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[1]丁栋,王博禅,陈桂琛,等.一株抗肿瘤放线菌的鉴定及生物活性[J].应用与环境生物学报,2012,18(06):983-987.[doi:10.3724/SP.J.1145.2012.00983]
 DING Dong,WANG Bochan,CHEN Guichen,et al.Identification and Bioactivity of an Antitumor Actinomycete[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):983-987.[doi:10.3724/SP.J.1145.2012.00983]
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一株抗肿瘤放线菌的鉴定及生物活性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年06期
页码:
983-987
栏目:
研究论文
出版日期:
2012-12-25

文章信息/Info

Title:
Identification and Bioactivity of an Antitumor Actinomycete
作者:
丁栋 王博禅 陈桂琛 王启兰 彭敏 史萍
(1中国科学院西北高原生物研究所 西宁 810001)
(2中国科学院研究生院 北京 100049)
(3华东理工大学生物反应器工程国家实验室 上海 200237)
Author(s):
DING Dong WANG Bochan CHEN Guichen WANG Qilan PENG Min SHI Ping
(1Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
(3State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China)
关键词:
青藏高原青海湖沙岛放线菌砖红链霉菌抗肿瘤菌株鉴定生物活性
Keywords:
Qinghai-Tibet plateau Qinghai lake desert island actinomycete Streptomyces lateritius antitumor strain identification bioactivity
分类号:
Q939.130.5 (244)
DOI:
10.3724/SP.J.1145.2012.00983
文献标志码:
A
摘要:
为开发青藏高原沙地生态系统放线菌资源,首次从位于青藏高原东北部的青海湖沙岛土壤样品中选择性分离培养出一株具明显抑菌及抗肿瘤活性放线菌Sd-31. 通过生理生化特性、化学分类学以及16S rRNA序列分析鉴定,菌株为砖红链霉菌,命名为Streptomyces lateritius Sd-31;双层琼脂法检测菌株抑菌活性,表明菌株对金黄色葡萄球菌(Staphyloccocus aureus)以及枯草芽孢杆菌(Bacillus subtilis)有显著抑制作用;通过Hoechst染色荧光显微镜观察及MTT法细胞毒性试验,确定菌株发酵液有明显的诱导肿瘤细胞凋亡活性且与处理浓度成正相关;纸片法研究菌株发酵液活性稳定性,结果表明,20~80 ℃处理后发酵液抑菌活性稳定,100 ℃条件处理后发酵液抑菌活性降低;在酸性及中性条件下发酵液活性稳定,碱性条件下随着pH值的增加发酵液抑菌活性降低. 以上结果为抗肿瘤药物的研究提供了理论数据. 图6 表4 参23
Abstract:
To exploit actinomycetes from desert ecosystem of Qinghai-Tibet plateau, an antimicrobial and antitumor actinomycete Sd-31 was selectively isolated for the first time from the soil sample of the desert island in the Qinghai Lake, the northeast of Qinghai-Tibet plateau. According to physio-biochemical, chemotaxonomy and 16S rRNA gene sequence analysis, the strain was identified and named Streptomyces lateritius Sd-31. The antimicrobial bioactivity of the strain was tested by double layer agar method, and the result showed that the strain could inhibit the growth of Staphyloccocus aureus and Bacillus subtilis. According to Hoechst fluorescence microscope observation and cytotoxic test by MTT, the broth of the strain showed obvious bioactivity of leading to the apotosis of tumor cell. The stability of the broth bioactivity was tested by paper disk method, and the result showed that the antimicrobial bioactivity was stable between 20 ℃ and 80 ℃ and decreased at 100 ℃. The antimicrobial bioactivity was stable at acid and neutral treatment and decreasing with the increasing of pH at alkaline treatment. These findings provided data for exploring antitumor drugs. Fig 6, Tab 4, Ref 23

参考文献/References:

1 Zhao K, Penttinen P, Guan T, Xiao J, Chen Q, Xu J, LindstrÖm K, Zhang LL, Zhang XP, Strobel GA. The diversity and anti-microbial activity of endophytic actinomycetes isolated from medicinal plants in Panxi plateau, China. Curr Microbiol, 2011, 62: 182~190
2 Okoro CK, Brown R, Jones AL, Andrews BA, Asenjo JA, Goodfellow M, Bull AT. Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Deserts, Chile. Antonie Van Leeuwenhoek, 2009, 95: 121~133
3 Jiang SM, Sun W, Chen MJ, Dai SK, Zhang L, Liu YH, Lee KJ, Li X. Diversity of culturable actinobacteria isolated from maring sponge Haliclona sp. Antonie Van Leeuwenhoek, 2007, 92 (4): 405~416
4 Zenova G.M, Kurapova AI, Lysenko AM, Zvyagintsev DG. The structural-functional organization of the thermotolerant complexes of actinomycetes in desert and volcanic soils. Eurasian Soil Sci, 2009, 42 (5): 531~535
5 Si MR (司美茹), Xue QH (薛泉宏), Chen ZQ (陈占全), Yu B (余博), Cai Y (蔡艳). Ecological distribution of soil antimicrobial actinomycetes of the Qinghai plateau. Chin J Appl Environ Biol (应用与环境生物学报), 2005, 11 (1): 104~111
6 Tohty D (迪丽拜尔? 托乎提), Habdin X (旭格拉? 哈布丁), Chen J (陈瑾), Zhu YL (朱艳蕾), Ha SJ (艾山江), An DD (安登第), Zhou YG (周宇光). Biological characteristics and active substances of alkalophilic actinomycetes from extreme environment of Lop Nur Peripherals in Xinjiang, China. Chin J Appl Environ Biol (应用与环境生物学报), 2009, 15 (6): 835~839
7 Fan LX (范丽霞), Zheng JP (郑继平), Yang XH (杨先会). The effect of natural air-dry time on actinomycetes isolation from sample soil. J Hainan Med Coll (海南医学院学报), 2010, 16 (3): 280~284
8 Jiang Y (姜怡), Duan SR (段淑蓉), Tang SK (唐蜀昆), Chen HH (陈华红), Li WJ (李文均), Xu LH (徐丽华). Some approaches for the selective isolation of rare actinomycetes. Microbiology (微生物学通报), 2006, 33 (1): 181~183
9 Jiang Y (姜怡), Cao YR (曹艳茹), Zhao LX (赵立兴), Wang Q (王茜), Le RX (勒荣线), He WX (和文祥), Xue QH (薛泉宏). Ultrasonic treatment of soil samples for actinomycete isolation. Acta Microbiol Sin (微生物学报), 2010, 50 (8): 1094~1097
10 Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol, 1974, 28 (2): 226~231
11 Ruan JS (阮继生), Huang Y (黄英). Rapid Identification and Systematics of Actinobacteria. Beijing, China: Science Press (北京: 科学出版社), 2011. 69~116
12 Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol, 1974, 28 (2): 226~231
13 Xu LH (徐丽华), Li WJ (李文均), Liu ZH (刘志恒), Jiang CL (姜成林). Actinomycete Systematic—Principle, Methods and Practice. Beijing, China: Science Press (北京: 科学出版社), 2007. 80~87
14 Heuer H, Krsek M, Baker P, Smalla K, Wellington EMH. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl Environ Microbiol, 1997, 63: 3233~3241
15 Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol Biol Evol, 2007, 24 (8): 1596~1599
16 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876~4882
17 Chen J, Chen HM, Zhang YQ, Wei YZ, Li QP, Liu HY, Su J, Zhang YQ, Yu LY. Agromyces flavus sp. nov., an actinomycete isolated from soil. Int J Syst Evol Microbiol, 2011, 61 (7): 1705~1709
18 Zhang YQ, Liu HY, Chen J, Yuan LJ, Sun W, Zhang LX, Zhang YQ, Yu LY, Li WJ. Diversity of culturable actinobacteria from Qinghai-Tibet Plateau, China. Antonie Van Leeuwenhoek , 2010, 98: 213~223
19 Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CL. Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol, 2005, 55: 1867~1870
20 Zhang YQ, Schumann P, Yu LY, Liu HY, Zhang YQ, Xu LH, Stackebrandt E, Jiang CL, Li WJ. Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae. Int J Syst Evol Microbiol, 2007, 57: 1018~1023
21 Tuntiwachwuttikul P, Taechowisan T, Wanbanjob A, Thadaniti S, Taylor WC. Lansai AD, secondary metabolites from Streptomyces sp. SUC1. Tetrahedron, 2008, 54: 7583~7586
22 Gilpin ML, Box SJ, Elson AL. New quinine antibiotics of the granaticin type, isolated from Streptomyces Lateritius Ⅱ. Structure determination. J Antibiotics, 1988, XLI (4): 512~518
23 Fleck WF, Stranss DG, Prauser H. Naphthoquinone antibiotics from Streptomyces lateritius. I fermentation, isolation and characterization of granatomycins A, C and D. Z Allg Mikrobiol, 1980, 20 (9): 543~551

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备注/Memo

备注/Memo:
国家科技支撑项目(No. 2007BAC30B04)资助 Supported by the Sci-tech Project of China (No. 2007BAC30B04)
更新日期/Last Update: 2012-12-28