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[1]王文,杨波.硫脲对大豆苗期的毒理效应[J].应用与环境生物学报,2015,21(01):175-180.[doi:10.3724/SP.J.1145.2014.05023]
 WANG Wen,YANG Bo.Phytotoxicity of thiourea application on soybean seedlings[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):175-180.[doi:10.3724/SP.J.1145.2014.05023]
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硫脲对大豆苗期的毒理效应()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年01期
页码:
175-180
栏目:
研究简报
出版日期:
2015-02-25

文章信息/Info

Title:
Phytotoxicity of thiourea application on soybean seedlings
作者:
王文 杨波
三亚学院管理学院 三亚 572022
Author(s):
WANG WenYANG Bo
School of Management, Sanya University, Sanya 572022, China
关键词:
硫脲大豆生物量叶绿素酶活性
Keywords:
thiourea soybean biomass chlorophyll enzymes
分类号:
X592 : X173
DOI:
10.3724/SP.J.1145.2014.05023
文献标志码:
A
摘要:
通过盆栽实验,研究硝化抑制剂硫脲对大豆苗期的生物量、叶片金属离子含量、叶绿素含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和丙二醛(MDA)含量的影响. 结果表明,大豆的幼苗对土壤中硫脲的浓度较为敏感,低浓度硫脲(1.0 mmol kg-1)就能轻微抑制大豆的生长,当硫脲浓度达到5.0 mmol kg-1时,大豆幼苗的生长受到明显的抑制. 不同硫脲浓度种植条件下,大豆幼苗体内的铜离子和锌离子含量变化不大,而锰离子和铁离子含量却表现出明显差异. 锰离子含量随硫脲浓度的升高而升高,在高浓度硫脲(5.0 mmol kg-1)条件下由硫脲空白实验的18.58 mg kg-1上升到45.45 mg kg-1,增加了一倍以上. 而铁离子含量随硫脲浓度的升高而降低,在高浓度硫脲(5.0 mmol kg-1)条件下由硫脲空白实验的20.77 mg kg-1下降到17.63 mg kg-1. 高浓度的硫脲影响了大豆苗对铁的吸收,从而影响了叶绿素的合成,造成叶片叶绿素含量降低,由低浓度硫脲条件下最高的4.12 mg kg-1下降到2.15 mg kg-1. 研究结果还表明,低浓度硫脲能使大豆叶片中SOD、POD和CAT的活性提高,但当硫脲浓度升高时,其活性降低. 同时,当硫脲浓度达到5.0 mmol kg-1时,叶片MDA含量明显上升. 因此,大豆叶片中SOD、POD、CAT等酶的活性降低和MDA含量的上升表明大豆幼苗在高浓度硫脲种植条件下受到明显的环境胁迫和毒害作用.
Abstract:
For a precise evaluation of environmental and eco-toxicology effect of nitrification inhibitor on agroecosystem, the phytotoxicity of nitrification inhibitor should be fully understand. This paper aimed to study the phytotoxicity of thiourea and urea application on soybean seedlings. Effects of thiourea on the biomass, content of metal ions and chlorophyll level were studied in the leaves of soybean, and the effects of thiourea on anti-oxidative system (SOD, POD, and CAT) and content of MDA in the leaves of soybean were also investigated. The results showed that thiourea had great impact on the growth of soybean. Low concentration (1.0 mmol kg-1) of thiourea could slightly inhibit the growth of seedlings, and increase the activities of SOD, POD, CAT. Thiourea treatment of different concentrations had no effect on the content of Cu and Zn ion in soybean seedlings, but could obviously change the content of Mn and Fe ions in soybean seedlings. The content of Mn ion increased with the concentration of thiourea, from 18.58 mg kg-1 in the control to 45.45 mg kg-1 with 5 mmol kg-1 thiourea treatment. On the other hand, the content of Fe ion decreased with the concentration of thiourea, from 20.77 mg kg-1 in the control to 17.63 with 5 mmol kg-1 thiourea treatment. Heave inhibition effects and phytotoxic symptom were found in thriourea treatment of 5.0 mmol kg-1. The growth of seedlings was obviously inhibited; the content of chlorophyll in leaves of soybean decreased to 2.11 mg kg-1 compared to 3.56 mg kg-1 in that of the control. Simultaneously, the activities of SOD, POD, CAT and the content of MDA increased dramatically when thiourea reached 5.0 mmol kg-1. The heave inhibition effects and phytotoxic symptom in the soybean seedling indicated that thiourea could induce environmental stress in the seedlings.

参考文献/References:

1 巨晓棠, 张福锁. 中国北方土壤硝态氮的累积及其对环境的影响[J]. 生态环境, 2003, 12 (1): 24-28 [Ju XT, Zhang FS. Nitrate accumulation and its implication to environment in north China [J]. Ecol Environ, 2003, 12 (1): 24-28]
2 徐玉宏. 氮肥污染与防治[J]. 环境污染与防治, 2002, 24 (3): 174-175 [Xu YH. Pollution and control of nitrogen fertilizer [J]. Environ Pollut Control, 2002, 24 (3): 174-175]
3 Ju XT, Koua CL, Zhang FS, Christie P. Nitrogen balance and groundwater nitrate contamination: comparison among three intensive cropping systems on the north China plain [J]. Environ Pollut, 2006, 143 (1): 117-125
4 李俊良, 朱建华, 张晓晟, 孟祥霞, 陈清, 李晓林, 张福锁. 保护地番茄养分利用及土壤氮素淋失[J]. 应用与环境生物学报, 2001, 7 (2): 126-129 [Li JL, Zhu JH, Zhang XS, Meng XX, Chen Q, Li XL, Zhang FS. Nitrate leaching loss from soil and nutrient utilization by tomato in protected field [J]. Chin J Appl Environ Biol, 2001, 7 (2): 126-129]
5 孙爱文, 石元亮, 张德生, 郭爱民. 硝化/脲酶抑制剂在农业中的应用[J]. 土壤通报, 2004, 35 (3): 357-360 [Sun AW, Shi YL, Zhang DS, Guo AM. Application of nitrification-urease inhibitors in agriculture [J]. Chin J Soil Sci, 2004, 35 (3): 357-360]
6 徐星凯, 周礼恺, Oswald VC. 脲酶抑制剂/硝化抑制剂对土壤中尿素氮转化及形态分布的影响[J]. 土壤学报, 2000, 37 (3): 339-345 [Xu XK, Zhou LK, Oswald VC. Effect of urease/nitrification inhibitors on the distribution of transformed urea-N forms in soil [J]. Acta Pedol Sin, 2000, 37 (3): 339-345]
7 陈振华, 陈利军, 武志杰. 脲酶-硝化抑制剂对减缓尿素转化产物氧化及淋溶的作用[J]. 应用生态学报, 2005, 16 (2): 238-242 [Chen ZH, Chen LJ, Wu ZJ. Effects of urease and nitrification inhibitors on alleviating the oxidation and leaching of soil urea’s hydrolyzed product ammonium [J]. Chin J Appl Ecol, 2005, 16 (2): 238-242]
8 孙爱文, 石元亮, 郭爱民, 张德生. 硫脲在农业中的研究和应用[J].土壤通报, 2003, 34 (4): 377-380 [Sun AW, Shi YL, Guo AM, Zhang DS. Research an application of thiourea in agriculture [J]. Chin J Soil Sci, 2003, 34 (4): 377-380]
9 倪秀菊, 李玉中, 徐春英, 李巧珍. 土壤脲酶抑制剂和硝化抑制剂的研究进展[J]. 中国农学通报, 2009, 25 (12): 145-149 [Ni XJ, Li YZ, Xu CY, Li QZ. Advance of research on urease inhibitor and nitrification inhibitor in soil [J]. Chin Agric Sci Bull, 2009, 25 (12): 145-149]
10 余光辉, 张杨珠, 万大娟. 几种硝化抑制剂对土壤和小白菜硝酸盐含量及产量的影响[J]. 应用生态学报, 2006, 17 (2): 247-250 [Xu GH, Zhang YZ, Wan DJ. Effects of nitrification inhibitors on nitrate content in soil and pakehoi and on pakchoi yield [J]. Chin J Appl Ecol, 2006, 17 (2): 247-250]
11 Saha MP. Effect of thiourea, thiamine and ascorbic acid on growth and yield of maize (Zea mays L.) [J]. J Agron Crop Sci, 1993, 171 (1): 65-69
12 Vidal PI, Longeeri SL. Use of nitrification inhibitors, nitrapyrin and thiourea on spring sown wheat [J]. Agric Tecnia, 1988, 48 (2): 137-141
13 李合生. 植物生理生化实验原理与技术[M]. 北京: 中国高等教育出版社, 2007 [Li HS. Experimental principle of plant physiology and biochemistry [M]. Beijing: China Higher Education Press, 2007]
14 李宏文, 史绮, 曹阳, 钱锟. 紫外光对几种水生植物过氧化氢酶 (CAT) 活性的影响[J]. 环境科学, 1993, 14 (4): 74-77 [Li HW, Shi Q, Cao Y, Qian K. The effects of the UV light on the Catalase (CAT) activity of several species of aquatic plants [J]. Chin J Environ Sci, 1993, 14 (4): 74-77]
15 汤章城. 现代植物生理学实验指南[M]. 北京: 科学出版社, 1999 [Tang ZC. Modern plant physiology experiment [M]. Beijing: Science Press, 1999]
16 孙爱文, 石元亮, 朱志峰, 尹宏斌. 硫脲及抑制剂组合对土壤尿素氮转化和玉米产量的影响[J]. 植物营养与肥料学报, 2005, 11 (4): 481-486 [Sun AW, Shi YL, Zhu ZF, Yin HB. Effect of combination of thiourea and inhibitors on soil urea-N transformation and corn yield [J]. Plant Nutr Fert Sci, 2005, 11 (4): 481-486]
17 祁桂林, 陈平, 黄庆. 硫脲对水稻幼苗生长和部分生理指标的影响[J]. 中国农学通报, 2006, 22 (1): 156-157 [Qi GL, Chen P, Huang Q. Effects of thiourea on growth and some physiological character of rice seedling [J]. Chin Agric Sci Bull, 2006, 22 (1): 156-157]
18 王煌平, 何盈, 张青, 张晓玲, 罗涛, 蔡顺香. 硝化抑制剂及其组合对小白菜产量, 品质的影响与土壤残留分析[J]. 福建农业学报, 2009, 24 (5): 580-585 [Wang HP, He Y, Zhang Q, Zhang XL, Luo T, Cai SX. Effect of nitrification inhibitors and their combinations on yield and quality of Brassica chinensis and analysis of their inhibitor residue in soil [J]. Fujian J Agric Sci, 2009, 24 (5): 580-585]
19 Xiong XJ, Makoto H, Hirokazu T, Lee MG, Tadashi H. Analysis of acclimation behavior against nitrification inhibitors in activated sludge processes [J]. J Ferment Bioeng, 1998, 86 (2): 207-214
20 刘鑫, 朱端卫, 雷宏军, 耿明建, 周文兵. 酸性土壤活性锰与pH, Eh 关系及其生物反应[J]. 植物营养与肥料学报, 2003, 9 (3): 317-323 [Liu X, Zhu DW, Lei HJ, Geng JM, Zhou WB. Dynamic relationship between soil active Mn and pH, Eh in acid soils and its biological response [J]. Plant Nutr Fert Sci, 2003, 9 (3): 317-323]
21 任立民, 刘鹏. 锰毒及植物耐性机理研究进展[J].生态学报, 2007, 27 (1): 357-367 [Ren LM, Liu P. Review of manganese toxicity and the mechanisms of plant tolerance [J]. Acta Ecol Sin, 2007, 27 (1): 357-367]
22 钟鹏, 朱占林, 李志刚, 王建丽, 张玉玲. 干旱和低磷胁迫对大豆叶保护酶活性的影响[J]. 中国农学通报, 2005, 21 (2): 153-154 [Zhong P, Zhu ZL, Li ZG, Wang JL, Zhang YL. Effects of low-phosphorus and drought stresses on protective enzyme activities of soybean [J]. Chin Agric Sci Bull, 2005, 21 (2): 153-154]
23 Gao S, Ouyang C, Wang S, Xu Y, Tang L, Chen F. Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings [J]. Plant Soil Environ, 2008, 54 (9): 374-381
24 王红梅, 包维楷, 李芳兰. 不同干旱胁迫强度下白刺花幼苗叶片的生理生化反应[J]. 应用与环境生物学报, 2008, 14 (6): 757-762 [Wang HM, Bao WK, Li FL. Physiological and biochemical responses of two-years0old Sophora davidii seedling leaves to different water stress [J]. Chin J Appl Environ Biol, 2008, 14 (6):757-762]
25 尹永强, 胡建斌, 邓明军. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J]. 中国农学通报, 2007, 23 (1): 105-110 [Yin YQ, Hu JB, Deng MJ. Latest development of antioxidant system and responses to stress in plant leaves [J]. Chin Agric Sci Bull, 2007, 23 (1): 105-110]
26 Jonaliza CL, Grenggrai P, Boonrat J, Theerayut T. Quantitative trait loci associated with drought tolerance at reproductive stage in rice [J]. Plant Phys, 2004, 135 (1): 384-399
27 杨海燕, 施国新, 徐秦松, 计汪栋, 王红霞, 李阳. Cd2+胁迫对竹叶眼子菜的毒理学效应分析[J]. 应用与环境生物学报, 2008, 14 (3): 366-370 [Yang HY, Shi GX, Xu QS, Ji WD, Wanh HX, Li Y. Phytotoxicity of Cd2+ on leaf cells of potamogeton malaianus [J]. Chin J Appl Environ Biol, 2008, 14 (3): 366-370]
28 Chaves MM, Oliveira MM. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture [J]. J Exp Bot, 2004, 55: 1455-1459
29 孙天国, 沙伟, 刘岩. 复合重金属胁迫对两种薛类植物生理特性的影响[J]. 生态学报, 2010, 30 (9): 2332-2339 [Sun TG, Sha W, Liu Y. The effects of compound heavy metal stress on some physiological characteristics of two moss species [J]. Acta Ecol Sin, 2010, 30 (9): 2332-2339]
30 张义贤, 张丽萍. 重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响[J]. 农业环境科学学报, 2006, 25 (4): 857-860 [Zhang YX, Zhang LP. Effects of Heavy Metals on membrane lipid peroxidation, proline and soluble sugar in roots of Hordeum vulgare [J]. J Agro-Environ Sci, 2006, 25 (4): 857-860]

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备注/Memo

备注/Memo:
海南省高等学校科研项目(Hjkj2013-46)、国家自然科学基金项目(30770389)和三亚学院人才引进项目资助 Supported by the Colleges and Universities Scientific Research Project of Hainan Province (Hjkj2013-46), the National Natural Science Foundation of China (30471378) and the Talent-Recruiting Program of Sanya University
更新日期/Last Update: 2015-02-15