|本期目录/Table of Contents|

[1]王丽平,郑丙辉.天津新港区潮间带沉积物细菌群落结构[J].应用与环境生物学报,2011,17(03):303-306.[doi:10.3724/SP.J.1145.2011.00303]
 WANG Liping,ZHENG Binghui.Bacterial Community Structure in Intertidal Sediment in Xingang Area, Tianjin, China[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):303-306.[doi:10.3724/SP.J.1145.2011.00303]
点击复制

天津新港区潮间带沉积物细菌群落结构()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年03期
页码:
303-306
栏目:
研究论文
出版日期:
2011-06-24

文章信息/Info

Title:
Bacterial Community Structure in Intertidal Sediment in Xingang Area, Tianjin, China
作者:
王丽平郑丙辉
(中国环境科学研究院国家环保部河流与海岸带重点实验室 北京 100012)
Author(s):
WANG LipingZHENG Binghui
(Estuary & Coastal Key Laboratory of State Environmental Protection, Chinese Research Academy of Environmental Sciences, Beijing 100012, China)
关键词:
天津新港潮间带沉积物细菌群落多样性PCR-DGGE变形菌门
Keywords:
Xingang area Tianjin intertidal sediment bacterial community diversity PCR-DGGE Proteobacteria
分类号:
Q939.181.5 : X55
DOI:
10.3724/SP.J.1145.2011.00303
文献标志码:
A
摘要:
利用PCR-DGGE技术对天津新港潮间带(东经117°45′11.4″~45′12.4″,北纬 39°2′42.8″~3′14.6″)沉积物中的细菌群落组成和优势菌群进行了调查,通过Quantity One软件分析不同样品的DGGE图谱,发现9个站位的细菌种类、数量和多样性都存在明显差别,表明该潮间带细菌群落的空间异质性较高. 细菌16S rDNA V3区特征片段经DGGE分离、条带切割,克隆、测序后,进行BLAST比对和系统进化分析,结果表明天津新港潮间带沉积物中主要优势菌群归属于未培养的变形菌门(Proteobacteria)和其它一些环境样品中的未归类菌群的细菌克隆;同时还发现许多条带序列与来自受不同污染的环境样品中的未培养细菌克隆具有较高的同源性(94%~100%),提示该区域受到不同类型污染物(如硫化物、重金属以及芳烃类污染物)的污染. 图2 表3 参12
Abstract:
The bacterial community structure and predominant bacterial species were investigated by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) in intertidal sediment in Xingang Area (E 117°45′11.4″~45′12.4″, N 39°2′42.8″~3′14.6″), Tianjin, China. The DGGE profile was analyzed by Quantity One Software, and the results showed that there were obvious differences in bacterial species, number and population diversity among the 9 sediment samples, which indicated high spatial heterogeneity of bacterial community in this area. Twenty DGGE bands reflecting predominant phylotypes were excised, cloned, sequenced and BLAST. The results showed that the bacterial phylotypes were composed of uncultured Proteobacteria and other unclassified bacterial clones from environmental samples. 16S rDNA sequences of some bands had high homology (94%~100%) with the uncultured bacterial clones isolated from different polluted environment samples, which suggested the studied area polluted by various contaminants (e.g. sulfide, heavy metal, aromatic hydrocarbon). Fig 2, Tab 3, Ref 12

参考文献/References:

1 Lasse R, Grieg FS, Laura BF. Bacterial community composition during two consecutive NE Monsoon periods in the Arabian Sea studied by denaturing gradient gel electrophoresis (DGGE) of rRNA genes. Deep-Sea Res II, 1999, 46 (6): 1791~1811
2 Amann RI, Ludwing W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev, 1995, 59: 143~169
3 Pace NR. A molecular view of microbial diversity and the biosphere. Science, 1997, 276: 734~740
4 Muyzer G, Dewaal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol, 1993, 59: 695~700
5 Feng S (冯胜), Gao G (高光), Zhu GW (朱广伟), Zhang YL (张运林), Qin BQ (秦伯强). Bacterial community structure in different eco-restoration areas in a eutrophic lake based on 16S rDNA DGGE and FDC. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (4): 535~540
6 Ma JX (马俊孝), Kong J (孔健), Ji MJ (季明杰). Detection of the lactic acid bacteria in commercial yoghurts by PCR-denaturing gradient gel electrophoresis. Chin J Appl Environ Biol (应用与环境生物学报), 2009, 15 (4): 534~539
7 Liu GH, Amemiya T, Itoh K. Two-dimensional DNA gel electrophoresis mapping: a novel approach to diversity analysis of bacterial communities in environmental soil. J Biosci Bioeng, 2008, 105 (2): 127~133
8 Pennanen T, Paavolainen L, Hantula J. Rapid PCR-based method for the direct analysis of fungal communities in complex environmental samples. Soil Biol Biochem, 2001, 33: 697~699
9 Ercolini D, Mauriello G, Blaiotta G, Moschetti G, Coppola S. PCR-DGGE fingerprints of microbial succession during a manufacture of traditional water buffalo mozzarella cheese. J Appl Microbiol, 2004, 96: 263~270
10 Zak JC, Willig MR, Moorhead DL, Wildman HG. Functional diversity of microbial communities - a quantitative approach. Soil Biol Biochem, 1994, 26: 1101~1108
11 Gao PP (高平平), Zhao LP (赵立平). Capacity evaluation of different media for isolating predominant phenol-degrading bacteria from activated sludge with community structure-specific DNA probes. Acta Microbiol Sin (微生物学报), 2003, 43 (2): 264~270
12 Kanagawa T. Bias and artifacts in multitemplate polymerase chain reaction (PCR). J Biosci Bioeng, 2003, 96: 317~323

备注/Memo

备注/Memo:
国家基础研究发展规划项目(“973”项目)(No. 2007CB407306)资助
更新日期/Last Update: 2011-06-23