|本期目录/Table of Contents|

[1]徐小娜,崔浩天,陈亮宇,等.具有抗补体活性海洋放线菌的筛选及其活性物质的分离[J].应用与环境生物学报,2018,24(06):1295-1300.[doi:10.19675/j.cnki.1006-687x.2017.09038]
 XU Xiaona,et al..Screening of the marine actinomycetes with anti-complement activity and isolation of the active fractions[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1295-1300.[doi:10.19675/j.cnki.1006-687x.2017.09038]
点击复制

具有抗补体活性海洋放线菌的筛选及其活性物质的分离()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1295-1300
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Screening of the marine actinomycetes with anti-complement activity and isolation of the active fractions
作者:
徐小娜 崔浩天 陈亮宇 苏春 白凤武 赵心清
1大连理工大学生命科学与技术学院 大连 116023 2上海交通大学生命科学技术学院微生物代谢国家重点实验室 上海 200240 3 陕西师范大学生命科学学院 西安 710119
Author(s):
XU Xiaona et al.
1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China 2 State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China 3 College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China
关键词:
海洋放线菌抗补体活性次生代谢产物分离纯化
Keywords:
marine actinomycete anti-complement activity secondary metabolite isolation and purification
分类号:
Q939.91 : Q936
DOI:
10.19675/j.cnki.1006-687x.2017.09038
摘要:
抗补体活性物质研究对开发相关药物、治疗多种补体过度或非正常激活引起的疾病具有重要意义,然而目前微生物来源的抗补体活性物质研究还非常有限. 利用溶血法对从大连星海湾海泥样品中分离出来的42株海洋放线菌进行抗补体活性测试,并利用C18柱色谱层析及Sephadex LH-20凝胶柱层析,对具有较好抗补体活性的菌株DUT11的次生代谢产物进行分离纯化和结构鉴定. 结果显示:菌株 S187、M5、S088、M8、S063、DUT11 和 MD16 在TSB 培养基中发酵后发酵液提取物和菌丝体提取物均具有较好的抗补体活性,其中菌株 DUT11 的抗补体活性为最强. DUT11在M33培养基中发酵后所产生的次生代谢产物的抗补体活性最强,其发酵液和菌丝体提取物的活性分别为56.5% 和 60.8%. 进一步分离得到3个具有微弱抗补体活性的小分子化合物,分别鉴定为3-吲哚甲酸(1)、对羟基苯甲酸(2)和2-吡咯甲酸(3). 本研究表明在大连星海湾海泥样品中存在多种可产生抗补体活性物质的海洋链霉菌,产生的抗补体活性化合物活性较强,结果可为进一步开发利用海洋放线菌提供新的思路,为研究新型抗补体活性化合物奠定基础. (图3 表3 参26)
Abstract:
Studies on anti-complement agents are essential for the development of drugs to treat various diseases caused by excessive or abnormal activation of the complement system. However, studies on microbial-derived anti-complement agents are still very limited. The anti-complement activities of 42 marine-derived actinomycete strains isolated from the sediment samples collected from Xinghai Bay in Dalian were studied using the hemolysis method. In addition, the active fractions of the strain Streptomyces sp. DUT11 were separated by C18 and Sephadex LH-20 column chromatography, and the active compounds with anti-complement activity were identified. Streptomyces strains S187, M5, S088, M8, S063, DUT11 and MD16 showed good anti-complement activities upon being cultured in TSB medium, with the strain DUT11 showing the best anti-complement activity among these strains. When different fermentation media were tested, the highest anti-complement activity of DUT11 was observed in M33 fermentation medium. The anti-complement activity was 56.5% for the extract of supernatant and 60.8% for the extract of mycelia, respectively. Furthermore, three compounds with anti-complement activity were obtained from the active components, which were identified as 3-indolecarboxylic acid (1), p-hydroxy benzoic acid (2) and 2-pyrrolecarboxylic acid (3). These results demonstrated that marine-derived streptomycetes can be employed to produce active compounds with anti-complement activity. This study presents a new alternative for the utilization of marine actinomycetes and provides a basis for the exploration of new anti-complement agents.

参考文献/References:

1. Morgan, BP, Harris, CL. Complement, a target for therapy in inflammatory and degenerative diseases [J]. Nat Rev Drug, 2015, 14: 857-877
2. Katschke KJ Jr, Helmy KY, Steffek M, Xi H, Yin J, Lee WP, Gribling P, Barck KH, Carano RA, Taylor RE, Rangell L, Diehl L, Hass PE, Wiesmann C, van Lookeren Campagne M. A novel inhibitor of the alternative pathway of complement reverses inflammation and bone destruction in experimental arthritis [J]. J Exp Med, 2007, 204 (6): 1319-1325
3. Kolev MV, Ruseva MM, Harris CL, Morgan BP, Donev RM. Implication of complement system and its regulators in Alzheimer’s disease [J]. Curr Neuropharmacol, 2009, 7 (1): 1-8
4. Neher MD, Weckbach S, Flierl MA, HuberLang MS, Stahe PF. Molecular mechanisms of inflammation and tissue injury after major trauma-is complement the “bad guy”? [J]. J Biomed Sci, 2011, 18 (1): 90
5. Ricklin D, Lambris JD. Progress and trends in complement therapeutics [M]//Lambris JD, Holers VM, Ricklin D. Complement Therapeutics. New York: Springer, 2013: 1-22
6. Kamjam M, Sivalingam P, Deng Z, Hong K. Deep sea actinomycetes and their secondary metabolites [J]. Front Microbiol, 2017, 8 (760): 1-9
7. Managamuri U, Vijayalakshmi M, Ganduri VSRK, Rajulapati SB, Bonigala B, Kalyani BS. Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30. Biotechnology, 2017, 7 (3): 217
8. Yang CL, Wang YS, Liu CL, Zeng YJ, Cheng P, Jiao RH, Bao SX, Huang HQ, Tan RX, Ge HM. Strepchazolins A and B: two new alkaloids from a marine Streptomyces chartreusis NA02069 [J]. Mar Drugs, 2017, 15 (8): 1-8.
9. 耿香. 利用基因组挖掘发现两株海洋链霉菌的生物活性及活性物质初步研究[D]. 大连: 大连理工大学, 2014 [Geng X. Discovery of bioactivities of two marine-derived Streptomycetes by genome mining and preliminary studies of the bioactive agents [D]. Dalian: Dalian University of Technology, 2014]
10. 徐晗, 章蕴毅, 张建文, 陈道峰. 天然产物中的抗补体活性成分[J]. 中国天然药物, 2007, 5 (5): 322-332 [Xu H, Zhang YY, Zhang JW, Chen DF. Anti-complementary agents from natural products [J]. Chin J Nat Med, 2007, 5 (5): 322-332]
11. 谢予朋, 李阳, 孙晓迪, 孙世光. 中药活性成分抑制补体系统的研究进展[J]. 中国医药导报, 2013, 10 (33): 28-31 [Xie Y, Li Y, Sun XD, Sun SG. Advance research on the inhibition of active components of Chinese herbs to complement system [J]. Chin Med Her, 2013, 10 (33): 28-33]
12. Isao K, Katuo K, Shuji T. Complestatin, a potent anti-complement substance produced by Streptomyces lavendulae. I. Fermentation, isolation and biological characterization [J]. J Antibiot, 1989, 42 (2): 236-241
13. Singh SB, Jayasuriya H, Salituro GM, Zink DL, Shafiee A, Heimbuch B, Silverman KC, Lingham RB, Genilloud O, Teran A, Vilella D, Felock P, Hazuda D. The complestatins as HIV-1 integrase inhibitors. Efficient isolation, structure elucidation, and inhibitory activities of isocomplestatin, chloropeptin I, new complestatins, A and B, and acid-hydrolysis products of chloropeptin I [J]. J Nat Prod, 2001, 64 (7): 874-882
14. 徐小娜, 陈亮宇, 赵心清. 天然抗补体活性物质研究进展[J]. 天然产物研究与开发, 2015, 27 (2): 355-359 [Xu XN, Chen LY, Zhao XQ. Progress in the research and development of anti-complementary agents from natural products [J]. Nat Prod Ret Dev, 2015, 27 (2): 355-359]
15. Chen J, Wu Q, Hawas UW, Wang H. Genetic regulation and manipulation for natural product discovery [J]. Appl Microbiol Biotechnol, 2016, 100 (7): 2953-2965
16. Zhu H, Zhang Y, Zhang J, Chen D. Isolation and characterization of an anti-complementary protein-bound polysaccharide from the stem barks of Eucommia ulmoides [J]. Int Immunopharmacol, 2008, 8 (9): 1222-1230
17. 张学俊, 伊廷金, 孙黔云舢, 张文坤, 闰银萍, 龚桂珍, 宫本红. 杜仲叶多糖的提取分离、抗补体活性及结构研究[J]. 天然产物研究与开发, 2011, 23 (4): 606-611+637 [Zhang XJ, Yi TJ, Sun QY, Zhang WK, Yan YP, Gong GZ. Isolation, anti-complement activities, and chemical structures of polysaccharide from Eucommia ulmoides leaves [J]. Nat Prod Res Dev, 2011, 23 (4): 606-611+637]
18. Lee YK, Kim HW, Liu CL, Lee HK. A simple method for DNA extraction from marine bacteria that produce extracellular materials [J]. J Microbiol Meth, 2003, 52 (2): 245-250
19. 刘运. 美登木内生链霉菌Streptomyces sp.次生代谢产物研究[D]. 昆明: 云南大学, 2013 [Liu Y. The secondary metabolites research from Maytenus hookeri Streptomycetes sp. [D]. Kunming: Yunnan University of Technology, 2013]
20. 李文林, 毛士龙, 易杨华, 吕泰省, 许强芝, 汤海峰. 丰头皮海绵化学成分研究[J]. 中国海洋药物, 2000 (3): 1-4 [Li WL, Mao SL, Yi YG, Lü QS, Xu QZ, Tang HF. Studies on the chemical constitutions of the sponge Suberites tylobtusa Levi [J]. Chin J Mar Drugs, 2000 (3): 1-4]
21. 张健, 李友宾, 王大为, 殷志琦, 段金廒. 太子参化学成分研究[J]. 中国中药杂志, 2007, 32 (11): 1051-1053 [Zhang J, Li YB, Wang DW, Yin ZQ, Duan JO. Chemical constituents from roots Pseudostellaria heterophylla [J]. China J Chin Mater Med, 2007, 32 (11): 1051-1053]
22. Kaneko I, Kamoshida K, Takahashi S. Complestatin, a potent anti-complement substance produced by Streptomyces lavendulae. I. Fermentation, isolation and biological characterization [J]. J Antibiot, 1989, 42 (2): 236-241
23. Park OK, Choi HY, Kim GW, Kim WG. Generation of new complestatin analogs by heterologous expression of the complestatin biosynthetic gene cluster from Streptomyces chartreusis AN1542 [J]. ChemBioChem, 2016, 17 (18): 1725-1731
24. Kwon YJ, Kim HJ, Kim WG. Complestatin exerts antibacterial activity by the inhibition of fatty acid synthesis [J]. Biol Pharm Bull, 2015, 38 (5): 715-721
25. Singh SB, Jayasuriya H, Salituro GM, Zink DL, Shafiee A, Heimbuch B, Silverman KC, Lingham RB, Genilloud O, Teran A, Vilella D, Felock P, Hazuda D. The complestatins as HIV-1 integrase inhibitors. Efficient isolation, structure elucidation, and inhibitory activities of isocomplestatin, chloropeptin I, new complestatins, A and B, and acid-hydrolysis products of chloropeptin I [J]. J Nat Prod, 2001. 64 (7): 874-882
26. 王玉梅. 海洋链霉菌卤化酶基因筛选阳性菌株的活性物质研究[D]. 大连: 大连理工大学, 2014 [Wang YM. Studies on the halogenated substances from marine actinomycetes [D]. Dalian: Dalian University of Technology, 2014]
27.

更新日期/Last Update: 2018-12-25