|本期目录/Table of Contents|

[1]龙建友,罗定贵,陈永亨.一株Tl+抗性菌株的鉴定及吸附特性[J].应用与环境生物学报,2014,20(03):426-430.[doi:10.3724/SP.J.1145.2014.10004]
 LONG Jianyou,LUO Dinggui,CHEN Yongheng.Identification and biosorption characterization of a thallium-resistant strain[J].Chinese Journal of Applied & Environmental Biology,2014,20(03):426-430.[doi:10.3724/SP.J.1145.2014.10004]
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一株Tl+抗性菌株的鉴定及吸附特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年03期
页码:
426-430
栏目:
研究论文
出版日期:
2014-06-25

文章信息/Info

Title:
Identification and biosorption characterization of a thallium-resistant strain
作者:
龙建友罗定贵陈永亨
1广州大学环境科学与工程学院 广州 510006 2广东省放射性核素污染控制与资源化重点实验室 广州 510006
Author(s):
LONG Jianyou LUO Dinggui CHEN Yongheng
1College of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China 2Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, Guangzhou 510006, China
关键词:
抗Tl+菌菌株鉴定假单胞菌吸附特性废水处理
Keywords:
Tl+-resistant strain strain identification Pseudomonas biosorption characterization wastewater treatment
分类号:
X172
DOI:
10.3724/SP.J.1145.2014.10004
文献标志码:
A
摘要:
为了获得吸附铊(Tl)的功能菌株以应用于实际工业废水处理,从某矿区土壤中分离筛选出一株抗Tl+细菌BSA-68,对其进行了初步鉴定,并对其Tl+吸附特性和初步机理进行了研究. 16S rDNA序列分析表明,该菌株属于假单胞菌,命名为Pseudomonas sp. BSA-68(登录号:JF901709). 该菌株最大抗Tl+浓度为30 mg/L,溶液初始浓度、pH值、吸附时间、摇床转速及菌体生物量对Tl+吸附具有一定的影响,菌株在Tl+初始浓度20 g/L,吸附时间30 min,pH 6.0,摇床转速150 r/min,生物量2.0 g/L时,吸附效果最佳,在该条件下,BSA-68菌株对Tl+的吸附率可达93.77%. 红外光谱分析表明,该菌株细胞壁中的羟基、羧基及氨基是对Tl+吸附起主要贡献的官能团. 研究结果表明,BSA-68菌株可作为经济、高效、环境友好的生物材料进行Tl+废水处理.
Abstract:
This study aimed to obtain functional strains adsorbing thallium for application in industrial wastewater treatment. A thallium resistant bacterium BSA-68 strain was isolated from mining soil, preliminarily identified, and its Tl+ biosorption characteristics analyzed. The 16S rDNA sequence analysis revealed that the strain belonged to Pseudomonas sp. (accession number JF901709), therefore named as Pseudomonas sp. BSA-68. The strain possessed the capability of Tl+ resistance up to 30 mg/L in aqueous solution. The initial concentration of the solution, pH, adsorption time, speed and biomass all had certain degree of effect on Tl+ biosorption. The result showed that the optimum conditions for maximum biosorption were pH of 6.0 with biomass of 2.0 g/L, initial Tl+ concentration of 20 g/L, rotation 150 r/min and contact time of 30 min. Under such conditions, the adsorption rate could reach 93.77%. The Fourier transform infrared spectroscopy (FT-IR) analysis indicated that the main functional groups responsible for biosorption of thallium were hydroxyl, carboxyl and amino groups. The results presented in this study showed that the strain BSA-68 could be used as an effective, low cost biosorbent for the removal of thallium from waste water.

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备注/Memo

备注/Memo:
国家自然科学基金重点项目(40930743,41372248)、广东省科技计划项目(2010B030900008)和广州市教育局项目(2012A033)资助 Supported by the National Natural Science Foundation of China (40930743 & 41372248), the Science & Technology Plan of Guangdong, China (2010B030900008), and the Project of the Education Bureau of Guangzhou City (2012A033)
更新日期/Last Update: 2014-07-01