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[1]许光素,崔志松,郑立,等.高分子量多环芳烃降解过程中菌种间的相互作用[J].应用与环境生物学报,2013,19(04):677-682.[doi:10.3724/SP.J.1145.2013.00677]
 XU Guangsu,CUI Zhisong,ZHENG Li,et al.Bacterial Interaction in the Biodegradation of High Molecular Weight Polycyclic Aromatic Hydrocarbons[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):677-682.[doi:10.3724/SP.J.1145.2013.00677]
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高分子量多环芳烃降解过程中菌种间的相互作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年04期
页码:
677-682
栏目:
研究论文
出版日期:
2013-08-25

文章信息/Info

Title:
Bacterial Interaction in the Biodegradation of High Molecular Weight Polycyclic Aromatic Hydrocarbons
作者:
许光素崔志松郑立臧家业杨佰娟宋一之侯伟
(1国家海洋局第一海洋研究所海洋生态研究中心 青岛 266061)
(2清华大学环境学院 北京 100084)
(3青岛科技大学化学与分子工程学院 青岛 266042)
Author(s):
XU Guangsu CUI Zhisong ZHENG Li ZANG Jiaye YANG Baijuan SONG Yizhi HOU Wei
(1Marine Ecology Research Center, the First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China)
(2School of Environment, Tshinghua University, Beijing 100084, China)
(3College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China)
关键词:
高分子量多环芳烃解环菌属海杆菌属生物修复协同效应
Keywords:
high molecular weight polycyclic aromatic hydrocarbons Cycloclasticus Marinobacter bioremediation synergic effect
分类号:
X172 : X55
DOI:
10.3724/SP.J.1145.2013.00677
文献标志码:
A
摘要:
为研究海洋微生物间降解高分子量多环芳烃(High molecular weight - polycyclic aromatic hydrocarbons,简称HMW-PAHs)过程中的相互作用,筛选具有协同效应的菌群,以1株可降解HMW-PAHs的解环菌属细菌Cycloclasticus sp. PY97M和4株其他属细菌分别构建二元菌群降解芘和荧蒽,通过GC-MS测定HMW-PAHs降解率,并采用发光细菌法测定其降解前后的生物毒性. 结果表明,由PY97M和Marinobacter nanhaiticus D15-8WT组成的菌群(PY97M+D15-8W)对初始质量浓度均为0.1 g/L的单一碳源芘、荧蒽21 d后的降解率分别为67.40%和62.79%,相对于纯培养PY97M分别提高了20.30%和20.29%. 另外,该菌群对初始质量浓度均为0.1 g/L的芘和荧蒽组成的混合碳源14 d后的降解率分别为71.05%和67.36%;生物毒性检测结果显示,HMW-PAHs经该菌群降解后其急性毒性和遗传毒性相对于母体都有显著降低. 解环菌与海杆菌在芘和荧蒽的降解过程中表现出明显的协同效应,表明该菌群具有应用到HMW-PAHs污染海洋环境生物修复的潜力. 图5 表1 参24
Abstract:
In order to investigate the interaction between marine microorganisms and screen the synergic consortium in the biodegradation of high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs), we co-cultured HMW-PAHs degrader Cycloclasticus sp. PY97M with the other four bacterial species to construct defined consortia for pyrene and fluoranthene biodegradation. The degradation rates were measured by gas chromatography-mass spectrometry (GC-MS) and the end-product’s biotoxicity of PAHs measured by photobacterium test. Pyrene and fluoranthene, each at an initial concentration of 0.1 g/L, was supplemented as sole source of carbon and energy for the growth of the defined consortia throughout a 21-day incubation. The degradation rates by the consortium composed of Cycloclasticus sp. PY97M and Marinobacter nanhaiticus D15-8WT could reach 67.40% and 62.79% for pyrene and fluoranthene respectively, which was 20.30% and 20.29% higher than treatment of the PY97M pure culture. The consortium could utilize 71.05% of pyrene and 67.36% of fluoranthene respectively throughout a 14-day incubation, in which the mixed carbon source composed of pyrene and fluoranthene was each at an initial concentration of 0.1 g/L. The results of the biotoxicity test also indicated a significant reduction of the acute toxicity and geno-toxicity after HMW-PAHs biodegradation compared with the parent chemicals. The prominent synergic effect of Cycloclasticus strains and M. nanhaiticus strains suggested a great application potential in marine HMW-PAHs bioremediation. Fig 5, Tab 1, Ref 24

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备注/Memo

备注/Memo:
国家自然科学基金项目(40906062)和科技部项目(2012FY130300)资助 Supported by the National Natural Science Foundation of China (No. 40906062) and the Project of Ministry of Science and Technology of China (No. 2012FY130300)
更新日期/Last Update: 2013-08-22