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[1]熊亚,陈强,唐玉明,等.泸州老窖不同窖龄窖泥中乳酸菌多样性PCR-DGGE分析[J].应用与环境生物学报,2013,19(06):1020-1024.[doi:10.3724/SP.J.1145.2013.01020]
 XIONG Ya,CHEN Qiang,TANG Yuming,et al.PCR-DGGE Analysis of Lactobacillus Diversity in Pit Muds of Different Cellar-aged Luzhou Laojiao Liquor[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):1020-1024.[doi:10.3724/SP.J.1145.2013.01020]
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泸州老窖不同窖龄窖泥中乳酸菌多样性PCR-DGGE分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年06期
页码:
1020-1024
栏目:
研究论文
出版日期:
2013-12-25

文章信息/Info

Title:
PCR-DGGE Analysis of Lactobacillus Diversity in Pit Muds of Different Cellar-aged Luzhou Laojiao Liquor
作者:
熊亚陈强唐玉明吴英英任道群陈翠平
(1四川农业大学资源与环境学院 成都 611130) (2攀枝花学院生物与化学工程学院 攀枝花 617000) (3四川省农业科学院水稻高粱研究所生物中心 泸州 646000)
Author(s):
XIONG Ya CHEN Qiang TANG Yuming WU Yingying REN Daoqun & CHEN Cuiping
(1College of Resource and Environment, Sichuan Agricultural University, Chengdu 611130, China) (2Department of Chemical and Biological Engineering, Panzhihua University, Panzhihua 617000, China) (3Biotechnology Center, Rice & Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646000, China)
关键词:
窖泥PCR-DGGE泸型酒乳酸菌多样性
Keywords:
cellar pit mud PCR-DGGE Luzhou-flavor lactic acid bacteria diversity
分类号:
TS261.1
DOI:
10.3724/SP.J.1145.2013.01020
文献标志码:
A
摘要:
乳酸菌是窖泥中的重要功能菌群,参与了浓香型白酒风味形成,因而探明窖泥中乳酸菌的种群特征,对于提高白酒品质具有重要意义. 采用PCR-DGGE技术对泸州老窖不同窖龄窖泥乳酸菌种群进行了分析. 结果显示,根据DGGE图谱条带情况,不同窖泥样品中乳酸菌多样性差异明显;多样性指数分析结果表明,样品的Patrick丰富度指数R介于14-19,Shannon-Wiener指数H值介于2.634 5-2.888 9之间,Simpson指数C值介于0.055 7-0.071 9. 根据图谱选取16条DGGE条带进行序列分析,它们分属于Lactobacillus(乳杆菌属)、Pediococcus(片球菌属)、Weissella(魏斯氏菌属)、Enterococcus(肠球菌属)、Exiguobacterium(微小杆菌属)、Bacillus(杆菌属)、Rummeliibacillus属以及Cohnella属,其中Lactobacillus(乳杆菌属)为优势菌属,L. acetotolerans(耐酸乳杆菌)为窖泥中乳酸菌的优势种群.
Abstract:
Lactobacillus is an important functional bacterium in pit mud, participating in the formation of strong aromatic liquor. Therefore exploring lactic acid bacteria species in pit mud is of great significance for the improvement of liquor quality. In this study, seven cellar pit mud samples were collected from different-aged cellars in Luzhou Laojiao Liquor Co. Ltd. The DNA was isolated, and the diversity of lactic acid bacteria was analyzed by PCR-DGGE technique. The results of DGGE showed significant diversity of lactic acid bacteria in the pit muds. Analysis of the diversity index showed the Patrick richness index R between 14 and 19; Shannon-Wiener index H varied between 2.634 5 and 2.888 9; and Simpson index C was between 0.055 7 and 0.055 7. Furthermore, 16 representative DGGE bands were picked, rinsed, and then amplified by using primers LAC1/LAC2, the PCR fragments were cloned and sequenced. The results revealed that the lactic acid bacteria in different-aged cellar pit mud samples were very abundant, belonging to genus Lactobacillus, Pediococcus, Weissella, Enterococcus, Exiguobacterium, Bacillus, Rummeliibacillus, and Cohnella, respectively. The predominant lactic acid bacteria among all the pit muds were Lactobacillus acetotolerans.

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