|本期目录/Table of Contents|

[1]张晓梅,窦文芳,许泓瑜,等.一株产脂肪酶嗜盐菌的鉴定及耐盐机理[J].应用与环境生物学报,2010,16(01):100-103.[doi:10.3724/SP.J.1145.2010.00100]
 ZHANG Xiaomei,DOU Wenfang,XU Hongyu,et al.Identification of a Lipase Producing Halophilic Strain and Its Halophilic Mechanism[J].Chinese Journal of Applied & Environmental Biology,2010,16(01):100-103.[doi:10.3724/SP.J.1145.2010.00100]
点击复制

一株产脂肪酶嗜盐菌的鉴定及耐盐机理()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年01期
页码:
100-103
栏目:
研究论文
出版日期:
2010-02-25

文章信息/Info

Title:
Identification of a Lipase Producing Halophilic Strain and Its Halophilic Mechanism
作者:
张晓梅窦文芳许泓瑜许正宏
(1江南大学医药学院制药工程研究室,2江南大学工业生物技术教育部重点实验室 无锡 214122)
Author(s):
ZHANG Xiaomei DOU Wenfang XU Hongyu XU Zhenghong
(1Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, Jiangsu, China)
(2Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China)
关键词:
菌种鉴定Halorubrum aidingense脂肪酶极端嗜盐菌四氢嘧啶
Keywords:
strain identification Halorubrum aidingense lipase extreme halophile ectoine
分类号:
Q939.97 : TQ925.6
DOI:
10.3724/SP.J.1145.2010.00100
文献标志码:
A
摘要:
从采自内蒙古锡林浩特地区盐湖的样品中筛选到产脂肪酶嗜盐菌JNPH-3,该菌株可在含有1.5~5 mol/L NaCl的培养基上生长,最适生长温度45 ℃,最适pH 7~9.5. 经形态学分析、生理生化实验及16S rDNA鉴定,JNPH-3菌株为Halorubrum aidingense,属于极端嗜盐菌. JNPH-3菌株在生长过程中专一性依赖Na+,对Cl-依赖性较小,K+浓度变化对菌体生长无明显影响. 高效液相色谱分析表明,JNPH-3菌株在液体复合培养基中能够产生四氢嘧啶,且与环境中盐浓度呈负相关,说明JNPH-3体内除存在产生四氢嘧啶的机制来维持渗透平衡,还有其它机制来维持细胞正常生长. 图3 表3 参14
Abstract:
A novel halophile strain producing lipase, named as JNPH-3, was screened from the samples isolated from hypersaline lakes in Inner Mongolia. It could grow in the medium containing1.5~5 mol/L NaCl, and optimally at temperature of 45 ℃ and pH of 7~9.5. It was identified as Halorubrum aidingense, belonging to the group of extreme halophiles, by its morphological, physiological and biochemical characters, and 16S rDNA sequences. The growth of JNPH-3 specifically depended on anion Na+ , but other anion and cation ions such as Cl-, Br-, I-, K+, Li+ had no obvious effect on its growth. It was also found that JNPH-3 could produce ectoine in the complex medium. However, the yield of ectoine was negatively related with the concentration of environmental NaCl, which was not consistent with the well-known mechanism of halophile strain, suggesting that JNPH-3 had another mechanism to support its growth, besides the mechanism of producing ectoine to keep its osmotic equilibrium. Fig 3, Tab 3, Ref 14

参考文献/References:

1 Lü AJ (吕爱军), Yi JM (尹建美), Hu XC (胡秀彩), Zhang P (张鹏), Hu B (胡斌), Wen HY (温洪宇). Characteristics of extreme falophile and its application prospects. J Microbiol (微生物学杂志), 2005, 25 (2): 65~68
2 Frutos C, Marhuenda-Egea, María José Bonete. Extreme halophilic enzymes in organic solvents. Curr Opin Biotechnol, 2002, 13: 385~389
3 Sekhon A, Dahiya N, Tiwari RP, Hoondal GS. Properties of a thermostable extracellular lipase from Bacillus megaterium AKG-1. J Basic Microbiol, 2005, 45 (2): 147~154
4 Ghosh M, Bhattacharyya D. Lipase-catalyzed synthesis of hydroxy stearates and their properties. J Am Oil Chem Soc, 1998, 75 (8): 1057~1059
5 Gupta R, Gupta N, Rathi P. Bacterial lipases: An overview of production, purification and biochemical properties. Appl Microbiol & Biotechnol, 2004, 64: 763~781
6 Zhang B (张搏), Yang JK (杨江科), Yan YJ (闫云君). Research progress on pseudomonas lipases. Biotechnol Bull (生物技术通报), 2007, 2: 52~57
7 Jiang YM (蒋咏梅), Zhang WX (章文贤), Zhou XL (周晓兰), Shi QQ (施巧琴). Construction of library of lipase-proudcing strains in extreme enviroments and studies of lipase. Biotechnology (生物技术), 2003, 13 (5): 9~10
8 Sambrook J, Russell DW. Molecular Cloning. Translated by Huang PT (黄培堂). 3rd ed. Beijing, China (北京): Science Press, 2002
9 Dong XZ (东秀珠), Cai MY (蔡妙英). Handbook of Common Bacteria Systematic Identify. Beijing, China (北京): Science Press, 2001
10 Zhou YH (周月慧), Zhang LH (张苓花), Wang F (王飞), Yong-Tian JY (永田进一), Wang YJ (王运吉). Study on the ectoine synthesis with moderately halobacterium-SL21. J HeNan Univ Technology (河南工业大学学报), 2006, 27 (2): 81~85
11 Heng LC, Dilbr T, Pei JZ. Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov, isolated from two salt lakes in Xinjiang. Intern J Syst & Evol Microbiol, 2006, 56: 1631~1634
12 Cao JW (曹军卫), Shen P (沈萍), Li ZY (李朝阳). Halophilic Microorganisms. Wuhan, China (武汉): Wuhan University Press, 2004. 19~23
13 Ren PG (任培根), Zhou PJ (周培瑾). Reseach progress of moderately halophilic Eubacteria. Acta Microbiol Sin (微生物学报), 2003, 43 (3): 427~431
14 He J (何健), WangT (汪婷), Sun JQ (孙纪全). Isolation and characteristic of a moderately halophilic bacterium accumulated ectoine as main compatible solute. Acta Microbiol Sin (微生物学报), 2005, 45 (6): 900~904

相似文献/References:

[1]陈哈斯额尔敦,余志晟,林文芳,等.一株染料脱色酵母菌的鉴定及脱色特性[J].应用与环境生物学报,2011,17(02):247.[doi:10.3724/SP.J.1145.2011.00247]
 CHEN Hasierdun,YU Zhisheng,LIN Wenfang,et al.Identification of a New Isolated Yeast and Its Decolorization on Dyes[J].Chinese Journal of Applied & Environmental Biology,2011,17(01):247.[doi:10.3724/SP.J.1145.2011.00247]
[2]马海林,邢尚军,刘方春,等.冬枣(Ziziphus jujube Mill.)根际3株促生细菌的筛选与鉴定[J].应用与环境生物学报,2013,19(04):650.[doi:10.3724/SP.J.1145.2013.00650]
 MA Hailin,XING Shangjun,LIU Fangchun,et al.Screening and Identification of Three Plant Growth-promoting Rhizobacteria from Winter Jujube (Ziziphus jujube Mill.) Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):650.[doi:10.3724/SP.J.1145.2013.00650]

备注/Memo

备注/Memo:
江苏省博士后基金(No. SY20061019),国家“973”项目(No. 2007CB707804)和国家“863”重点项目(No. 2006AA020104)资助
更新日期/Last Update: 2010-02-09