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[1]刘悦萍,王子健,崔凯,等.一种野生香蘑的分离、鉴定、培养条件与产酶特性[J].应用与环境生物学报,2012,18(05):804-809.[doi:10.3724/SP.J.1145.2012.00804]
 LIU Yueping,WANG Zijian,CUI Kai,et al.Isolation, Identification, Culture Conditions, and Enzyme Production of a Wild Mushroom Lepista sp.[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):804-809.[doi:10.3724/SP.J.1145.2012.00804]
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一种野生香蘑的分离、鉴定、培养条件与产酶特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年05期
页码:
804-809
栏目:
研究论文
出版日期:
2012-10-25

文章信息/Info

Title:
Isolation, Identification, Culture Conditions, and Enzyme Production of a Wild Mushroom Lepista sp.
作者:
刘悦萍王子健崔凯张国庆
(北京农学院农业部都市农业(北方)重点实验室 北京 102206)
Author(s):
LIU Yueping WANG Zijian CUI Kai ZHANG Guoqing
(Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China)
关键词:
紫丁香蘑ITS鉴定培养条件漆酶Cu2+
Keywords:
Lepista nuda ITS identification culture condition laccase Cu2+
分类号:
Q949.320.5 : TQ925
DOI:
10.3724/SP.J.1145.2012.00804
文献标志码:
A
摘要:
为充分开发利用野生食用菌资源,从采集自北京怀柔的野生香蘑子实体中分离获得纯培养,编号为Lepista sp. GSM-11,结合形态学特征与ITS序列分析鉴定其分类学地位,并进一步研究其菌丝生长的最适碳源、氮源、C/N比、生长因子、最适温度和最适pH值,并测定了菌体液体发酵产漆酶、蛋白酶、植酸酶、核糖核酸酶和凝集素的特性,同时还研究了Cu2+对漆酶产量的影响. 结果表明,该菌株与GenBank中报道的采集自中国辽宁、美国马里兰州和日本宫城的紫丁香蘑(Lepista nuda)同源性最高,分别为100%、99.98%和99.93%,确定该野生香蘑为紫丁香蘑(ITS序列GenBank登录号JQ519375);菌丝体生长的最适碳源为蔗糖,最适氮源为酵母浸膏,最适C/N比为20/1,最适生长因子为维生素C,最适温度为24 ℃,最适pH为7.0;GSM-11菌株菌丝体和发酵液中均具有漆酶活性,但缺乏其他3种酶和凝集素活性. 1.0~2.0 mmol/L的Cu2+对漆酶分泌有显著的促进作用,其活性达到对照组的149.2%~196.4%. 图2 表6 参26
Abstract:
For efficient application of wild mushrooms, a strain of GSM-11 was isolated from a wild mushroom fruiting body of the genus Lepista, from Huairou, Beijing, and identified based on its morphology and ITS sequence analysis. The optimum culture conditions, i.e. carbon source, nitrogen source, C/N rate, growth factor, temperature, and pH value were investigated. And the enzyme activities of laccase, protease, phytase, ribonuclease, and lectin in liquid fermentation were also determined. The results showed that the strain (GenBank accession number of ITS sequence: JQ519375) manifested very high sequence similarity with L. nuda samples from Liaoning (China), Miyagi (Japan), and Maryland (USA) based on polymeric analysis of ITS sequences, with similarity of 100%, 99.98% and 99.93%, respectively. It was therefore identified as L. nuda. Culture conditions showed that the best carbon source was sucrose, the best nitrogen source was yeast extract, the best C/N rate was 20/1, the best growth factor was Vitamin C, the optimum temperature was 24 ℃, and the optimum pH was 7.0. Liquid fermentation studies showed that L. nuda demonstrated laccase activity in both mycelia and broth, but lack of the other four activities. A range of concentration of Cu2+ 1.0~2.0 mmol/L enhanced the laccase activity with a range of 149.2%~196.4% comparing with the control group. Fig 2, Tab 6, Ref 26

参考文献/References:

Willey JM, Sherwood LM, Woolverton CJ. Microorganisms in terrestrial evironments. In: McGraw-Hill ed. Prescott, Harley, and Klein’s Microbiology. 7th ed. Singapore, 2008. 687~712
Johnson NC, Wilson GW, Bowker MA, Wilson JA, Miller RM. Resource limitation is a driver of local adaptation in mycorrhizal symbioses. Proc Natl Acad Sci USA, 2010, 107 (5): 2093~2098
Wang B, Qiu YL. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza, 2006, 16 (5): 299~363
Rinaldi AC, Comandini O, Kuyper TW. Ectomycorrhizal fungal diversity: separating the wheat from the chaff. Fungal Div, 2008, 33: 1~45
Ng TB. Peptides and proteins from fungi. Peptides, 2004, 25 (6): 1055~1073
Mao XL (茆晓岚). The Macrofungi in China. Zhengzhou: Henan Science and Technology Press (郑州: 河南科技出版社), 2000. 178~179
Carluccio A. The Complete Mushroom Book. London: Quadrille Publishing Ltd, 2003. 155-156
Dulger B, Ergul CC, Gucin F. Antimicrobial activity of the macrofungus Lepista nuda. Fitoterapia, 2002, 73 (7~8): 695~697
Wu YY, Wang HX, Ng TB. A novel metalloprotease from the wild basidiomycete mushroom Lepista nuda. J Microbiol Biotechnol, 2011, 21 (3): 256~262
Gao JM (高锦明), Dong ZJ (董泽军), Yang X (杨雪), Liu, JK (刘吉开). Chemical constituents of Lepista nuda. Chin Trad & Herbal Drugs (中草药), 2002 (5): 17~20
Zhou F (周峰), Wang RJ (王瑞娟), Li Y (李玉), Yu HL (于海龙), Guo Q (郭倩). Biology and cultivation of the edible/medicinal fungus, Lepista nuda - a review. Acta Edulis Fungi (食用菌学报), 2010 (4): 79~83
Zhao T (赵婷), Chen YQ (陈艳秋), Li Y (李玉), Li CY (李宗云). Effect of cultural conditions on mycelial growth of Lepista nuda. Edible Fungi (食用菌), 2010 (3): 8~9
Wang HX (王贺祥). 食用菌学. Beijing,: China Agricultural University Press (北京: 中国农业大学出版社), 2004. 225~228
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11~15
Wang P, Liu Y, Yin Y, Jin H, Wang S, Xu F, Zhao S, Geng X. Diversity of microorganisms isolated from the soil sample surround Chroogomphus rutilus in the Beijing region. Int J Biol Sci, 2011, 7 (2): 209~220
Wang HX, Ng TB. A laccase from the medicinal mushroom Ganoderma lucidum. Appl Microbiol Biotechnol, 2006, 72 (3): 508~513
Cui L, Liu QH, Wang HX, Ng TB. An alkaline protease from fresh fruiting bodies of the edible mushroom Pleurotus citrinopileatus. Appl Microbiol Biotechnol, 2007, 75 (1): 81~85
Zhang GQ, Dong XF, Wang ZH, Zhang Q, Wang HX, Tong JM. Purification, characterization, and cloning of a novel phytase with low pH optimum and strong proteolysis resistance from Aspergillus ficuum NTG-23. Bioresour Technol, 2010, 101 (11): 4125~4131
Zhao JK, Wang HX, Ng TB. Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella. Toxicon, 2009, 53 (3): 360~366
Xie FG (谢富刚), Liu YY (刘雁英), Qiu HL (邱慧兰). Primary report of biological characteristics of Lepista nuda (Bull. Fr.) Cooke. Mod Agric Sci & Technol (现代农业科技), 2010 (2): 109~110
Zhao T (赵婷), Chen YQ (陈艳秋), Li Y (李玉). Characters of mycelial growth of Lepista nuda. Edible Fungi (食用菌), 2009 (6): 10~11
Zhang GQ, Wang YF, Zhang XQ, Ng TB, Wang HX. Purification and characterization of a novel laccase from the edible mushroom Clitocybe maxima. Process Biochem, 2010, 45 (5): 627~633
Dulger B, Ergul CC, Gucin F. Antimicrobial activity of the macrofungus Lepista nuda. Fitoterapia, 2002, 73 (7~8): 695~697
Wang HX, Ng TB, Ooi VE. Lectin activity in fruiting bodies of the edible mushroom Tricholoma mongolicum. Biochem Mol Biol Int, 1998, 44 (1): 135~141
Wang HX, Liu WK, Ng TB, Ooi VE, Chang ST. The immunomodulatory and antitumor activities of lectins from the mushroom Tricholoma mongolicum. Immunopharmacology, 1996, 31 (2~3): 205~211
Collins PJ, Dobson A. Regulation of Laccase Gene Transcription in Trametes versicolor. Appl Environ Microbiol, 1997, 63 (9): 3444~3450

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备注/Memo

备注/Memo:
“十二五”国家科技支撑计划课题(No. 2012BAD14B09)和北京市本科生科学研究计划项目资助
更新日期/Last Update: 2012-10-26