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[1]郭洪秀,张白梅,冯顺利,等.一株丁二酮高产菌株的鉴定及发酵条件优化[J].应用与环境生物学报,2015,21(01):41-45.[doi:10.3724/SP.J.1145.2014.03032]
 GUO Hongxiu,ZHANG Baimei,FENG Shunli,et al.Identification and fermentation optimization of a high butanedione yielding strain[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):41-45.[doi:10.3724/SP.J.1145.2014.03032]
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一株丁二酮高产菌株的鉴定及发酵条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年01期
页码:
41-45
栏目:
研究论文
出版日期:
2015-02-25

文章信息/Info

Title:
Identification and fermentation optimization of a high butanedione yielding strain
作者:
郭洪秀 张白梅 冯顺利 郭明月 徐青锐 曹 毅 乔代蓉
四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065
Author(s):
GUO Hongxiu ZHANG Baimei FENG Shunli GUO Mingyue XU Qingrui CAO Yi QIAO Dairong
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China
关键词:
丁二酮高产菌株植物乳杆菌发酵条件优化菌株鉴定单因素试验
Keywords:
butanedione high-yield strain Lactobacillus plantarum optimization of fermentation conditions strain identification single factor tests
分类号:
Q936 : TQ920.1
DOI:
10.3724/SP.J.1145.2014.03032
文献标志码:
A
摘要:
天然丁二酮是一种香精载体,为提高其产量,有必要筛选出丁二酮高产菌株及其最佳发酵条件. 从保存的一株丁二酮高产菌株6-1(2)出发,通过分子生物学方法对其进行鉴定,采用单因素试验和最佳单因素组合实验的方法筛选出该菌株的最佳发酵条件. 结果表明,实验菌株6-1(2)与植物乳杆菌Lactobacillus plantarum的AB326301.1序列同源性最高,初步鉴定为植物乳杆菌;发酵条件经过优化后,丁二酮的产量从初始的38 mg/L提高到167.56 mg/L,提高了340.95%,产量提高显著. 优化发酵条件为:牛肉膏10 g/L,柠檬酸氢二胺 2 g/L,酵母浸粉15 g/L,磷酸氢二钾2 g/L,乙酸钠2 g/L,葡萄糖20 g/L,蛋白胨30 g/L,吐温-80 1 mL/L,初始pH 6.2-6.4,接种量1.5%,37 ℃静置培养10 h;该发酵条件下的丁二酮产量提高显著. 本研究对丁二酮的工业化生产具有一定的参考价值.
Abstract:
Industrial production of butanedione is of great significance because the natural flavor carrier cannot meet the needs of people’s life. This research aimed to select a high butanedione yielding strain and to optimize its fermentation conditions for greater output of butanedione. Methods of molecular biology were used to identify the high butanedione yielding strain named 6-1(2); single factor tests and response surface methods were also used to determine its optimum fermentation conditions. The results showed that the experimental strain named 6-1(2) shared the highest homology with Lactobacillus plantarum AB326301.1 sequence, therefore determined as L. plantarum. Its optimum fermentation conditions were: beef paste 10 g/L, citric acid hydrogen diamine 2 g/L, yeast extract 15 g/L, potassium hydrogen phosphate 2 g/L, sodium acetate 2 g/L, glucose 20 g/L, peptone 30 g/L, twain-80 1 ml/L, the initial pH 6.2 - 6.4, the quantity of 1.5%, 37 ℃ stewing for 10 h. Under such fermentation conditions, the output of butanedione increased significantly for more than 3 times, from the original 38 mg/L to 167.56 mg/L. The results proved that the strain 6-1(2) can be high butanedione yielding in optimized fermentation conditions, which can be of great value for industrial butanedione production.

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备注/Memo

备注/Memo:
国家“十二五”科技支撑计划项目(2013BAD10B01,2014BAD02B02)、国家自然科学基金项目(31171447)和四川省科技厅项目(2014GZX0005,2013HH0007,2013GZX0161-3,2013GZ0058,2014JCPT005)资助 Supported by the Sci-tech Pillar Project of the Twelfth Five-year Plan of China (2013BAD10B01, 2014BAD02B02), the National Natural Science Foundation of China (31171447), and the Science and Technology Department of Sichuan Province (2014GZX0005, 2013HH0007, 2013GZX0161-3, 2013GZ0058, 2014JCPT005)
更新日期/Last Update: 2015-02-15