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[1]王柳红,奚慧,黄兴华,等.城市垃圾填埋场抗生素抗性基因的污染特征[J].应用与环境生物学报,2019,25(02):333-338.[doi:10.19675/j.cnki.1006-687x.2018.06039]
 WANG Liuhong,XI Hui,HUANG Xinghua,et al.Characteristics of antibiotic resistance genes in municipal solid waste landfill[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):333-338.[doi:10.19675/j.cnki.1006-687x.2018.06039]
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城市垃圾填埋场抗生素抗性基因的污染特征
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
333-338
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Characteristics of antibiotic resistance genes in municipal solid waste landfill
作者:
王柳红奚慧黄兴华孙金昭谢冰
华东师范大学生态与环境科学学院,上海城市生态过程与生态恢复重点实验室 上海 200241
Author(s):
WANG Liuhong XI Hui HUANG Xinghua SUN Jinzhao & XIE Bing**
School of Ecological and Environment Science, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China
关键词:
城市垃圾填埋场抗生素抗性基因固体垃圾渗滤液季节
Keywords:
landfill antibiotic resistance gene solid waste leachate season
分类号:
X502
DOI:
10.19675/j.cnki.1006-687x.2018.06039
摘要:
抗生素抗性基因是一种新型的环境污染物,为探究抗生素抗性基因在垃圾填埋场的污染特征,采集上海老港垃圾填埋场中固体垃圾和渗滤液样品,采用实时荧光定量PCR技术检测磺胺类抗生素抗性基因(sul1、sul2)、四环素类抗性基因(tetM、tetQ)、氨基糖苷类抗性基因(strB、aadA1)、大环内酯类抗生素抗性基因(ermB、mefA)、多重抗性基因(mexF)及I类整合子(intl1)等6类目标基因的丰度. 结果显示,6类目标基因均在固体垃圾和渗滤液中检测到,丰度分别介于102-106、103-107/ng,且多重抗性基因、氨基糖苷类及磺胺类抗生素抗性基因检出丰度较高. 在填埋场固体垃圾中,部分目标基因在1.5 m深处的丰度高于0.5 m深处;在渗滤液中,目标基因丰度和呈现老龄渗滤液大于新鲜渗滤液,部分目标基因在秋季的丰度大于春季. 上述结果说明垃圾填埋场是抗生素抗性基因潜在的储存库,目标基因的丰度在垃圾填埋场中存在时空差异. (图8 表2 参41)
Abstract:
Antibiotic resistance genes (ARGs) are known to be emerging environmental contaminants and pose a threat to public health. Solid waste and leachate were collected from the Shanghai Laogang landfills to study the pollution characteristics of the ARGs. The Real-time Quantitative PCR method was used to measure the abundance of six types of target genes, including the sulfonamide antibiotic resistance genes (sul1, sul2), tetracycline resistance genes (tetM, tetQ), aminoglycoside resistance genes (strB, aadA1), macrolide antibiotic resistance genes (ermB, mefA), multiplex resistance gene (mexF), and class I integron gene (intl1). The obtained results showed that the six types of target genes were present in both solid waste and leachate. The concentration of the target genes in solid waste and leachate ranged from 102-106/ng and 103-107/ng, respectively. Moreover, the concentration of the multi-resistance genes, aminoglycosides, and sulfonamide antibiotic resistance genes was higher than that of the other genes. The concentration of some target genes in solid waste collected at a depth of 1.5 m was higher than that collected at a depth of 0.5 m, and the total quantity of target genes in the mature leachate was higher than that in the fresh leachate. Additionally, the contribution of some target genes was higher in autumn than in spring. The results demonstrate that the municipal solid waste landfill is a potential reservoir of ARGs and the concentration of target genes demonstrate a spatiotemporal variation.

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更新日期/Last Update: 2019-04-25