|本期目录/Table of Contents|

[1]冯殿宝,王维红,王燕杉,等.以黏土为载体的好氧颗粒污泥培养及其对番茄废水的处理[J].应用与环境生物学报,2019,25(01):199-205.[doi:10.19675/j.cnki.1006-687x.2018.03033]
 FENG Dianbao,WANG Weihong**,WANG Yanshan & SU Kuizu.Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):199-205.[doi:10.19675/j.cnki.1006-687x.2018.03033]
点击复制

以黏土为载体的好氧颗粒污泥培养及其对番茄废水的处理()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
199-205
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater
作者:
冯殿宝 王维红 王燕杉 苏馈足
1新疆农业大学水利与土木工程学院 乌鲁木齐 830052 2合肥工业大学土木与水利工程学院 合肥 230009
Author(s):
FENG Dianbao1 WANG Weihong1** WANG Yanshan1 & SU Kuizu 2
1 College of Hydraulic and Civil Engineering, Xinjiang Agricultural University, Urumqi 830052, China 2 School of Civil Engineering, Hefei University of Technology, Hefei 230009, China
关键词:
番茄酱加工废水好氧颗粒污泥黏土高通量测序技术
Keywords:
tomato paste processing wastewater aerobic granular sludge clay high-throughput sequencing
分类号:
X703
DOI:
10.19675/j.cnki.1006-687x.2018.03033
摘要:
针对新疆番茄酱加工废水排放量大、有机物浓度高等问题,采用好氧颗粒污泥技术处理. 为了解黏土对于污泥好氧颗粒化进程的影响,在SBR反应器中接种絮体污泥并投加黏土,以人工合成番茄酱加工废水为基质培养好氧颗粒污泥,并采用扫描电镜、激光共聚焦(CLSM)、死活细菌染色以及高通量测序等技术表征活性污泥的颗粒化过程. 结果显示,在反应器中投加黏土运行20 d时获得平均粒径0.54 mm左右的好氧颗粒污泥. 颗粒污泥成熟后,污泥沉降性能较好,COD、NH4+-N、PO43--P的平均去除率分别达到90%、85%、40%以上. 扫描电镜下,颗粒污泥轮廓清晰,结构密实. CLSM显示,α-多糖、β-多糖和蛋白质作为胞外聚合物(EPS)的组成部分在颗粒中分布广泛,贯穿整个颗粒截面. 添加黏土培养的颗粒污泥死活细菌染色显示其死细菌数量积累程度更高,活细菌多位于外层并包裹着死细菌. 高通量测序结果显示,黏土投加后微生物多样性提高,Shannon指数由4.50增至4.79,动胶菌属和产黄菌属的相对丰度分别为37.87%和8.79%. 本研究表明投加黏土可促进好氧颗粒污泥形成,维持体系稳定运行及有机物降解效果主要源于微生物群落的共同作用. (图8 表1 参30)
Abstract:
The aerobic granular sludge technology was adopted to treat tomato-paste processing wastewater in Xinjiang because it receives high amount of discharge products and high concentration of organic matter. A sequencing batch reactor (SBR) was operated to investigate the effect of clay augmentation on aerobic granulation. Flocculent sludge and clay were fed into the synthetic tomato-paste processing wastewater contained in the SBR. The combined bio-analytical techniques of scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), live/dead staining, and high-throughput sequencing were used to characterize the granulation process of activated sludge. The results showed that the average particle size of the granules in the clay-added reactor was approximately 0.54 mm in 20 days. After maturation, the granular sludge had a good settling ability, and the average removal rate of COD, NH4+-N, and PO43--P reached 90%, 85%, and 40%, respectively. The SEM results showed that the granules had a clear outline and compacting structure. The CLSM results revealed that α-D-glucopyranose polysaccharide, β-D-glucopyranose polysaccharide, and protein, as components of extracellular polymeric substances (EPS), were widely distributed throughout the granule. The live/dead bacterial staining images of the clay-added granular sludge showed a high accumulation of dead bacteria. The live bacteria were distributed more closely towards the edge of the granule, while the dead bacteria were trapped inside. The high-throughput sequencing results showed that the addition of clay led to an increase in microbial diversity, with the Shannon index increasing from 4.50 to 4.79. The relative abundances of the aerobic bacterial genera Zoogloea and Flavobacterium were 37.87% and 8.79%, respectively. The study demonstrated that the addition of clay could accelerate the formation of aerobic granular sludge, and the maintenance of the stability of the system and degradation of organic matter mainly resulted from the combined action of microbial communities.

参考文献/References:

1 许述. 番茄废水污泥培养的优化研究[D]. 石河子: 石河子大学, 2013 [Xu S. Study of tomato waste water sludge culture optimization [D]. ShiHeZi: Shihezi University, 2013]
2 Moy BYP, Tay JH, Toh SK, Liu Y, Tay STL. High organic loading influences the physical characteristics of aerobic sludge granules [J]. Lett Appl Microbiol, 2002, 34 (6): 407-412
3 王建龙, 张子健, 吴伟伟. 好氧颗粒污泥的研究进展[J]. 环境科学学报, 2009, 29 (3): 449-473 [Wang JL, Zhang ZJ, Wu WW. Research advances in aerobic granular sludge [J]. Acta Sci Circum, 2009, 29 (3): 449-473]
4 Morgenroth E, Sherden T, Loosdrecht MCMV, Heijnen JJ, Wilderer PA. Aerobic granular sludge in a sequencing batch reactor [J]. Water Res, 1997, 31 (31): 3191-3194
5 Loosdrecht MCMV, Kreuk MKD. Formation of aerobic granules with domestic sewage [J]. J Environ Eng-ASCE, 2006, 132 (6): 694-697
6 Khan MZ, Khan F, Sabir S. Aerobic granular treatment of 2,4 dichloropheno l [J]. Can J Chem Eng, 2011, 89 (4): 914-920
7 刘名, 唐朝春, 衷诚, 陈惠民, 叶鑫. 干硝化好氧颗粒污泥对pb2+的吸附特性与机理[J]. 环境科学研究, 2015, 28 (12): 1923-1930 [Liu M, Tang CC, Zhong C, Cheng HM, Ye X. Dried biomass from nitrified aerobic granular sludge for pb2+ adsorption behaviors and mechanisms [J]. Res Environ Sci, 2015, 28 (12): 1923-1930]
8 Nancharaiah YV, Joshi HM, Mohan TVK, Venugopalan VP, Narasimhan SV. Aerobic granular biomass: a novel biomaterial for efficient uranium removal [J]. Curr Sci India, 2006, 91 (4): 503-509
9 Arrojo B, Mosqueracorral A, Garrido JM, Méndez R. Aerobic granulation with industrial wastewater in sequencing batch reactors [J]. Water Res, 2004, 38 (14-15): 3389-3399
10 Mata AMT, Ligneul A, Louren?o ND, Pinheiro HM. Advanced oxidation for aromatic amine mineralization after aerobic granular sludge treatment of an azo dye containing wastewater [J]. Desalin Water Treat, 2017, 91: 168-174
11 Thayalakumaran N, Bhamidimarri R, Bickers PO. Biological nutrient removal from meat processing wastewater using a sequencing batch reactor [J]. Water Sci Technol, 2003, 47 (10): 101-108
12 刘莉莉, 王志平, 蔡伟民. 好氧颗粒污泥处理啤酒废水的研究[J]. 工业用水与废水, 2006, 37 (4): 27-30 [Liu LL, Wang ZP, Cai WM. Treatment of brewery wastewater by aerobic granular sludge [J]. Ind Water Wastewater, 2006, 37 (4): 27-30]
13 Su KZ, Yu HQ. Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean-processing wastewater [J]. Environ Sci Technol, 2005, 39 (8): 2818-2827
14 Long B, Yang CZ, Pu WH, Yang JK, Jiang GS, Dan JF, Li CY, Liu FB. Rapid cultivation of aerobic granular sludge in a pilot scale sequencing batch reactor [J]. Bioresour Technol, 2014, 166 (8): 57-63
15 Wang S, Shi W, Yu S, Yi X. Rapid cultivation of aerobic granular sludge by bone glue augmentation and contaminant removal characteristics [J]. Water Sci Technol, 2013, 67 (7): 1627
16 信欣, 管蕾, 郭俊元, 刘洁, 冯梅, 余婷婷. SBR加载不同粒径磁性活性炭对其污泥颗粒化进程的影响机制[J]. 环境科学, 2017, 38 (11): 4679-4686 [Xin Z, Guan L, Guo JY, Liu J, Feng M, Yu TT. Effects of magnetic activated carbon with different particle sizes on sludge [J]. Environ Sci, 2017, 38 (11): 4679-4686]
17 汪皓东. 低温条件下好氧颗粒污泥快速培养及其稳定性研究[D]. 哈尔滨: 哈尔滨工业大学, 2010 [Wang HD. Stydy on rapid granulation and stability of aerobic granular sludge at low temperature [D]. Harbin: Harbin Institute of Technology, 2010]
18 Weber SD, Ludwig W, Schleifer KH, Fried J. Microbial composition and structure of aerobic granular sewage biofilms [J]. Appl Environ Microb, 2007, 73 (19): 6233
19 Zhu L, Qi HY, Lv ML, Kong Y, Yu YW, Xu XY. Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies [J]. Bioresour Technol, 2012, 124 (3): 455
20 国家环境保护总局. 水和废水监测分析方法[M]. 北京: 中国环境科学出版社, 2002 [State Enviromental Protection Adminitration of China. Method for Determination of Water and Wastwater. Beijing: China Environmental Science Press, 2002]
21 Yuan B, Wang X, Tang C, Li X, Yu G. In situ observation of the growth of biofouling layer in osmotic membrane bioreactors by multiple fluorescence labeling and confocal laser scanning microscopy [J]. Water Res, 2015, 75: 188
22 陈启伟, 苏馈足, 陈丁丁, 蔬童, 王维红. 处理番茄酱加工废水的活性污泥颗粒化过程[J]. 环境科学研究, 2018, 31 (2): 369-378 [Chen QW, Su KZ, Chen DD, Shu T, Wang WH. Process of aerobic granulation of activated sludge treating tomato paste processing wastewater [J]. Res Environ Sci, 2018, 31 (2): 369-378]
23 王香莲. 高氨氮(含酚)废水快速培养好氧颗粒污泥的试验研究[D]: 徐州: 中国矿业大学, 2016 [Wang XL. Experimental study on rapid cultivation of aerobic granules in high-strength ammonium wastewater with phenol [D]. Xuzhou: China University of Mining and Technology, 2016]
24 Chen MY, Lee DJ, Tay JH. Distribution of extracellular polymeric substances in aerobic granules [J]. Appl Microbial Biotechnol, 2007, 73 (6): 1463
25 高景峰, 张倩, 王金惠, 苏凯, 彭永臻. 颗粒活性炭对SBR反应器中好氧颗粒污泥培养的影响研究[J]. 应用基础与工程科学学报, 2012, 20 (3): 345-354 [Gao JF, Zhang Q, Wang JH, Su K, Peng YZ. Enhanced aerobic sludge granulation in sequencing batch reactor by granular activated carbon augmentation [J]. J Basic Sci Eng, 2012, 20 (3): 345-354]
26 Adav SS, Lee DJ, Tay JH. Extracellular polymeric substances and structural stability of aerobic granule [J]. Water Res, 2008, 42 (6-7): 1644
27 Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T. A human gut microbial gene catalogue established by metagenomic sequencing [J]. Nature, 2010, 464 (7285): 59-65
28 Weissbrodt DG, Neu TR, Ute K, Yoan R, Christof H. Assessment of bacterial and structural dynamics in aerobic granular biofilms [J]. Front Microbial, 2013, 4 (2): 175
29 Szabó E, Liébana R, Hermansson M, Modin O, Persson F, Wilén BM. Microbial population dynamics and ecosystem functions of anoxic/aerobic granular sludge in sequencing batch reactors operated at different organic loading rates [J]. Front Microbial, 2017, 8: doi.org/10.3389/fmicb.2017.00770
30 Jiang HL, Tay JH, Maszenan AM, Tay TL. Bacterial diversity and function of aerobic granules engineered in a sequencing batch reactor for phenol degradation [J]. Appl Environ Microb, 2004, 70 (11): 6767-6775

相似文献/References:

[1]李军,** 周延年 何梅 王亚宜 韦苏.城市污水处理厂好氧颗粒污泥的特性*[J].应用与环境生物学报,2008,14(05):640.
[2]陈国科,黄钧,毕京芳,等.好氧颗粒污泥耐受高碳氮负荷过程中的群体感应[J].应用与环境生物学报,2014,20(01):73.[doi:10.3724/SP.J.1145.2014.00073]
 CHEN Guoke,HUANG Jun,BI Jingfang,et al.Quorum sensing of aerobic granular sludge tolerating high carbon and nitrogen loads[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):73.[doi:10.3724/SP.J.1145.2014.00073]
[3]王硕,于水利,徐巧,等.好氧颗粒污泥特性、应用及形成机理研究进展[J].应用与环境生物学报,2014,20(04):732.[doi:10.3724/SP.J.1145.2014.01009]
 WANG Shuo,YU Shuili,XU Qiao,et al.Characteristics, application and formation mechanisms of aerobic granular sludge: recent advances[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):732.[doi:10.3724/SP.J.1145.2014.01009]
[4]关梦龙,黄钧,毕京芳,等.好氧颗粒污泥代谢高浓度有机废水的数学模拟[J].应用与环境生物学报,2014,20(06):1063.[doi:10.3724/SP.J.1145.2014.04005]
 GUAN Menglong,HUANG Jun,BI Jingfang,et al.Mathematical simulation of aerobic granular sludge metabolizing high-concentration organic wastewater[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):1063.[doi:10.3724/SP.J.1145.2014.04005]
[5]董晶晶,吴迪,马柯,等.好氧颗粒污泥工艺强化脱氮研究进展[J].应用与环境生物学报,2018,24(01):177.[doi:10.19675/j.cnki.1006-687x.2017.02008]
 DONG Jingjing,WU Di,MA Ke,et al.Review on enhanced denitrification of aerobic granular sludge technology[J].Chinese Journal of Applied & Environmental Biology,2018,24(01):177.[doi:10.19675/j.cnki.1006-687x.2017.02008]

更新日期/Last Update: 2019-02-25