acid (LA/AA), the ratio of lactic acid to total organic acid (LA/TOA), and the ratio of ammonia nitrogen to total nitrogen (AN/TN) in groups AA and AB were lower than those in group ME. The dominant bacterial taxa in these three groups were Proteobacteria and Firmicutes at the phylum level. At the genus level, the bacterial communities included Lactobacillus, Paralactobacillus, Enterobacter, Pediococcus, Flavobacterium, Chryseobacterium, Pedobacter, Sphingomonadaceae, and Erwinia, furthermore, Lactobacillus, Paralactobacillus, and Enterobacter were the dominant genera in the bacterial communities. The abundance of Enterobacter tended to decrease during the mixed storage period. The total abundance of lactic acid bacteria in groups AA and AB was higher than that in group ME, but the lactic acid bacterial diversity in groups AA and AB was lower than that in group ME, and the main lactic acid bacteria present were Lactobacillus, Paralactobacillus, and Pediococcus. In conclusion, at optimum proportions DMS and CW can be kept in mixed storage by ensiling while undergoing fermentation for 60 days while maintaining good quality. The storage quality was further improved by the addition of acetic acid, and we propose that the addition of acetic acid at a dose of 0.3% would be the most conducive to the preservation of the organic components of DMS and CW, such as DM, WSC, CP and HOC.

参考文献/References:

1. Mazzenga A, Gianesella M, Brscic M, Cozzi G. Feeding behaviour, diet digestibility, rumen fluid and metabolic parameters of beef cattle fed total mixed rations with a stepped substitution of wheat straw with maize silage [J]. Livest Sci, 2009, 122 (1): 16-23
2. Bhutto AW, Harijan K, Qureshi K, Bazmi AA, Bahadori A. Perspectives for the production of ethanol from lignocellulosic feedstock—a case study [J]. J Clean Prod, 2015, 95 (6): 184-193
3. Cie?lik M, Dach J, Lewicki A, Smurzyńska A, Janczak D, Pawlicka-Kaczorowska J, Boniecki P, Cyplik P, Czeka?a W, Jó?wiakowski K. Methane fermentation of the maize straw silage under meso- and thermophilic conditions [J]. Energy, 2016, 115 (2): 1495-1502
4. Liu EH, Wang CZ, Liu SY, Pan JX, Zhao KZ, Xu YT, Su ZY. Adsorption performance of activated carbon prepared from corn stalks [J]. Agric Sci Technol, 2016, 17 (10): 2221-2224, 2231
5. 杨道兰, 汪建旭, 冯炜弘, 张艳, 尹燕, 王永林. 花椰菜茎叶与玉米秸秆的混贮品质[J]. 草业科学, 2014, 31 (3): 551-557 [Yang DL, Wang JX, Feng WH, Zhang Y, Yin Y, Wang YL. Effects of broccoli stem sand leaves and maize straw mix-ensiling on silage quality [J]. Pratacult Sci, 2014, 31 (3): 551-557]
6. 任海伟, 赵拓, 李金平, 李雪雁, 徐娜, 王永刚, 王晓力, 高晓航. 玉米秸秆与废弃白菜的混贮品质及乳酸菌多样性研究[J]. 草业学报, 2016, 25 (1): 197-206 [Ren HW, Zhao T, LI JP, Li XY, Xu N, Wang YG, Wang XL, Gao XH. Quality and lactic acid bacteria of mixed corn stalk and cabbage waste silage [J]. Acta Pratacult Sin, 2016, 25 (1): 197-206]
7. 任海伟, 窦俊伟, 赵拓, 李雪雁, 李志忠, 李金平, 孙文斌, 黄娟娟. 添加剂对玉米秸秆和莴笋叶混贮品质的影响[J]. 草业学报, 2016, 25 (10): 142-152 [Ren HW, Dou JW, Zhao T, Li XY, Li ZZ, Li JP, Sun WB, Huang JJ. Effects of additives on the mixed silage quality of corn stover and asparagus [J]. Acta Pratacult Sin, 2016, 25 (10): 142-152]
8. 李娟, 王文丽, 赵旭. 马铃薯渣和玉米秸秆混合发酵产蛋白质饲料研究[J]. 中国饲料, 2013 (11): 40-42 [Li J, Wang WL, Zhao X. Study on protein feed produced by mixed fermentation of potato residue and corn stalk [J]. China Feed, 2013 (11): 40-42]
9. 李红宇, 许丽, 方美琪, 刘文娟, 王楠. 黄贮玉米秸秆中玉米浆添加水平对其发酵品质主要指标的影响[J]. 东北农业大学学报, 2013, 44 (9): 137-143 [Li HY, Xu L, Fang MQ, Liu WJ, Wang N. Effect of addition level for corn steep liquor on main index of fermentation quality in yellow com stover silage [J]. J Northeast Agric Univ, 2013, 44 (9): 137-143]
10. 张晓庆, 李鹏, 郑琛, 金艳梅. 添加甲酸对麻叶荨麻青贮品质的影响[J]. 草地学报, 2013, 21 (3): 618-621 [Zhang XQ, Li P, Zheng C, Jin YM. Effect of formic acid on silage quality of hempleaf nettle (Urtica cannabina) [J]. Acta Agestia Sin, 2013, 21 (3): 618-621]
11. 郭艳萍, 玉柱, 顾雪莹, 孙启忠, 朝克图. 不同添加剂对高粱青贮质量的影响[J]. 草地学报, 2010, 18 (6): 875-879 [Guo YP, Yu Z, Gu XY, Sun QZ, Chao KT. Effects of different additives on silage quality of sorghum [J]. Acta Agrestia Sin, 2010, 18 (6): 875-879]
12. Schmidt RJ, Jr Kung L. The effects of Lactobacillus buchneri with or without a homolactic bacterium on the fermentation and aerobic stability of corn silages made at different locations [J]. J Dairy Sci, 2010, 93 (4): 1616-1624
13. Oleskowicz-Popiel P, Thomsen AB, Schmidt JE. Ensiling—Wet-storage method for lignocellulosic biomass for bioethanol production [J]. Biomass Bioenergy, 2011, 35 (5): 2087-2092
14. 陶莲, 刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报, 2016, 28 (1): 198-207 [Tao L, Diao QY. Effects of ensiling on Quality and bacteria composition of corn stalk [J]. Chin J Anim Nutr, 2016, 28 (1): 198-207]
15. Ni KK, Wang FF, Zhu BG, Yang JX, Zhou GA, Pan Y, Tao Y. Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage [J]. Bioresour Technol, 2017, 238: 706-715
16. 胡宗福, 常杰, 萨仁呼, 王思珍, 牛化欣. 基于宏基因组学技术检测全株玉米青贮期间和暴露空气后的微生物多样性[J]. 动物营养学报, 2017, 29 (10): 1-11 [Hu ZF, Chang J, Sarnhu, Wang SZ, Niu HX. Microbial diversity of whole-plant maize during ensilage and after air exposure analyzed by metagenomics technology [J]. Chin J Anim Nutr, 2017, 29 (10): 1-11]
17. Li LH, Sun YM, Yuan ZH, Kong XY, Wao Y, Yang LG, Zhang Y, Li D. Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of many flower silvergrass [J]. Bioresour Technol, 2015, 189 (10): 334-340
18. 许颖, 马德胜, 宋文枫, 魏小芳. 16S rDNA高通量测序技术分析油藏微生物多样性[J]. 应用与环境生物学报, 2016, 22 (3): 409-414 [Xu Y, Ma DS, Song WF, Wei XF. 16S rDNA-assisted high-throughput sequencing analysis of microbial diversity in oil reservoirs [J]. Chin J Appl Environ Biol, 2016, 22 (3): 409-414]
19. 许艳蕊, 方志军, 卢晓平, 郝鲁江. 基于高通量测序技术分析使它隆对玉米土壤细菌多样性的影响[J]. 微生物学报, 2017, 57 (7): 985-993 [Xu YR, Fang ZJ, Lu XP, Hao LJ. Effects of starane on maize soil bacterial diversity analyzed by high-throughput sequencing technology [J]. Acta Microbiol Sin, 2017, 57 (7): 985-993]
20. 邱小燕, 原现军, 郭刚, 闻爱友, 余成群, 巴桑, 邵涛. 添加糖蜜和乙酸对西藏发酵全混合日粮青贮发酵品质及有氧稳定性影响[J]. 草业学报, 2014, 23 (6): 111-118 [Qiu XY, Yuan XJ, Guo G, Wen AY, Yu CQ,Ba S, Shao T. Effects of molasses and acetic acid on fermentation and aerobic stability of total mixed ration silage in Tibet [J]. Acta Pratacult Sin, 2014, 23 (6): 111-118]
21. 李君风, 孙肖慧, 原现军, 郭刚, 肖慎华, 巴桑, 余成群, 邵涛. 添加乙酸对西藏燕麦和紫花苜蓿混合青贮发酵品质和有氧稳定性的影响[J]. 草业学报, 2014, 23 (5): 271-278 [Li JF, Sun XH, Yuan XJ, Guo G, Xiao SH, Ba S, Yu CQ, Shao T. Effect of adding acetic acid on fermentation quality and aerobic stability of mixed oat and alfalfa silage in Tibet [J]. Acta Pratacult Sin, 2014, 23 (5): 271-278]
22. 荣辉, 余成群, 陈杰, 下条雅敬, 邵涛. 添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响[J]. 草业学报, 2013, 22 (3): 108-115 [Rong H, Yu CQ, Chen J, Shimojo M, Shao T. Effects of FJLB, LAB and glucose addition on fermentation quality of napier grass (Pennisetum purpureum) silage [J]. Acta Pratacult Sin, 2013, 22 (3): 108-115]
23. 徐炜, 师尚礼, 祁娟, 张文渝, 王兴中, 王新强, 王皎. 不同添加剂对夏河县紫花苜蓿青贮品质的影响[J]. 草地学报, 2014, 22 (2): 414-419 [Xu W, Shi SL, Qi J, Zhang WY, Wang XZ, Wang XQ, Wang J. Effects of different additives on the quality of alfalfa silage in xiahe [J]. Acta Agestia Sin, 2014, 22 (2): 414-419]
24. 张亚格, 字学娟, 李茂, 周汉林. 有机酸对柱花草青贮品质和营养成分的影响[J]. 动物营养学报, 2016, 28 (5): 1609-1614 [Zhang YG, Zi XJ, Li M, Zhou HL. Effects of organic acids on quality and nutrient composition of stylosanthes guianensis silage [J]. Chin J Anim Nutr, 2016, 28 (5): 1609-1614]
25. 许庆方, 张翔, 崔志文, 李志强. 不同添加剂对全株玉米青贮品质的影响[J]. 草地学报, 2009, 17 (2): 157-161 [Xu QF, Zhang X, Cui ZW, Li ZQ. Effect of additives on the quality of whole-crop corn silage [J]. Acta Agestia Sin, 2009, 17 (2): 157-161]
26. 张利亚, 孙优善, 吕学斌, 张书廷. 不同青贮条件下秸秆组分的变化特性及水解效果[J]. 安全与环境学报, 2016, 16 (1): 258-262 [Zhang LY, Sun YS, Lv XB, Zhang ST. Variable characteristics of the stalk components and the hydrolysis effect under different silage conditions [J]. J Saf Environ, 2016, 16 (1): 258-262]
27. Guo XL, Zhou H, Yu Z, Zhang Y. Changes in the distribution of nitrogen and plant enzymatic activity during ensilage of lucerne treated with different additives [J]. Grass For Sci, 2007, 62 (1): 35-43
28. 韩立英, 玉柱. 3种乳酸菌制剂对苜蓿和羊草的青贮效果[J]. 草业科学, 2009, 26 (2): 66-71 [Han LY, Yu Z. Effect of adding three different lactobacillus additives on Medicago sativa and Leymus chinensis silage qualities [J]. Pratacult Sci, 2009, 26 (2): 66-71]
29. 孟庆翔, 杨军香. 全株玉米青贮制作与质量评价[M]. 北京: 中国农业科学技术出版社, 2016 [Meng QX, Yang JX. Production and quality evaluation of whole plant corn silage [M]. Beijing: China Agricultural Science & Technolegy Press, 2016]
30. 傅彤. 微生物接种剂对玉米青贮饲料发酵进程及其品质的影响[D]. 北京: 中国农业科学院, 2005 [Fu T. The effects of microbial inoculants on the fermentation process and quality of corn silage [D]. Beijing: Chinese Academy of Agricultural Science, 2005]
31. 唐振华, 侯宇, 邹彩霞, 夏中生, 韦升菊, 梁辛, 李丽莉, 梁贤威. 甲酸、乙酸对甘蔗尾青贮发酵、营养品质和有氧稳定的影响[J]. 饲料工业, 2015, 36 (19): 15-18 [Tang ZH, Hou Y, Zou CX, Xia ZS, Wei SJ, Liang X, Li LL, Liang XW. Effect of formic acid and acetic acid on the quality and aerobic stability of sugarcane tops silage [J]. Feed Ind, 2015, 36 (19): 15-18]
32. Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions [J]. Appl Environ Microbiol, 2003, 69 (1): 562-567
33. 王保平, 董晓燕, 董宽虎, 许庆方. 有机酸对全株玉米青贮有氧稳定性的影响[J]. 草地学报, 2013, 21 (5): 991-997 [Wang BP, Dong XY, Dong KH, Xu QF. Effects of organic acid on the aerobic stability of whole-crop corn silage [J]. Acta Agestia Sin, 2013, 21 (5): 991-997]
34. 侯建建, 白春生, 张庆, 玉柱. 单一和复合乳酸茵添加水平对苜蓿青贮营养品质及蛋白组分的影响[J]. 草业科学, 2016, 33 (10): 2119-2125 [Hou JJ, Bai CS, Zhang Q, Yu Z. Effects of different additives amount of single and multiple lactic acid bacteria on the silage quality and protein fractions of alfalfa [J]. Pratacult Sci, 2016, 33 (10): 2119-2125]
35. 乔江涛, 郭荣波, 袁宪正, 师晓爽, 许晓辉, 范晓蕾, 邱艳玲. 玉米秸秆厌氧降解复合菌系的微生物群落结构[J]. 环境科学, 2013, 34 (4): 1531-1539 [Qiao JT, Guo RB, Yuan XZ, Shi XS, Xu XH, Fan XL, Qiu YL. Phylogenetic analysis of methanogenic corn stalk degrading microbial communities [J]. Environ Sci, 2013, 34 (4): 1531-1539]
36. Dunière L, Sindou J, Chaucheyras-Durand F, Chevallier I, Thévenot-Sergentet D. Silage processing and strategies to prevent persistence of undesirable microorganisms [J]. Anim Feed Sci Technol, 2013, 182 (1-4): 1-15
37. Ogunade IM, Jiang Y, Kim DH, Pech Cervantes AA, Arriola KG, Vyas D, Weinberg ZG, Jeong KC, Adesogan AT. Fate of Escherichia coli O157: H7 and bacterial diversity in corn silage contaminated with the pathogen and treated with chemical or microbial additives [J]. J Dairy Sci, 2016, 100 (3): 1780-1794
38. 席琳乔. 棉花秸秆木质纤维素降解及其青贮复合菌系的筛选和协同机理研究[D]. 哈尔滨: 东北农业大学, 2014 [Xi LQ. Screening complex degradation strains of cotton straw lignocelluloses and silages strains and researching [D]. Harbin: Northeastern Agricultural University, 2014]
39. 华彬彬. 分解木质纤维素复合菌系的形成机理及应用[D]. 北京: 中国农业大学, 2017 [Hua BB. Formation mechanism and application of lignocellulose degradation microbial community [D]. Beijing: China Agricultural University, 2017]
40. 韩吉雨, 侯先志, 杨凯, 赵子夫, 郭天龙, 王丽, 王海荣. PCR-DGGE方法分析内蒙古不同地区青贮玉米中乳酸菌群多样性[J]. 动物营养学报, 2009, 21 (6): 974-981 [Han SY, Hou XZ, Yang K, Zhao ZF, Wang L, Wang HR. Study of Lactobacillus population diversity in corn silage by PCR-DGGE in different regions of inner mongolia [J]. Chin J Anim Nutr, 2009, 21 (6): 974-981]
41. 张志飞, 穆麟, 文昭竹, 龚梨霞, 魏仲珊, 李志才. 不同添加剂对紫花苜蓿裹包青贮品质及菌群构成的影响[J]. 中国草地学报, 2017, 39 (4): 54-59 [Zhang ZF, Mu L, Wen ZZ, Gong LX, Wei ZS, Li ZC. Effects of different additives on quality and bacterial community composition of alfalfa wrapped silage [J]. Chin J Grassl, 2017, 39 (4): 54-59]
42. 包慧芳, 王炜, 王宁, 詹发强, 赵忠润. 玉米秸秆青贮过程中优势细菌多样性分析[J]. 微生物学通报, 2010, 37 (8): 1247-1251 [Bao HF, Wang W, Wang N, Zhan FQ, Zhao ZR. Diversity of predominant bacteria during ensiling [J]. Microbiol China, 2010, 37 (8): 1247-1251]
43. 陶雅, 李峰, 高凤芹, 徐春城, 孙启忠. 籽粒苋与青贮玉米混贮品质及微生物特性研究[J]. 草业学报, 2016, 25 (12): 119-129 [Tao Y, Li F, Gao FQ, Xu CC, Sun QZ. Fermentation quality and microbial characteristics of Amaranthus hypochondriacus corn mixed silage [J]. Acta Pratacult Sin, 2016, 25 (12): 119-129]
44.

相似文献/References:

[1]任海伟,刘菲菲,李梦玉,等.玉米秸秆和白菜废弃物在不同贮存条件下的微生物群落[J].应用与环境生物学报,2018,24(02):281.[doi:10.19675/j.cnki.1006-687x.2017.05035]
 REN Haiwei,LIU Feifei,LI Mengyu,et al.Microbial community of maize straw and cabbage waste under different storage conditions[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):281.[doi:10.19675/j.cnki.1006-687x.2017.05035]
[2]任海伟,刘菲菲,王莉,等.纤维素酶对干玉米秸秆/白菜废弃物混贮品质的影响及细菌群落解析[J].应用与环境生物学报,2018,24(05):1097.[doi:10.19675/j.cnki.1006-687x.2017.12051]
 REN Haiwei,LIU Feifei,et al.Effects of cellulase on the mixed silage quality of dried maize straw and cabbage waste and an analysis of its bacterial community[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1097.[doi:10.19675/j.cnki.1006-687x.2017.12051]
[3]任海伟 冯银萍 刘 通 王 昱 李志忠* 裴佳雯 王 蕾 李盼盼.温度对干玉米秸秆与废弃白菜混贮发酵品质的影响和微生物菌群解析[J].应用与环境生物学报,2019,25(03):1.[doi:10.19675/j.cnki.1006-687x.2018.09024]
 Ren Haiwei,Feng Yingping,Liu Tong,et al.Effects of temperature o n the mixed storage fermentation quality of dry corn stalk and cabbage waste and its microbial community analysis[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1.[doi:10.19675/j.cnki.1006-687x.2018.09024]

更新日期/Last Update: 2018-10-25