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[1]黄艳蒙,张爱平,曹沁,等.甲基营养型芽孢杆菌H0对羽毛产寡肽的降解特征[J].应用与环境生物学报,2018,24(06):1312-1317.[doi:10.19675/j.cnki.1006-687x.2018.01047]
 HUANG Yanmeng,et al..The oligopeptides production by the feather-degrading bacterium Bacillus methylotrophicus H0[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1312-1317.[doi:10.19675/j.cnki.1006-687x.2018.01047]
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甲基营养型芽孢杆菌H0对羽毛产寡肽的降解特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1312-1317
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
The oligopeptides production by the feather-degrading bacterium Bacillus methylotrophicus H0
作者:
黄艳蒙 张爱平 曹沁 李东 刘晓风
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049
Author(s):
HUANG Yanmeng et al.
HUANG Yanmeng1, 2, ZHANG Aiping1, 2, CAO Qin1, LI Dong1** & LIU Xiaofeng1** 1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
羽毛角蛋白分离鉴定甲基营养型芽孢杆菌生物降解寡肽
Keywords:
feather keratin isolation and identification Bacillus methylotrophicus biodegradation oligopeptide
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2018.01047
摘要:
寡肽具有较高的营养价值和多种生理功能,筛选高产寡肽的羽毛降解菌株,并将其用于羽毛寡肽的生产,对高效利用废弃羽毛资源、提高产品附加值具有重要意义. 以寡肽产率为评价指标,从16株羽毛降解菌中分离到一株高产寡肽的羽毛降解菌H0,通过形态观察、生理鉴定和系统发育分析,初步鉴定菌株H0为甲基营养型芽孢杆菌(Bacillus methylotrophicus). 对其降解羽毛产寡肽特性和羽毛寡肽特征进行初步探究,发现该菌在最佳初始pH(11)和最佳温度(40 ℃)下发酵72 h,羽毛几乎完全降解,寡肽产率达38.19%(占可溶性总肽的67.53%),同时检测到11.11%的游离氨基酸. 液质联用(LC-MS/MS)分析结果表明所产寡肽的分子量(Mr)主要分布在1 300以下,且主要是由5-10个氨基酸组成的短肽,富含支链氨基酸,推测其是羽毛降解液呈现抗氧化活性的主要原因. 本研究表明甲基营养型芽孢杆菌H0具有较强的降解羽毛产寡肽能力,可为羽毛寡肽产品的开发提供优质微生物资源和科学依据. (图8 表2 参22)
Abstract:
Due to the high nutritive value of oligopeptides and the waste of feather resources, this study aimed at screening efficient strains of bacteria able to rapidly degrade feathers and produce large quantities of value-added oligopeptides. In order to assess the potential yield of oligopeptides, the promising strain H0 was selected from 16 feather-degrading microorganisms. To identify the strain, we analyzed the morphological and physiological characteristics of different strains, and carried out a gene sequence analysis of their 16S rRNAs. A single factor experiment was used to promote feather degradation and oligopeptide production, and the characteristics of the oligopeptides produced were also analyzed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). The strain was identified as Bacillus methylotrophicus. The optimal initial pH and temperature for oligopeptide production were 11 and 40 ℃, respectively. After 72 h of fermentation under these optimal conditions, the feathers were almost completely degraded, with a 38.19% of oligopeptides (accounting for 67.53% of the total soluble peptides) and a 11.11% of free amino acids produced. LC-MS/MS analysis indicated that the oligopeptides were mainly short peptides containing 5-10 amino acids, with a molecular mass (Mr) of less than 1 300. Moreover, the peptides were abundant in branched-chain amino acids, that might be responsible for the antioxidant property of the feather hydrolysate. Our results demonstrate the great capability of B. methylotrophicus H0 in feather degradation and oligopeptide production. This research provides a high-quality microorganism resource, and the scientific basis for the development of feather-derived oligopeptide products.

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更新日期/Last Update: 2018-12-25