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[1]韩雪花,胡瀚文,罗禹,等.白及内生菌Penicillium oxalicum的化学成分[J].应用与环境生物学报,2019,25(02):438-444.[doi:10.19675/j.cnki.1006-687x.2018.07020]
 HAN Xuehua,HU Hanwen,LUO Yu** & DENG Yun**.Chemical constituents of Penicillium oxalicum, an endophytic fungus isolated from Bletilla striata (Thunb.) Reichb. f.[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):438-444.[doi:10.19675/j.cnki.1006-687x.2018.07020]
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白及内生菌Penicillium oxalicum的化学成分
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
438-444
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Chemical constituents of Penicillium oxalicum, an endophytic fungus isolated from Bletilla striata (Thunb.) Reichb. f.
作者:
韩雪花胡瀚文罗禹邓赟
1成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室省部共建国家重点实验室培育基地 成都 611137 2西南医科大学药学院 泸州 646000
Author(s):
HAN Xuehua1 HU Hanwen1 LUO Yu2** & DENG Yun1**
1 Key Laboratory of Standardization of Chinese Herbal Medicine of Ministry of Education, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medical Resources Co-founded by Sichuan Province and Minrstry of Science and Technology, Pharmacy Department, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China 2 School of Pharmacy, Southwest Medical University, Luzhou 646000, China
关键词:
白及草酸青霉菌化学成分分离鉴定
Keywords:
Bletilla striata (Thunb.) Reichb. f. Penicillium oxalicum chemical constituent isolation and elucidation
分类号:
R914
DOI:
10.19675/j.cnki.1006-687x.2018.07020
摘要:
对从中药白及新鲜药材中分离得到的一株内生菌草酸青霉菌Penicillium oxalicum进行固体发酵40 d后得到发酵产物,将发酵产物采用75%乙醇渗漉提取后,用乙酸乙酯萃取,并利用硅胶柱色谱、Sephadex LH-20凝胶、HPLC等方法进行成分分离. 从中分离得到15个单体化合物,经质谱、核磁共振等波谱方法鉴定为α-亚麻酸甲酯(1)、亚油酸甲酯(2)、benzoic acid(3)、4-hydroxybenzaldehyde(4)、ganodermasides D(5)、dankasterone A(6)、calvasterol B(7)、cyclo-(Pro-Phe)(8)、penioxalicin(9)、5α-麦角甾-7,22-二烯-3β,5,6β-三醇(10)、cis-4-hydroxyscytalone(11)、3,4-dihydroxyphenylacetic acid methyl ester(12)、protocatechuic acid(13)、cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(14)、cyclo-L-(4-hydroxyprolinyl)-L-leucine(15). 其中化合物1以及4-15均为首次从P. oxalicum中分离得到. 本研究结果可促进白及及其内生菌资源的开发利用.(图1 参32)
Abstract:
To study the isolation and elucidation of chemical constituents of Penicillium oxalicum, an endophytic fungus was isolated from Bletilla striata (Thunb.) Reichb. f. After solid state fermentation for 40 days, secondary metabolites were percolated by ethanolwater (75:25, V/V), extracted with ethyl acetate, and purified by column chromatography methods including silica gel, Sephadex LH-20, and high-performance liquid chromatography. A total of 15 compounds were isolated. Their structures were elucidated through extensive spectroscopic analyses as follows: α-methyl linolenic acid (1), methyl linoleate (2), benzoic acid (3), 4-hydroxybenzaldehyde (4), ganodermasides D (5), dankasterone A (6), calvasterol B (7), cyclo-(Pro-Phe) (8), penioxalicin (9), 5α-ergoside-7,22-diene-3β,5,6β-triol (10), cis-4-hydroxyscytalone (11), 3,4-dihydroxyphenylacetic acid methyl ester (12), protocatechuic acid (13), cyclo-(4-S-hydroxy-R-proline-R-isoleucine) (14), and cyclo-L-(4-hydroxyprolinyl)-L-leucine (15). Among these, compounds 1 and 4-15 were isolated from P. oxalicum for the first time.

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