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[1]张笑,赵纯钦,黄静,等.外源脱落酸、水杨酸对小麦种子萌发及生理特性的影响[J].应用与环境生物学报,2014,20(01):139-143.[doi:10.3724/SP.J.1145.2014.00139]
 ZHANG Xiao,ZHAO Chunqin,HUANG Jing,et al.Effect of exogenous abscisic acid and salicylic acid on germination and physiological characteristics of wheat seed[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):139-143.[doi:10.3724/SP.J.1145.2014.00139]
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外源脱落酸、水杨酸对小麦种子萌发及生理特性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年01期
页码:
139-143
栏目:
研究简报
出版日期:
2014-02-25

文章信息/Info

Title:
Effect of exogenous abscisic acid and salicylic acid on germination and physiological characteristics of wheat seed
作者:
张笑赵纯钦黄静徐登安张赤红陈静
中国科学院成都生物研究所 成都 610041
Author(s):
ZHANG Xiao ZHAO Chunqin HUANG Jing XU Deng’an ZHANG Chihong CHEN Jing
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
小麦穗发芽脱落酸水杨酸种子萌发
Keywords:
wheat pre-harvest sprouting abscisic acid salicylic acid seed germination
分类号:
S512.103
DOI:
10.3724/SP.J.1145.2014.00139
文献标志码:
A
摘要:
穗发芽严重降低籽粒产量、品质和种用价值,是小麦育种和生产急需解决的难题. 为利用发芽抑制剂的化控途径有效防治穗发芽,研究了不同浓度的外源激素脱落酸(ABA)、水杨酸(SA)对种子发芽的抑制作用及其生理生化响应. 结果表明:浓度大于70 mg L-1 ABA和大于4 mmol L-1 SA均不同程度地抑制种子发芽并表现剂量效应,赤霉素对二者有拮抗作用,70 mg L-1 ABA+8.0 mmol L-1 SA对小麦普遍具有强烈的发芽抑制效应;ABA阻抑种子发芽是一种延缓作用,受基因型影响较大;SA对不同基因型有强烈的休眠促进作用,并显著提高种子对ABA的敏感性(P<0.01);种子萌发过程中,两种激素均诱导籽粒α-淀粉酶和PPO活性下降,且SA处理组的酶活性降幅更大;ABA明显促进种子PAL活性,而SA处理组的PAL活性水平最低. 因此,ABA与SA的发芽抑制效应与作用方式不同,SA增强种子的ABA敏感性,二者组合产生了良好、稳定的发芽抑制效果.
Abstract:
Pre-harvest sprouting (PHS), which causes devastating damage to grain yield and end-use quality, is a serious problem for wheat breeding and production in the world. Exogenous abscisic acid (ABA) and salicylic acid (SA) were used to study the effect of applied hormones on wheat seed germination, and the physiological chemistry of germinated seeds after hormone treatment. The results showed that both ABA (> 70 mg L-1) and SA (> 4 mmol L-1) blocked wheat seed germination in a dosage dependent manner whereas gibberellin antagonized the inhibitory effects slightly. Co-application of 70 mg L-1 ABA and 8 mmol L-1 SA commonly caused intensive germination inhibition without genotype-dependent variation. ABA displayed stronger germination inhibition on ABA-sensitive wheat genotypes than on ABA-insensitive ones, probably caused by postponing germination process. Comparatively SA showed more intensive effect on germination inhibition, with no correlation between inhibitory effects and wheat genotypes. SA significantly inhibited the transfer of seed development from dormancy to germination, and had noticeably positive effect on plant ABA sensitivity for inhibition of seed germination (P < 0.01). During seed germination, activities of alpha-amylase and polyphenol oxidase (PPO) decreased significantly when treated by either ABA or SA, with lower activity levels in SA group. Compared with the control, ABA significantly promoted phenylalanine ammonialyase (PAL) activity whereas SA group showed the lowest level of PAL activity. In conclusion, ABA and SA have different effect on seed germination in terms of inhibition extent and underlying mechanisms.SA increases seed sensitivity to ABA, and the hormone combination causes stable and intensive germination inhibition.

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备注/Memo

备注/Memo:
中国科学院知识创新工程重要方向项目(KSCX2-EW-J-22)、中国科学院西藏院地合作项目(XBCD-2011-019)和四川省小麦育种攻关项目资助
更新日期/Last Update: 2014-03-06