|本期目录/Table of Contents|

[1]张举龙,陈青君,秦岭,等.油松林地点柄乳牛肝菌菌塘细菌群落动态[J].应用与环境生物学报,2015,21(05):974-980.[doi:10.3724/SP.J.1145.2015.03001]
 ZHANG Julong,CHEN Qingjun,QIN Ling,et al.Dynamics of bacterial community of Suillus granulatus mushroom shiro in Pine (Pinus tabuliformis) forests[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):974-980.[doi:10.3724/SP.J.1145.2015.03001]
点击复制

油松林地点柄乳牛肝菌菌塘细菌群落动态()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年05期
页码:
974-980
栏目:
研究论文
出版日期:
2015-10-25

文章信息/Info

Title:
Dynamics of bacterial community of Suillus granulatus mushroom shiro in Pine (Pinus tabuliformis) forests
作者:
张举龙 陈青君 秦岭 王珊珊 张国庆
1北京农学院植物科学技术学院农业应用新技术北京市重点实验室 北京 102206 2北京农学院生物科学与工程学院农业部都市农业(北方)重点实验室 北京 102206
Author(s):
ZHANG Julong CHEN Qingjun QIN Ling WANG Shanshan ZHANG Guoqing
1College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206 China 2College of Biological Science and Engineering, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206 China
关键词:
点柄乳牛肝菌16S rDNAPCR-DGGE细菌群落出菇期
Keywords:
Suillus granulatus 16S rDNA PCR-DGGE bacterial community fruiting period
分类号:
S714.343.6
DOI:
10.3724/SP.J.1145.2015.03001
文献标志码:
A
摘要:
为了解北京山区油松(Pinus tabuliformis)林地点柄乳牛肝菌(Suillus granulatus)出菇期间菌塘内的细菌群落变化规律,收集出菇期前后(5-11月)的菌塘土样,通过聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)方法进行评估与分析. 利用土壤总DNA提取试剂盒提取不同时期总DNA,以细菌通用引物338F和518R进行PCR扩增和DGGE,进一步对细菌群落的DGGE指纹图谱分析后进行切胶回收和测序. 结果显示在点柄乳牛肝菌出菇期间,菌塘中细菌群落较为丰富,群落多样性指数维持在较高水准,并且菌塘中的细菌群落呈现出季节性变化. 菌塘细菌分属于5个门(酸杆菌门、放线菌门、拟杆菌门、厚壁菌门和变形菌门)22个属(酸杆菌属、氨基酸杆菌属等). 菌塘中变形菌门和酸杆菌门菌群的相对丰度较高,为优势菌群,其中α-变形菌纲为菌塘中最丰富的菌群. γ-变形菌纲菌群的相对丰度变化与点柄乳牛肝菌子实体发生周期一致. 酸杆菌门菌群的相对丰度在点柄乳牛肝菌出菇前期(7月)明显增大. 这些发现为点柄乳牛肝菌野生资源保育与人工驯化等后续研究提供了理论和实验依据.
Abstract:
To evaluate bacterial community variation in the mushroom shiro of Suillus granulatus during fruiting, we collected soil samples from the mushroom shiro in the pine (Pinus tabuliformis) forest of mountainous area in Beijing from May to November and evaluated the bacterial community using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE). Total soil DNA was extracted using a commercial soil DNA isolation kit. PCR amplification and DGGE were performed using bacterial universal primers 338F and 518R. The specific bands were excised from the gel and sequenced. The results revealed that soil bacterial community maintained considerably high level and changed seasonally with the mushroom fruiting. In total, 53 bands of DGGE profiles were sequenced and divided into 5 phyla (Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria and 22 genera (Acidobacterium, Aminobacter, et al). Species from Proteobacteria and Acidobacteria were the dominant bacterial groups sharing considerably high relative abundance, while class α-Proteobacteria was the most abundant group. The variation of the relative abundance of γ-Proteobacteria species was consistent with the mushroom fruiting season. The relative abundance of Acidobacteria species obviously increased before mushroom flush (in July). The fruiting of S. granulatus and the relative abundance of γ-Proteobacteria were correlated with each other. The present study provided a basis for conservation and domestication of mushroom S. granulatus.

参考文献/References:

1 Kivlin SN, Hawkes CV, Treseder KK. Global diversity and distribution of arbuscular mycorrhizal fungi [J]. Soil Biol Biochem, 2011, 43 (11): 2294-2303
2 Brundrett M. Diversity and classification of mycorrhizal associations [J]. Biol Rev Camb Philos Soc, 2004, 79 (3): 473-495
3 Toju H, Sato H, Tanabe AS. Diversity and spatial structure of belowground plant-fungal symbiosis in a mixed subtropical forest of ectomycorrhizal and arbuscular mycorrhizal plants [J]. PloS ONE, 2014, 9 (1): e86566
4 Mongodin EF, Emerson JB, Nelson KE. Microbial metagenomics [J]. Genome Biol, 2005, 6 (10): 347
5 Le Chatelier E, Nielsen T, Qin J, Prifti E, Hildebrand F, Falony G, Almeida M, Arumugam M, Batto JM, Kennedy S, Leonard P, Li J, Burgdorf K, Grarup N, Jorgensen T, Brandslund I, Nielsen HB, Juncker AS, Bertalan M, Levenez F, Pons N, Rasmussen S, Sunagawa S, Tap J, Tims S, Zoetendal EG, Brunak S, Clement K, Dore J, Kleerebezem M, Kristiansen K, Renault P, Sicheritz-Ponten T, de Vos WM, Zucker JD, Raes J, Hansen T, Meta HITc, Bork P, Wang J, Ehrlich SD, Pedersen O. Richness of human gut microbiome correlates with metabolic markers [J]. Nature, 2013, 500 (7464): 541-546
6 DeLong EF. Microbial community genomics in the ocean [J]. Nat Rev Microbiol, 2005, 3 (6): 459-469
7 Vik U, Logares R, Blaalid R, Halvorsen R, Carlsen T, Bakke I, Kolsto AB, Okstad OA, Kauserud H. Different bacterial communities in ectomycorrhizae and surrounding soil [J]. Sci Rep-UK, 2013, 3: 3471
8 Yang Q, Wang J, Wang H, Chen X, Ren S, Li X, Xu Y, Zhang H, Li X. Evolution of the microbial community in a full-scale printing and dyeing wastewater treatment system [J]. Bioresour Technol, 2012, 117: 155-163
9 Werner JJ, Knights D, Garcia ML, Scalfone NB, Smith S, Yarasheski K, Cummings TA, Beers AR, Knight R, Angenent LT. Bacterial community structures are unique and resilient in full-scale bioenergy systems [J]. Proc Natl Acad Sci USA, 2011, 108 (10): 4158-4163
10 Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA [J]. Appl Environ Microbiol, 1993, 59 (3): 695-700
11 Anderson IC, Cairney JW. Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques [J]. Environ Microbiol, 2004, 6 (8): 769-779
12 Carvalhais LC, Dennis PG, Tyson GW, Schenk PM. Application of metatranscriptomics to soil environments [J]. J Microbiol Meth, 2012, 91 (2): 246-251
13 Dick GJ, Andersson AF, Baker BJ, Simmons SL, Thomas BC, Yelton AP, Banfield JF. Community-wide analysis of microbial genome sequence signatures [J]. Genome Biol, 2009, 10 (8): R85
14 Ercolini D. PCR-DGGE fingerprinting: novel strategies for detection of microbes in food [J]. J Microbiol Meth, 2004, 56 (3): 297-314
15 Dias ES, Araujo EF, Guimaraes WV, Muchovej RM. Production and regeneration of protoplasts from the mycorrhizal fungus Suillus granulatus [J]. World J Microb Biot, 1996, 12 (6): 625-628
16 Nara K. Ectomycorrhizal networks and seedling establishment during early primary succession [J]. New Phytol, 2006, 169 (1): 169-178
17 Rincon A, Ruiz-Diez B, Garcia-Fraile S, Garcia JA, Fernandez-Pascual M, Pueyo JJ, de Felipe MR. Colonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus [J]. FEMS Microbiol Ecol, 2005, 51 (3): 303-311
18 Li X, Jin X, Li Qiang, Xiong C, Zheng L. Effects of ecological environment factors on soil microorganisms of Tricholoma matsutake shiro in Sichuan Province [J]. Chin J Appl Environ Biol, 2015, 21 (1): 164-169
19 Ogawa M. Microbial ecology of “shiro” in Tricholoma matsutake (Ito. et Imai) Sing. and its allied species. V Tricholoma matsutake in Tsuga sieboldii forests [J]. Trans Mycol Soc Jpn, 1977, 18,: 34-36
20 Chen QJ, Niu YR, Hao C, Zhang GQ, Qin L, Li JH, Liu S, Wei HL. Occurrence of edible mycorrhizal mushroom in the pine forests of Beijing mountainous area [J]. Sci Agric Sin, 2011, 44 (16): 3377-3385
21 Zeng G, Yu Z, Chen Y, Zhang J, Li H, Yu M, Zhao M. Response of compost maturity and microbial community composition to pentachlorophenol (PCP)-contaminated soil during composting [J]. Bioresour Technol, 2011, 102 (10): 5905-5911
22 Schabereiter-Gurtner C, Maca S, Kaminsky S, Rolleke S, Lubitz W, Barisani-Asenbauer T. Investigation of an anaerobic microbial community associated with a corneal ulcer by denaturing gradient gel electrophoresis and 16S rDNA sequence analysis [J]. Diagn Microb Infec Dis, 2002, 43 (3): 193-199
23 Garbeva P, van Veen JA, van Elsas JD. Microbial diversity in soil: selection microbial populations by plant and soil type and implications for disease suppressiveness [J]. Annu Rev Phytopathol, 2004, 42: 243-270
24 Kirk JL, Beaudette LA, Hart M, Moutoglis P, Klironomos JN, Lee H, Trevors JT. Methods of studying soil microbial diversity [J]. J Microbiol Meth, 2004, 58 (2): 169-188
25 Zhao F, Xu K. Microbial genetic diversity and ciliate community structure along an environmental gradient in coastal soil [J]. Eur J Protistol, 2013, 49 (4): 516-525
26 Wang P, Liu Y, Yin Y, Jin H, Wang S, Xu F, Zhao S, Geng X. Diversity of microorganisms isolated from the soil sample surround Chroogomphus rutilus in the Beijing region [J]. Int J Biol Sci, 2011, 7 (2): 209-220
27 Lee KB, Liu CT, Anzai Y, Kim H, Aono T, Oyaizu H. The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov [J]. Int J Syst Evol Microb, 2005, 55 (Pt 5): 1907-1919
28 Asakawa S, Kimura M. Comparison of bacterial community structures at main habitats in paddy field ecosystem based on DGGE analysis [J]. Soil Biol Biochem, 2008, 40: 1322-1329
29 Gelsomino A, Cacco G. Compositional shifts of bacterial groups in a solarized and amended soil as determined by denaturing gradient gel electrophoresis [J]. Soil Biol Biochem, 2006, 38: 91-102
30 Xu Y, Wang G, Jin J, Liu J, Zhang Q, Liu X. Bacterial communities in soybean rhizosphere in response to soil type, soybean genotype, and their growth stage [J]. Soil Biol Biochem, 2009, 41: 919-925
31 Niu Y, Wang M, Zhang G, Chen Q, Liu S, Jiang W. Its sequence analysis of strain obtained from wild Suillus granulatus fruit bodies and optimization of selected parameters [J]. J Edible Fungi, 2009, 20 (4): 6-10
32 Barns SM, Cain EC, Sommerville L, Kuske CR. Acidobacteria phylum sequences in uranium-contaminated subsurface sediments greatly expand the known diversity within the phylum [J]. Appl Environ Microbiol, 2007, 73 (9): 3113-3116
33 Quaiser A, Ochsenreiter T, Lanz C, Schuster SC, Treusch AH, Eck J, Schleper C. Acidobacteria form a coherent but highly diverse group within the bacterial domain: evidence from environmental genomics [J]. Mol Microbiol, 2003, 50 (2): 563-575
34 Eichorst SA, Breznak JA, Schmidt TM. Isolation and characterization of soil bacteria that define Terriglobus gen. nov., in the phylum Acidobacteria [J]. Appl Environ Microbiol, 2007, 73 (8): 2708-2717
35 Kuske CR, Barns SM, Busch JD. Diverse uncultivated bacterial groups from soils of the arid southwestern United States that are present in many geographic regions [J]. Appl Environ Microbiol, 1997, 63 (9): 3614-3621
36 Rappe MS, Giovannoni SJ. The uncultured microbial majority [J]. Annu Rev Microbiol, 2003, 57: 369-394

相似文献/References:

[1]苏莹 陈莉 汪辉 刘兆普.海洋石油降解菌的筛选与降解特性[J].应用与环境生物学报,2008,14(04):518.
[2]洪璇,张晓君,孟智奇,等.废水反硝化生物反应器中喹啉降解细菌的分离与特性[J].应用与环境生物学报,2008,14(06):803.[doi:10.3724/SP.J.1145.2008.00803]
 HONG Xuan,ZHANG Xiaojun**,MENG Zhiqi,et al.Isolation and Characteristics of Quinoline Degrading Bacteria from Wastewater Denitrifying Bioreactor[J].Chinese Journal of Applied & Environmental Biology,2008,14(05):803.[doi:10.3724/SP.J.1145.2008.00803]
[3]付博,王卫卫,郝莹,等.紫花苜蓿根际氢氧化细菌的分离与鉴定[J].应用与环境生物学报,2009,15(05):650.[doi:10.3724/SP.J.1145.2009.00650]
 FU Bo,WANG Weiwei,HAO Ying,et al.Isolation and Identification of Hydrogen-oxidizing Bacteria in Medicago sativa Rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):650.[doi:10.3724/SP.J.1145.2009.00650]
[4]张晓霞,马晓彤,曹卫东,等.紫云英根瘤菌的系统发育多样性[J].应用与环境生物学报,2010,16(03):380.[doi:10.3724/SP.J.1145.2010.00380]
 ZHANG Xiaoxia,MA Xiaotong,CAO Weidong,et al.Phylogenetic Diversity of Rhizobial Bacteria Isolated from Astragalus sinicus[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):380.[doi:10.3724/SP.J.1145.2010.00380]
[5]袁敏,张辉,胡国全.一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析[J].应用与环境生物学报,2010,16(05):705.[doi:10.3724/SP.J.1145.2010.00705]
 YUAN Min,ZHANG Hui,HU Guoquan.Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):705.[doi:10.3724/SP.J.1145.2010.00705]
[6]陈法霖,张凯,郑华,等.PCR-DGGE技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响[J].应用与环境生物学报,2011,17(02):145.[doi:10.3724/SP.J.1145.2011.00145]
 CHEN Falin,ZHANG Kai,ZHENG Hua,et al.Analyzing the Effect of Mixed Decomposition of Conifer and Broadleaf Litters on Soil Microbial Communities by Using PCR-DGGE[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):145.[doi:10.3724/SP.J.1145.2011.00145]
[7]吕早生,关海燕,李凌凌,等.一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定[J].应用与环境生物学报,2011,17(03):326.[doi:10.3724/SP.J.1145.2011.00326]
 LÜ,Zaosheng,GUAN Haiyan,et al.Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):326.[doi:10.3724/SP.J.1145.2011.00326]
[8]郭娜,李志敏,叶勤.烷烃降解菌的筛选、鉴定及优势菌株的降解特性[J].应用与环境生物学报,2011,17(04):572.[doi:10.3724/SP.J.1145.2011.00572]
 GUO Na,LI Zhimin,YE Qin.Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):572.[doi:10.3724/SP.J.1145.2011.00572]
[9]刘力嘉,马玉龙,谢丽,等.泰乐菌素降解菌的分离鉴定及其系统发育分析[J].应用与环境生物学报,2012,18(06):1038.[doi:10.3724/SP.J.1145.2012.01038]
 LIU Lijia,MA Yulong,XIE Li,et al.Isolation, Identification and Phylogenetic Analysis of a Tylosin-degrading Strain[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):1038.[doi:10.3724/SP.J.1145.2012.01038]
[10]陈丹丹,顾胜华,张金娜,等.肠道菌群对免疫系统的影响及其群落分析方法[J].应用与环境生物学报,2013,19(03):542.[doi:10.3724/SP.J.1145.2013.00542]
 CHEN Dandan,GU Shenghua,ZHANG Jinna,et al.Effect of Intestinal Microbes on the Immune System and the Latest Research Methods[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):542.[doi:10.3724/SP.J.1145.2013.00542]

备注/Memo

备注/Memo:
国家自然科学基金项目(31200070)、国家“十二五”科技支撑计划项目(2012BAD14B09)、北京市科技新星计划(xx2015B025)、北京高等学校青年英才计划项目(YETP1714)和现代农业产业技术体系项目资助 Supported by the National Natural Science Foundation of China (31200070), the Key Sci-tech Project of the Twelfth Five Year Plan of China (2012BAD14B09), the Beijing NOVA Program (XX2015B025), the Beijing Higher Education Young Elite Teacher Project (YETP1714), and the Beijing Innovative Grant of Modern Agricultural Technology System (PXM2013-014207-000096)
更新日期/Last Update: 2015-10-29