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[1]贾志红,易建华,苏以荣,等.云南玉溪烟区轮作与连作土壤细菌群落多样性比较研究[J].应用与环境生物学报,2011,17(02):162-168.[doi:10.3724/SP.J.1145.2011.00162]
 JIA Zhihong,YI Jianhua,SU Yirong,et al.Comparison of Soil Bacterial Diversity in Rotation and Monocropping Soil in Tobacco Growing Area in Yuxi, Yunnan, China[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):162-168.[doi:10.3724/SP.J.1145.2011.00162]
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云南玉溪烟区轮作与连作土壤细菌群落多样性比较研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年02期
页码:
162-168
栏目:
研究论文
出版日期:
2011-04-25

文章信息/Info

Title:
Comparison of Soil Bacterial Diversity in Rotation and Monocropping Soil in Tobacco Growing Area in Yuxi, Yunnan, China
作者:
贾志红 易建华 苏以荣 曾军英
(1中国科学院亚热带农业生态研究所 长沙 410125)
(2中国科学院研究生院 北京 100049)
(3湖南中烟工业有限责任公司 长沙 410007)
(4湖南怀化学院 怀化 418000)
Author(s):
JIA Zhihong YI Jianhua SU Yirong ZENG Junying
(1Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
(3China Tobacco Hunan Industrial Co., Ltd, Changsha 410007, China)
(4Huaihua University, Huaihua 418000, Hunan, China)
关键词:
轮作连作PCR-DGGE土壤细菌群落多样性烤烟
Keywords:
rotation monocropping PCR-DGGE soil microbe diversity flue-cured tobacco
分类号:
S154.36 : S344
DOI:
10.3724/SP.J.1145.2011.00162
文献标志码:
A
摘要:
为评价云南玉溪烟区轮作和连作对土壤细菌群落多样性影响,从土壤中直接提取土壤细菌总DNA,用细菌16S rDNA特异性引物进行PCR扩增和变性梯度凝胶电泳(DGGE),每个处理样品3次重复,在DGGE图谱中相似性较高,基本聚在一起,从整体证明了试验操作方法较为精确. 从泳道分析图可知,轮作土壤细菌群落各泳道条带数量13~29条不等,平均为21 条. 连作土壤细菌群落各泳道条带数量从4~20 条不等,平均为12条. 从每条泳道的条带数方面和光密度值分别进行细菌群落多样性各指标的比较,结果表明,轮作处理细菌群落丰富度指数均大于连作处理,轮作香农-威纳和辛普森指数(1/D)均大于连作,表明轮作微生物多样性较连作高,轮作方式可以提高植烟土壤细菌群落的多样性. 图5 表2 参20
Abstract:
To evaluate the responses of soil bacteria community to two different tobacco cropping systems (rotation and monocropping), soil bacteria were examined by culture-independent means, namely, 16S rDNA PCR-DGGE profiling. Crude DNA was extracted from the rotation and monocropping soil, amplied with bacterial specific primers of 16S rDNA by PCR, and separated by denaturing gradient gel electrophoresis (DGGE). Subsequently, bands on DGGE were used to infer the diversity of bacteria in the rotation and monocropping soil. Three duplications of each treatment in the DGGE profile had higher similarity, almost getting together, showing the experiment premise for operation method. From the DGGE profile, the bacterial community bands in the rotation soil were found about 13~29, with average of 21, but the bands in the monocropping soil about 4~20, with average of 12. The comparison of the electrophoretic bands and optical density value with each index of bacterial richness and diversity showed the richness of the bacterial community in the rotation soil was higher than that in the monocropping soil. The Shannon-wiener index and Simpson index (1/D) of the rotation soil were all higher than those of the monocropping soil, which indicates that the rotation farming system can improve the bacterial diversity of soil. Fig 5, Tab 2, Ref 20

参考文献/References:

1 Rice EL. Allelopathy Second Edition. New York, USA: Academy Press Inc., 1984. 151~187
2 Lei JL (雷娟利), Zhou YH (周艳虹), Ding J (丁桔), Wang L (王礼), Yu JQ (喻景权). Effect of continous cropping of different vegetables on DNA polymorphorism of soil bacterial. Sci Agric Sin (中国农业科学), 2005, 38 (10): 2076~2083
3 Kennedy AC. Bacterial diversity in agroecosystems. Agric Ecosyst & Environ,1999, 74: 65~76
4 Chen XR (陈秀蓉), Nan ZB (南志标). Bacterial diversity and its role in agricultural ecosystems. Pratacult Sci (草业科学), 2002, 19 (9): 34~38
5 Smith KP, Goodman RM. Host variation for interactions with beneficial plant-associated microbes. Annu Rev Phytopathol, 1999, 37: 473~491
6 Broke TD. The study of microorganisms in situ: Progress and problems. Symp Soc Gene Microbiol, 1987, 41: 1~17
7 Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev, 1995, 59 (1): 143~169
8 Luo HF (罗海峰), Qi HY (齐鸿雁), Xu K (薛凯), Zhang HX (张洪勋). A preliminary application of PCR-DGGE to study microbial diversity in soil. Acta Ecol Sin (生态学报), 2003, 23 (8): 1570~1575
9 Hedrick DB, Peacock A, Stephen JR, Macnaughton SJ, Brüggemann J, White DC. Measuring soil microbial community diversity using polar lipid fatty acid and denaturing gradient gel electrophoresis data. J Microbiol Methods, 2000, 41: 235~248
10 Li ZG (李志岗), Yang GP (杨官品), Zhu YH (朱艳红). The relationship between polymorphism of bacterial 16S rDNA restriction fragment length and community structure of aquatic bacteria. Acta Hydrobiol Sin (水生生物学报), 2001, 25 (2): 111~114
11 Muyzer G. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol, 1999, 2: 317~322
12 Sigler WV, Turco RF. The impact of chlorothaloml application on soil bacterial and fungal populations as assessed by denaturing gradient gel electrophoresis. Appl Soil Ecol, 2002, 21: 107~118
13 Muyzer G, Smalla K. Application of denaturing gradient gel electroporesis (DGGE) and temperature gradient gel electroporesis (TGGE) in microbial ecology. Antoni Van Lereuwenhoek, 1998, 73: 127~141
14 Zhao JH (赵继红), Lou Y (楼燕), Yu ZC (余志晟). The microbial community structure and dynamic changes in municipal wastewater treatment plants (A/O). Ecol & Environ (生态环境), 2008, 17 (3): 898~902
15 Gao YB (高亦豹), Wang HY (王海燕), Xu Y (徐岩). PCR-DGGE analysis of the bacterial community of Chinese liquor high and medium temperature Daqu. Microbiol China (微生物学通报), 2010, 37 (7): 999~1004
16 Chen DM (陈冬梅), Ke WH (柯文辉), Chen LL (陈兰兰), Huang JW (黄锦文), Wu WX (吴文祥), Chen T (陈婷), Zhang ZY (张重义), Lin WX (林文雄). Diversity of bacterial community in rhizosphere soils under effects of continuously planting burley tobacco. Chin J Appl Ecol (应用生态学报), 2010, 21 (7): 1751~1758
17 Hu YS (胡元森), Wu K (吴坤), Li CX (李翠香), Jia XC (贾新成). Effect of continuous cropping of cucumber on soil microbial population Ⅱ—Variation analysis based on DGGE approach. Sci Agric Sin (中国农业科学), 2007, 40 (10): 2267~2273
18 Zhou J, Bruns MA, Tiedje JM. DNA recovery from soils of diverse composition. Appl & Environ Microbiol, 1996, 62: 316~322
19 Zhong WH (钟文辉), Cai ZC (蔡祖聪), Yin LC (尹力初), Zhang H (张鹤). The effects of the long-term application of inorganic fertilizers on microbial community diversity in rice-planting red soil as studied by PCR-DGGE. Acta Ecol Sin (生态学报), 2007, 27 (10): 4011~4018
20 Andreote FD, Mendes R, Dini-Andreote F, Rossetto PB, Labate CA, Pizzirani-Kleiner AA, Van Elsas JD, Azevedo JL, Araújo WL.Transgenic tobacco revealing altered bacterial diversity in the rhizosphere during early plant development. Antonie Van Leeuwenhoek, 2008, 93 (4): 415~424

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备注/Memo

备注/Memo:
中国科学院知识创新工程重要方向项目(No. KSCX2-YW-N-48-1)和湖南中烟工业有限责任公司科研项目(No. 2007-YC-001)资助
更新日期/Last Update: 2011-04-25