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[1]沈林松,张 颖,张伟妮,等.果糖对莆田黑猪精液中NAGase的抑制动力学[J].应用与环境生物学报,2015,21(01):46-50.[doi:10.3724/SP.J.1145.2014.07013]
 SHEN Linsong,ZHANG Ying,ZHANG Weini,et al.Inhibitory kinetics of fructose on NAGase in semen of Putian black pigs[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):46-50.[doi:10.3724/SP.J.1145.2014.07013]
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果糖对莆田黑猪精液中NAGase的抑制动力学()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年01期
页码:
46-50
栏目:
研究论文
出版日期:
2015-02-25

文章信息/Info

Title:
Inhibitory kinetics of fructose on NAGase in semen of Putian black pigs
作者:
沈林松 张 颖 张伟妮 赖育河 黄小红
福建农林大学中西兽医结合与动物医学保健福建省高校重点实验室 福州 350002
Author(s):
SHEN Linsong ZHANG Ying ZHANG Weini LAI Yuhe HUANG Xiaohong
Fujian Higher Education Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
果糖莆田黑猪精液N-乙酰-β-D-氨基葡萄糖苷酶抑制动力学
Keywords:
fructose Putian black pig semen N-Acetyl-β-D-glucosaminidase inhibitory kinetics
分类号:
Q556.2 : S828.34
DOI:
10.3724/SP.J.1145.2014.07013
文献标志码:
A
摘要:
为了解精液中不同类型糖类物质对精子细胞中N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)的影响,以莆田黑猪精液NAGase为材料,研究精液和精液稀释液主要成分之一果糖对酶的抑制作用. 结果显示,果糖对NAGase具有浓度效应(IC50 = 575 mmol L-1). 果糖对NAGase的抑制类型是可逆的非竞争性抑制,抑制常数是1.27 × 10-2 mmol L-1. 应用底物反应的动力学方法研究果糖对该酶的抑制动力学,建立了抑制动力学模型,证明了果糖对该酶的影响是快速结合再缓慢失活的过程. 本研究表明,在高浓度的抑制剂溶液中NAGase将完全失活,底物对其无保护作用.
Abstract:
This research aimed to understand the effects of different types of sugar on the N-Acetyl-β-D-glucosaminidase (EC 3.2.1.52, NAGase) in sperm cells. NAGase from semen of Putian black pigs was used as the material to study the effects of fructose, one of the main components of the sperm and semen dilutions. The inhibition kinetics of fructose on the enzyme model was set up by using the kinetic method of substrate reaction. The results showed that NAGase was dose-dependent on fructose (IC50 = 575 mmol L-1) and the inhibition type of fructose on NAGase was reversible noncompetitive inhibition, with the inhibition constant as 1.27 × 10-2 mmol L-1. The process of fructose on the enzyme was first slow deactivation then rapid combination. These results suggested that the enzyme is completely deactivated in fructose solution of high concentration, where the substrate has no protection to the enzyme.

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备注/Memo

备注/Memo:
福建省科技计划项目重点项目(2012N0002)资助 Support by the Science and Technology Major Project of Fujian Province (2012N0002)
更新日期/Last Update: 2015-02-15