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[1]王淑芳,汪晶晶,王军强,等.海洋生防细菌在黄瓜根际土壤的定殖规律[J].应用与环境生物学报,2015,21(06):1095-1099.[doi:10.3724/SP.J.1145.2015.03032]
 WANG Shufang,WANG Jingjing,WANG Junqiang,et al.The colonization rule of marine biocontrol bacteria around cucumber rhizosphere[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1095-1099.[doi:10.3724/SP.J.1145.2015.03032]
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海洋生防细菌在黄瓜根际土壤的定殖规律()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1095-1099
栏目:
研究论文
出版日期:
2015-12-25

文章信息/Info

Title:
The colonization rule of marine biocontrol bacteria around cucumber rhizosphere
作者:
王淑芳 汪晶晶 王军强 王琦 马桂珍 李世东 孙漫红 暴增海
1淮海工学院海洋学院 连云港 222005 2中国农业科学研究院植物保护研究所 北京 100081
Author(s):
WANG Shufang WANG Jingjing WANG Junqiang WANG Qi MA Guizheng LI Shidong SUN Manhong BAO Zenghai
1Marine College, Huaihai Institute of Technology, Lianyungang 222005, China 2Institute of Plant Protection, CAAS, Beijing 100081, China
关键词:
生物防治根际定殖温度pH值有机质含量多粘类芽孢杆菌实时荧光定量PCR技术
Keywords:
biological control rhizosphere colonization temperature pH value organic matter content Paenibacillus polymyxa Real-time fluorescence quantitative-PCR
分类号:
S476 : S154.3
DOI:
10.3724/SP.J.1145.2015.03032
文献标志码:
A
摘要:
为揭示土壤初始pH值、温度和有机质含量三因素对多粘类芽孢杆菌(Paenibacillus polymyxa)L1-9菌株在黄瓜根际土壤定殖的影响,采用室内盆栽实验,在黄瓜一叶期灌根接种生防菌L1-9发酵液,设置土壤pH值、温度和有机质含量的不同处理,培养并定期采集黄瓜根际土壤样品,采用实时荧光定量PCR技术检测海洋生防菌L1-9在黄瓜根际土壤定殖的数量变化. 定量数据及其显著性检验结果表明:温度、初始pH值和有机质含量显著影响L1-9菌株在黄瓜根际土壤中的定殖. 土壤初始pH为6、7和8均可定殖. 初始pH值为7和8的处理,在多数调查时间内无显著差异,最大为每克干土(8.52 ±0.18) × 109 拷贝. 在存在显著性差异的时间段内,初始pH值为6时,L1-9菌株在黄瓜根际土壤中数量在同期中最大. 15-30 ℃培养时,最大数量为每克干土(2.13 ± 0.31) × 1010 拷贝. 温度越高,定殖越早,但在较高温度下定殖数量下降较快. 高有机质含量可以增加定殖数量,提高定殖能力,最大数量为每克干土(1.15 ± 0.04) × 1010 拷贝. 本研究表明实时荧光定量PCR技术适用于研究土壤根际生防菌的定殖;海洋生防菌L1-9能够在较宽温度、初始pH值和有机质含量范围内稳定定殖,较低温度、中性偏酸初始pH值和高有机质含量更有利于L1-9菌株在黄瓜根际土壤中定殖.
Abstract:
The objective of this paper was to reveal the effects of 3 soil environmental factors, including the initial pH value, the temperature and the organic matter contents, on Paenibacillus polymyxa L1-9 rhizosphere colonization. We monitored the changes of the L1-9 strain colonizing the rhizosphere soil of cucumber growing in pots using real-time fluorescence quantitative-PCR. At the one-leaf stage, cucumber was inoculated by the irrigation root method. And rhizosphere soil samples of cucumber were collected every 2 d, for a total of nine times. Then, we quantified the 16S rRNA gene number of the L1-9 strain in the cucumber rhizosphere, and researched the colonization characteristics and analyzed the effects of the environmental factors on L1-9 colonization in the rhizosphere soil of cucumber. The results showed that real-time PCR was suitable for colonization studies, and that the initial pH, temperature and organic matter contents all significantly influenced the colonization within a certain period. The biocontrol agent L1-9 could colonize soil with an initial pH of 6, 7 and 8. There were no significant differences between pH 7 and 8 treatments for the majority of investigations. Among all pH treatments in the same period, pH 6 had the greatest number of L1-9 strain, significantly different from others, which was (8.52 ± 0.18) × 109 copies per gram dry soil. Biocontrol agent L1-9 could maintain greater number over a longer period at 15°C than at higher temperatures. Colonization started earlier but also decreased more rapidly with higher temperatures. Among all the temperature treatments, the greatest 16S rRNA copies number of L1-9 strain was (2.13 ± 0.31) × 1010 per gram dry soil. On the other hand, high organic matter content increased L1-9 numbers and improved colonization ability, and the greatest 16S rRNA copies number of L1-9 strain was (1.15 ± 0.04) × 1010 per gram dry soil. The results indicated that Paenibacillus polymyxa L1-9 strain can colonize the rhizosphere soil of cucumber in varied conditions of initial pH, temperature and organic matter content. The condition of neutral to acidic pH, low temperature and high organic matter contents is favorable for L1-9 strain colonization in the cucumber rhizosphere soil.

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备注/Memo

备注/Memo:
江苏省省属高校自然科学重大基础研究项目(12KJA210001)和江苏省自然科学基金项目(BK20141248)资助 Supported by the Jiangsu Provincial University Natural Science Major Basic Research Project(12KJA210001) and the Natural Science Foundation of Jiangsu Province (BK20141248)
更新日期/Last Update: 2016-01-05