|本期目录/Table of Contents|

[1]刘洋,栗敏,郭泽经,等.一株中度嗜盐淀粉酶产生菌的分离鉴定及酶活测定[J].应用与环境生物学报,2011,17(06):883-886.[doi:10.3724/SP.J.1145.2011.00883]
 LIU Yang,LI Min,GUO Zejing,et al.Isolation and Identification of a Moderately Halophilic Amylase-producing Strain and Determination of Amylase Activity[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):883-886.[doi:10.3724/SP.J.1145.2011.00883]
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一株中度嗜盐淀粉酶产生菌的分离鉴定及酶活测定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年06期
页码:
883-886
栏目:
研究论文
出版日期:
2011-12-25

文章信息/Info

Title:
Isolation and Identification of a Moderately Halophilic Amylase-producing Strain and Determination of Amylase Activity
作者:
刘洋栗敏郭泽经何艳冯治翔宋昭彬田永强
(1四川大学生命科学学院 成都 610065)
(2四川大学化学工程学院 成都 610065)
(3中国医药集团四川抗菌素工业研究所 成都 610065)
Author(s):
LIU YangLI MinGUO ZejingHE YanFENG ZhixiangSONG ZhaobinTIAN Yongqiang
(1College of Life Sciences, Sichuan University, Chengdu 610065, China)
(2College of Chemical Engineering, Sichuan University, Chengdu 610065, China)
(3Sichuan Industrial Institute of Antibiotics, China National Pharmaceutical Group Corporation, Chengdu 610065, China)
关键词:
嗜盐细菌菌株鉴定Gracilibacillus sp.系统进化淀粉酶酶活测定高盐碱环境
Keywords:
halophilic bacterium strain identification Gracilibacillus sp. phylogenetic analysis amylase enzyme activity determination high salt and alkaline condition
分类号:
Q936
DOI:
10.3724/SP.J.1145.2011.00883
文献标志码:
A
摘要:
为开发极端环境工业用酶,从新疆盐碱土微生物中分离得到一株中度嗜盐高产淀粉酶活性菌株H3,其能耐受30%盐浓度和pH 11的极端环境. 通过形态特征、生理生化实验及16S rRNA基因序列分析,确定H3属于Gracilibacillus属. 该菌株能在盐浓度为0~30%的培养基上生长,最适生长盐浓度为5%~10%,最适pH值为8.5. 在最适生长盐浓度、pH条件下,其淀粉酶活性可达到4 830个活力单位,可用于高盐高碱环境下淀粉的水解. 图4 表2 参18
Abstract:
A high-yield amylase strain, named as H3, was isolated from saline-alkali soil in Xinjiang, China. H3 could grow under the condition with 30% NaCl concentration and pH 11. Based on the results of morphological, physiological and biochemical experiments, and analysis of 16S rRNA sequence, this strain was identified as Gracilibacillus sp. It could grow on the media containing 0~30% NaCl. The optimum NaCl concentration for H3 growth was 5%~10% and the optimum pH was 8.5. The highest amylase activity was 4 830 units under the optimum condition for H3 growth, which suggesting that this strain could be used for starch hydrolysis under high salt and alkaline condition. Fig 4, Tab 2, Ref 18

参考文献/References:

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14 Kobayashi T, Kanai H, Hayashi T, Akiba T, Akaboshi R, Horikoshi K. Haloalkaliphilic maltotriose-forming α-amylase from the archaebacterium Natronococcus sp. strain Ah-36. J Bacteriol, 1992, 174 (11): 3439~3444
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更新日期/Last Update: 2011-12-31