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[1]李强,李小林,黄文丽,等.基于rDNA ITS和ISSR标记研究四川松茸遗传多样性[J].应用与环境生物学报,2014,20(04):578-583.[doi:10.3724/SP.J.1145.2013.12032]
 LI Qiang,LI Xiaolin,HUANG Wenli,et al.Genetic diversity research of Sichuan Tricholoma matsutake based on rDNA-ITS sequencing and ISSR analysis[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):578-583.[doi:10.3724/SP.J.1145.2013.12032]
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基于rDNA ITS和ISSR标记研究四川松茸遗传多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年04期
页码:
578-583
栏目:
研究论文
出版日期:
2014-08-25

文章信息/Info

Title:
Genetic diversity research of Sichuan Tricholoma matsutake based on rDNA-ITS sequencing and ISSR analysis
作者:
李强 李小林 黄文丽 熊川 杨志荣 郑林用
1四川大学生命科学学院 成都 610065 2四川省农业科学院土壤肥料研究所 成都 610066 3四川省农业科学院生物技术核技术研究所 成都 610066
Author(s):
LI Qiang LI Xiaolin HUANG Wenli XIONG Chuan YANG Zhirong ZHENG Linyong
1College of Life Sciences, Sichuan University, Chengdu 610065, China 2Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 3Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
关键词:
松茸遗传资源ITS测序ISSR分子标记聚类分析地理分化
Keywords:
Tricholoma matsutake genetic resources ITS sequencing ISSR clustering analysis geographical differentiation
分类号:
S646.15+0.3
DOI:
10.3724/SP.J.1145.2013.12032
文献标志码:
A
摘要:
松茸是一种珍贵的食药用菌,至今无法人工栽培. 为更好地开发利用松茸资源,以采自四川小金、雅江、木里等7个县的24株不同生境的野生松茸子实体为材料,采用ISSR(Inter-Simple Sequence Repeat)分子标记及rDNA ITS序列分析的方法研究这些菌株的遗传资源及多样性. 从15条ISSR引物中筛选出2条多态性较好的引物对所有菌株进行了扩增,所得条带表明,在松茸中存在一定的遗传多样性;亲缘关系树状图表明,来自木里、会东、小金的大部分松茸样品聚为一个类群,而雅江、盐源、盐边、冕宁的为另一类群,呈现一定的地理分化现象. 通过对各菌株rDNA ITS序列分析表明,松茸菌株间的ITS序列相似性很高. 同时系统进化树表明四川松茸(Tricholoma matsutake)与美洲口蘑(T. magnivelare)同源关系较近,与欧洲口蘑(T. caligatum)距离较远,这与报道的形态学分类结果并不一致. 本研究表明四川松茸蕴含丰富的遗传信息. 图4 表4 参16
Abstract:
In order to understand the biological characteristics of Tricholoma matsutake, we studied its genetic resources and diversity. Twenty four wild fruit bodies of T. matsutake were collected from various sites in 7 counties including Xiaojin, Yajiang, Muli, Yanyuan, Yanbian, Huidong and Mianning of Sichuan Province. Their genetic diversity was investigated by ITS sequencing and ISSR analysis. The result showed that certain diversity was conserved in these strains, with the dendrogram of genetic relationships demonstrating the geographical distribution of T. matsutake in Sichuan as two clades, one from Muli, Huidong and Xiaojin, the other from Yajiang, Yanyuan, Yanbian and Mianning. In addition, T. matsutake in Sichuan showed high homology with T. magnivelare, but low homology with T. caligatum in phylogenetic analysis, which was not consistent with the morphological classification. This study showed that T. matsutake in Sichuan is rich in genetic information.

参考文献/References:

1 廖丽娟, 金光洙. 松茸的化学成分及其药理作用的研究进展[J]. 中国野生植物资源, 2010 (1): 12-14 [Liao LJ, Jin GZ. A review on chemistry and pharmacology of Tricholoma matsutake Sing [J]. Chin Wild Plant Resour, 2010 (1): 12-14]
2 Chen XQ, Wang QQ, Li JM. Comparison of simultaneous distillation and extraction, static headspace and headspace-solid phase microextraction coupled with GC/MS to measure the flavour components of Tricholoma matsutake [J]. Asian J Chem, 2013, 25 (11): 6059-6063
3 Ding X, Tang J, Cao M. Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake [J]. Int J Biol Macromol, 2010, 47 (2): 271-275
4 Yin XL, You QH, Jiang ZH. Immunomodulatory activities of different solvent extracts from Tricholoma matsutake (S. Ito et S. Imai) singer (higher basidiomycetes) on normal mice [J]. Int J Med Mush, 2012, 14 (6): 549-556
5 Hou YL, Ding X, Hou WR. Anti-microorganism, anti-tumor, and immune activities of a novel polysaccharide isolated from Tricholoma matsutake [J]. Pharmacogn Mag, 2013, 9 (35): 244-249
6 袁天凤, 段彬, 邱道持, 何江. 松茸的地理分布与生态研究[J]. 中国食用菌, 2006, 25 (4): 14-17 [Yuan TF, Duan B, Qiu DC, He J. The geographical distribution of Tricholoma matsutake [J]. Edible Fungi China, 2006, 25 (4): 14-17]
7 曹哲明. 松口蘑群的系统发育和生物地理学研究[D]. 北京: 中国科学院微生物研究所, 2003 [Cao ZM. Tricholoma matsutake group system development and biogeography research [D]. Beijing: Institute of Microbiology, Chinese Academy of Sciences, 2003]
8 陈姝, 钱正强, 宫霞, 赵榕, 杨明挚. SSR和RAPD 2种分子标记对昆明西山野生蘡薁葡萄资源遗传多样性分析比较[J]. 西南农业学报, 2010, 23 (6): 2008-2013 [Chen S, Qian ZQ, Gong X, Zhao R, Yang MZ. Comparison of SSR and RAPD technologies in genetic diversity analysis of wild grape resources Vitis bryoniaegolia from Xishan Mountain in Kunming [J]. SW China J Agric Sci, 2010, 23 (6): 2008-2013]
9 王波, 鲜灵. 金针菇菌株遗传多样性的ISSR分析[J]. 西南农业学报, 2013, 26 (4): 1593-1599 [Wang B, Xian L. Genetic diversity of Flammulina velutipes strains revealed by ISSR analysis [J]. SW China J Agric Sci, 2013, 26 (4): 1593-1599]
10 曹哲明, 姚一建. 松口蘑复合种形态学及生物地理学研究[J]. 菌物学报, 2004, 23 (1): 43-55 [Cao ZM, Yao YJ. Morphological and biogeographic study on Tricholoma matsutake-complex [J]. Mycosystema, 2004, 23 (1): 43-55]
11 王东东, 李良秋, 马连营, 陈爱枫, 吴清平. 大型真菌中SSR分子标记的开发与应用进展[J]. 微生物学通报, 2013, 40 (4): 646-654 [Wang DD, Li LQ, Ma LY, Chen AF, Wu QP. Progress in development and applications of SSR molecular marker in macrofungi [J]. Microbiology, 2013, 40 (4): 646-654]
12 李霞, 何涛, 淳泽. 石斛组培苗遗传差异性ISSR检测[J]. 应用与环境生物学报, 2013, 19 (2): 249-254 [Li X, He T, Chun Z. Detecting genetic diversity of Dendrobium tissue culture seedlings with ISSR method [J]. Chin J Appl Environ Biol, 2013, 19 (2): 249-254]
13 李维国, 常立民, 赵永光. 松茸半人工栽培的研究现状[J]. 微生物学杂志, 2002 (6): 49-51 [Li WG, Chang LM, Zhao YG. Partial artificial cultivation research of Tricholoma matsutake [J]. J Microbiol, 2002 (6): 49-51]
14 Murata H, Babasaki K. Intra- and inter-specific variations in the copy number of two types of retrotransposons from the ectomycorrhizal basidiomycete Tricholoma matsutake [J]. Mycorrhiza, 2005, 15 (5): 381-386
15 张微思, 朱萍, 王浚, 罗晓莉, 熊永生, 郭永红. 云南地区商品松茸ITS序列遗传多样性研究[J]. 中国食用菌, 2009 (5): 42-45 [Zhang WS, Zhu P, Wang J, Luo XL, Xiong YS, Guo YH. Study on genetic diversity of T. matsutake by ITS in Yunnan Province [J]. Edible Fungi China, 2009 (5): 42-45]
16 林戎斌, 张慧, 林陈强, 郑力, 陈济琛. ISSR、RAPD和SRAP分子标记技术在姬松茸菌株鉴定上的应用比较[J]. 福建农业学报, 2012, 27 (2): 149-152 [Lin RB, Zhang H, Lin CQ, Zheng L, Chen JC. DNA Fingerprinting and genetic diversity of cultivated strains of Agaricus blazei Murill [J]. Fujian J Agric Sci, 2012, 27 (2): 149-152]

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备注/Memo

备注/Memo:
四川省科技支撑计划项目(2013NZ0029,2012NZ0003,2014FZ0004)和四川省财政创新能力提升工程青年基金项目(2014CXSF-030)资助 Supported by the Science & Technology Pillar Project of Sichuan (2013NZ0029, 2012NZ0003 & 2014FZ0004), and the Youth Foundation for Innovational Capacity Building Project of Sichuan Financial Department (2014CXSF-030)
更新日期/Last Update: 2014-08-26