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[1]刘招龙,张绍铃,高富永.外源水杨酸对梨叶片感染轮纹病菌后抗氧化酶活性及Ca2+浓度的影响[J].应用与环境生物学报,2011,17(02):215-218.[doi:10.3724/SP.J.1145.2011.00215]
 LIU Zhaolong,ZHANG Shaoling,GAO Fuyong.Effect of Exogenous Salicylic Acid on Antioxidant Enzymes and Ca2+ Density in Pear Leaves Infected by Physalosproa piricola Nose[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):215-218.[doi:10.3724/SP.J.1145.2011.00215]
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外源水杨酸对梨叶片感染轮纹病菌后抗氧化酶活性及Ca2+浓度的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年02期
页码:
215-218
栏目:
研究论文
出版日期:
2011-04-25

文章信息/Info

Title:
Effect of Exogenous Salicylic Acid on Antioxidant Enzymes and Ca2+ Density in Pear Leaves Infected by Physalosproa piricola Nose
作者:
刘招龙 张绍铃 高富永
(1南京农业大学园艺学院 南京 210095)
(2宁德师范学院 宁德 352100)
Author(s):
LIU Zhaolong ZHANG Shaoling GAO Fuyong
(1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
(2Ningde Normal University, Ningde 352100, Fujian, China)
关键词:
砂梨轮纹病菌水杨酸抗氧化酶Ca2+.
Keywords:
Pyrus serotina Rehd Physalospore piricola Nose salicylic acid antioxidant enzyme Ca2+
分类号:
S432.26 : S436.612.1
DOI:
10.3724/SP.J.1145.2011.00215
文献标志码:
A
摘要:
为了解水杨酸(Salicylic acid,SA)缓解轮纹病菌伤害植物的机理,用0.3 mmol L-1的SA预先处理梨叶片,3 d后接种轮纹病菌(Physalospora Piricola Nose),接种4 d后开始测定各种指标,随后每隔3 d测定1 次,共5 次. 结果表明,经0.3 mmol L-1 SA处理后,梨叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)和谷胱甘肽还原酶(GR)的活性分别比对照组(CK1)提高了30.65%、25.36%、28.55%、21.11%、35.67%和26.45%. 电镜观察表明,感染轮纹病菌的梨叶片,Ca2+较多分布在细胞膜内侧、细胞质及一些细胞器(叶绿体)上. 经SA处理后感染轮纹病菌的梨病叶,Ca2+较多分布在细胞壁外侧及细胞间隙中,与正常梨叶片Ca2+的分布情况相似. 表明较低浓度的SA可缓解轮纹病菌对梨叶片的伤害作用. 图3 参14
Abstract:
Salicylic acid, a plant induced-resistance activator, is able to alleviate the injury of pathogen to plant. In this study, 0.3 mmol L-1 salicylic acid (SA) was applied on the leaves of pear, after 3 d Physalosproa piricola Nose was inoculated, and 4 d after inoculation all indexes were measured. The measurement was made every 3 days for 5 times. The results showed that compared with CK1, the 0.3 mmol L-1 SA could increase the activities of SOD, POD, CAT, APX, GPX and GR proline in leaves by 30.65%, 25.36%, 28.55%, 21.11%, 35.67% and 26.45%, respectively. The observation with TEM showed that in the pear leaves infected by P. piricola, Ca2+ was distributed on the inside of cell film, and on cytoplasm and some cell organs (such as chloroplast). The SA treatment indicated that inside the vascular bundle, secretion of germina could be seen, Ca2+ was mostly dotted on the outside of cell wall and between cells, which was similar to that in normal pear leaves, indicating that lower concentration of SA could reduce the damage of pear leaves by P. piricola. Fig 3, Ref 14

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相似文献/References:

[1]刘招龙,张绍铃,孙益林.水杨酸对梨叶感染轮纹菌后脂肪酸、叶绿素荧光参数和叶绿体超微结构的影响[J].应用与环境生物学报,2008,14(01):43.
 LIU Zhaolong,et al..Effect of Salicylic Acid on Fatty Acid, Chlorophyll Fluorescence Parameters and Chloroplast Ultrastructure of Pear Leaves Infected by Physalosproa piricola Nose[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):43.

备注/Memo

备注/Memo:
国家自然科学基金项目(No. 30170651)资助
更新日期/Last Update: 2011-04-25