|本期目录/Table of Contents|

[1]秦改娟,王珊珊,陈青君,等.一株假芝的分离鉴定与生物学特性[J].应用与环境生物学报,2015,21(03):464-469.[doi:10.3724/SP.J.1145.2014.11015]
 QIN Gaijuan,WANG Shanshan,CHEN Qingjun,et al.Isolation, identification, and biological characteristics of a wild Amauroderma mushroom[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):464-469.[doi:10.3724/SP.J.1145.2014.11015]
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一株假芝的分离鉴定与生物学特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年03期
页码:
464-469
栏目:
研究论文
出版日期:
2015-06-25

文章信息/Info

Title:
Isolation, identification, and biological characteristics of a wild Amauroderma mushroom
作者:
秦改娟王珊珊陈青君张昊琳张国庆
1北京农学院生物科学与工程学院,农业部都市农业(北方)重点实验室 北京 102206 2北京农学院植物科学技术学院 北京 102206
Author(s):
QIN Gaijuan WANG Shanshan CHEN Qingjun ZHANG Haolin ZHANG Guoqing
1Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China 2College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China
关键词:
假芝形态鉴定ITS鉴定培养条件
Keywords:
Amauroderma subrugosum morphological identification ITS identification culture conditions
分类号:
Q949.329.75
DOI:
10.3724/SP.J.1145.2014.11015
文献标志码:
A
摘要:
灵芝是我国著名传统药用真菌,假芝属(Amauroderma)真菌作为灵芝科重要属,我国已报道种共有22个. 本研究从广州白云山采集到一株野生假芝子实体,经组织分离获得菌丝体纯培养,编号GZ-01菌株. 利用形态鉴定和ITS鉴定手段,确定其分类学地位为假芝属,系统命名为A. subrugosum,为中国首次记录种,菌种保藏号ACCC52954. 生物学特性研究表明,菌丝生长的最适碳源为甘露醇,最适氮源为酵母浸膏或大豆蛋白胨,最适C/N比为20/1,最适生长因子为维生素B6,最适生长温度为28 ℃,最适pH值范围为6.0-7.5. 本研究获得新的药用真菌资源,其培养条件研究可为进一步开发利用奠定实验基础.
Abstract:
Reishi mushrooms are Chinese traditional medicinal fungi with antioxidant, antitumor, immunomodulatory activities. Amauroderma is one of the important genera in the family Ganodermataceae. All together 22 Amauroderma species have been reported in China. This study isolated a pure cultured strain GZ-01 from mushroom fruiting bodies collected in the Baiyun Mountain, Guangzhou Province. Based on morphological and ITS identification methods, it was identified as Amauroderma subrugosum with the collection No. of ACCC52954. It is a new record of the genus Amauroderma in China based on morphological and molecular identification. Culture conditions study showed the optimum condition for the myceliao growth included mannitol as the carbon source, yeast extract or soy peptone as nitrogen source, the C/N rate as 20/1, growth factor of Vitamin B6, temperature of 28 oC, and the pH range as 6.0-7.5. The results show that the fungus is a newly reported medicinal fungal species, which is an important reference for further artificial domestic cultivation and applications of the mushroom resource.

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备注/Memo

备注/Memo:
国家自然科学基金项目(31200070)、北京市科技新星计划项目(xx2015B025)、北京高等学校青年英才计划项目(YETP1714)和北京市本科生科学研究计划项目资助 Supported by the National Natural Science Foundation of China (31200070), the Beijing NOVA Program (xx2015B025), the Beijing Higher Education Young Elite Teacher Project (YETP1714) and the Supporting Project of the Undergraduate Research Program of Beijing
更新日期/Last Update: 2015-06-23