|本期目录/Table of Contents|

[1]吴尧,万霞,张银波,等.白地霉(lip42)脂肪酶的纯化及酶学性质[J].应用与环境生物学报,2010,16(05):710-713.[doi:10.3724/SP.J.1145.2010.00710]
 WU Yao,WAN Xia,ZHANG Yingbo,et al.Purification and Properties of a Lipase from Geotrichum candidum lip42[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):710-713.[doi:10.3724/SP.J.1145.2010.00710]
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白地霉(lip42)脂肪酶的纯化及酶学性质()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年05期
页码:
710-713
栏目:
研究论文
出版日期:
2010-10-25

文章信息/Info

Title:
Purification and Properties of a Lipase from Geotrichum candidum lip42
文章编号:
200911018
作者:
吴尧万霞张银波黄凤洪陈洪江木兰
(1中国农业科学院油料作物研究所 武汉 430062)
(2农业部油料作物生物学重点实验室 武汉 430062)
Author(s):
WU YaoWAN XiaZHANG YingboHUANG FenghongCHEN HongJIANG Mulan
(1Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
(2Key Laboratory of Oil Crops Biology of Ministry of Agriculture, Wuhan 430062, China)
关键词:
白地霉lip42脂肪酶酶活性纯化酶学性质
Keywords:
Geotrichum candidum lip42 lipase enzyme activity purification enzyme properties
分类号:
Q939.9 : Q556
DOI:
10.3724/SP.J.1145.2010.00710
文献标志码:
A
摘要:
白地霉(Geotrichum candidum)经过发酵培养,以发酵液为lip42脂肪酶粗酶液,经硫酸铵分级沉淀、透析脱盐、DEAE纤维素柱层析及Sephadex-75凝胶柱层析纯化处理,脂肪酶比活力达到121.68 U/mg,纯化倍数为粗酶液的7.3倍,回收率为30.25%,经SDS-PAGE电泳显示为单一条带,相对分子质量约64×103. 脂肪酶的酶学性质研究结果表明:在40 ℃以下温度保存1 h内酶活性相对稳定;在pH 8.0和30 ℃反应条件下表现相对高的酶活性;在Mg2+存在情况下酶活性提高10%左右,而其它离子如Cu2+、Zn2+等以及0.1%的Tween 80、Tween 20及Triton-100对酶活性却有不同程度的抑制作用,1 mmol/L的SDS和EDTA-Na2对酶活性的影响很微小. 图7 表2 参15
Abstract:
The crude lipase from Geotrichum candidum lip42 fermentation broth was purified to homogeneity using ammonium sulfate fractional precipitation, dialysis, DEAE-Cellulose anion exchange chromatography and Sephadex G-75 gel filtration chromatography. These protocols resulted in a 7.3-fold purification with a high specific activity of 121.68 U/mg and 30.25% final yield of the enzyme. The relative molecular weight of the enzyme was determined to be approximately 64×103 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum pH and temperature for activity of the lipase were 8.0 and 30 ℃, respectively. The activity of the lipase was comparatively stable at 40 ℃ for a hour. The lipase activity was enhanced in the presence of Mg2+ by 10%, whereas it was inhibited by 0.1% Tween 80, Tween 20, Triton-100 and the other ions, such as Cu2+ and Zn2+. SDS and EDTA-Na2 showed little effect on the activity of the lipase at 1 mmol/L concentration. Fig 7, Tab 2, Ref 15

参考文献/References:

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备注/Memo

备注/Memo:
国家“863”计划现代农业技术领域重点项目、湖北省农业创新中心项目和武汉市青年晨光计划资助 Supported by the Key Project of the National “863” Program of China for Agriculture Technology, the Agriculture Innovation Project of Hubei, China and the Youth Project of Wuhan, China
更新日期/Last Update: 2010-10-25