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[1]刘海霞,曹曦跃,吴海珍,等.隐球酵母(Cryptococcus podzolicus)Zwy-2-3循环利用油脂发酵废弃细胞产油[J].应用与环境生物学报,2017,23(06):979-983.[doi:10.3724/SP.J.1145.2017.03025]
 LIU Haixia,CAO Xiyue,WU Haizhen,et al.Cryptococcus podzolicus Zwy-2-3 recycling fermentation waste for the microbial lipid production[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):979-983.[doi:10.3724/SP.J.1145.2017.03025]
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隐球酵母(Cryptococcus podzolicus)Zwy-2-3循环利用油脂发酵废弃细胞产油()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
979-983
栏目:
微生物资源发掘与生物合成专栏论文
出版日期:
2017-12-25

文章信息/Info

Title:
Cryptococcus podzolicus Zwy-2-3 recycling fermentation waste for the microbial lipid production
作者:
刘海霞 曹曦跃 吴海珍 陈国成 张倩 曾杰 王雨昊 徐辉 乔代蓉 曹毅
1四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065 2东北农业大学食品学院 哈尔滨 150036
Author(s):
LIU Haixia1 CAO Xiyue2 WU Haizhen1 CHEN Guocheng1 ZHANG Qian1 ZENG Jie1 WANG Yuhao1 XU Hui1 QIAO Dairong** & CAO Yi**
1 Microbiology and Metabolic Engineering of Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China 2 College of Food Science, Northeast Agricultural University, Harbin 150036, China
关键词:
栎类淀粉隐球酵母Zwy-2-3同步糖化发酵氮源循环
Keywords:
acron starch Cryptococcus podzolicus Zwy-2-3 simultaneous saccharification and fermentation recycle nitrogen resource
分类号:
Q939.97
DOI:
10.3724/SP.J.1145.2017.03025
摘要:
为提高资源利用效率,降低微生物油脂发酵成本,解决微生物油脂发酵中废弃酵母细胞和发酵废液处理排放的问题,研究隐球酵母(Cryptococcus podzolicus)Zwy-2-3利用栎类淀粉发酵产油情况,并探讨发酵废液和废弃酵母细胞酶解液作为氮源的循环利用. 结果显示,以葡萄糖60 g/L和总氮0.18 g/L的废弃酵母细胞酶解液发酵时,循环3次其油脂产量分别达到6.79 g/L、6.66 g/L、6.72 g/L,均高于对照组;而将发酵废液回收用作废弃酵母细胞酶解的缓冲液时,其生物量、油脂产量同对照组相当;将该方法应用于栎类淀粉水解液同步糖化发酵产油脂的实验,循环3次后其生物量、油脂产量分别为13.04 g/L、7.13 g/L,比对照组提高了9.85%、10.03%,且3次循环的油脂含量较为稳定. 油脂组分分析结果显示,菌株Zwy-2-3利用栎类淀粉同步糖化和废弃细胞循环酶解液发酵生产的微生物油脂不饱和脂肪酸的含量达到93%以上,与植物油组成相似. 综上,酶解废弃酵母细胞可有效应用于酵母产油发酵,可为非粮淀粉生产的微生物油脂应用于生物柴油生产奠定基础. (图3 表2 参21)
Abstract:
To improve the efficacy of resources, reduce the costs of microbial lipid production, and solve the problem of disposal of the spent cell mass and spent water, it is imperative to recycle resources, including spent cell mass, mineral nutrients, and spent water. In this study, lipid production by the oleaginous yeast Cryptococcus podzolicus Zwy-2-3 was used as a model system to demonstrate the recycling of an enzymatic solution of spent cell mass and spent water as a nitrogen resource for microbial lipid fermentation. It was found that when serial repitching experiments were performed with 60 g/L glucose and spent cell mass hydrolysates alone as a nitrogen resource, the fermentation produced 6.79 g/L, 6.66 g/L, and 6.72 g/L lipid yield in three successive cycles, which was higher than that in the control. In contrast, biomass and the lipid yield remained almost constant when spent water was also recycled. The method was applied to the simultaneous saccharification and fermentation of an acorn starch hydrolysate, and after three cycles, the biomass and lipid yield were 13.04 g/L and 7.13 g/L, the growth rates were 9.85% and 10.03%, respectively, and the lipid content of the three cycles was stable. Our results show efficient recycling of lipid production waste and should help to advance the microbial lipid technology.

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更新日期/Last Update: 2017-12-25