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[1]孙娟,李东,郑涛,等.微量元素对蔬菜废弃物连续厌氧消化系统微生物群落结构的影响[J].应用与环境生物学报,2019,25(01):156-163.[doi:10.19675/j.cnki.1006-687x.2018.03035]
 SUN Juan,LI Dong**,ZHENG Tao**,et al.Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):156-163.[doi:10.19675/j.cnki.1006-687x.2018.03035]
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微量元素对蔬菜废弃物连续厌氧消化系统微生物群落结构的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
156-163
栏目:
研究论文
出版日期:
2019-03-01

文章信息/Info

Title:
Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste
作者:
孙娟 李东 郑涛 刘晓风 陈琳 何明阳
1常州大学城乡矿山研究院 常州 213100 2中国科学院成都生物研究所 成都 610041
Author(s):
SUN Juan1 LI Dong2** ZHENG Tao1** LIU Xiaofeng1 CHEN Lin1 & HE Mingyang1
1 Institute of Urban and Rural Mines, Changzhou University, Changzhou 213164, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
蔬菜废弃物厌氧消化微量元素微生物群落结构
Keywords:
vegetable waste anaerobic digestion trace element microbial community structure
分类号:
X705
DOI:
10.19675/j.cnki.1006-687x.2018.03035
摘要:
以蔬菜废弃物为原料的厌氧消化过程产甲烷能力下降时,通过添加微量元素可使其恢复稳定状态,因此研究微量元素对厌氧消化系统微生物结构的影响对优化系统性能具有重要意义. 采用70 L厌氧发酵罐,有效体积59.5 L,在中温35 ℃条件下进行蔬菜废弃物厌氧消化的连续冲击负荷试验,根据CH4含量变化规律,及时添加微量元素(Fe、Co、Ni)促进厌氧消化过程. 样品采用16S rRNA基因扩增和MiSeq测序技术分析微生物群落的结构. 结果表明,微量元素对细菌群落的影响主要作用于拟杆菌门、厚壁菌门及螺旋菌门. 在属水平上,第一次微量元素的添加诱导了拟杆菌门中的VadinBC27 wastewater-sludge的增加,相对丰度从54.1%升至68%,降低了厚壁菌门中Erysipelotrichaceae UGG-004以及螺旋菌门中Sphaerochaeta. 第二次微量元素的添加,主要降低了螺旋菌门中的Sphaerochaeta,相对丰度从11.4%到4.4%,以及诱导拟杆菌门中Bacteroides的产生,提高了原料利用率,降低了酸化的抑制作用. 微量元素对蔬菜废弃物厌氧消化过程中产甲烷菌群落的影响主要在甲基营养型Candidatus Methanoplasma、甲烷鬃菌属为主导的乙酸营养型. 当挥发性脂肪酸含量较高时,Candidatus Methanoplasma占主导地位,微量元素添加后则会诱导甲烷鬃菌为主导的乙酸营养型甲烷菌的产生,相对丰度从2.3%增至80%促进挥发性脂肪酸的消耗转化. 本研究表明,微量元素的添加对于微生物群落结构的改变显著,促进厌氧消化过程水解酸化与甲烷化的平衡,从而稳定运行. (图5 表2 参27)
Abstract:
To optimize the performance of continuous anaerobic digestion of vegetable waste, the relationship between trace elements and microbial community structure was explored. In this study, the continuous anaerobic digestion (AD) of vegetable waste (VW) was carried out in the 70-L anaerobic reactors under mesophilic conditions (35 ℃), and the trace element supplementation was based on the CH4 content in order to stabilize the process under high organic loading rate (OLR) for a long run-length. The microbial community structure was obtained through MiSeq sequencing of 16S rRNA gene amplicons. For the bacterial communities, the addition of trace elements had significant effects on Bacteroidetes, Spirochaetae, and Firmicutes. At the genus level, the first trace elements addition induced VadinBC27 wastewater-sludge to increase from 54.1% to 68% and reduced Erysipelotrichaceae UGG-004 and Sphaerochaeta. The first trace element addition induced the abundance of Bacteroides to increase, reduced that of Sphaerochaeta from 11.4% to 4.4% to improve the utilization of raw materials, and reduced the inhibition of acidification. For the methanogen communities, the addition of trace elements had significant effects on Candidatus Methanoplasma, which is a methantrophic-utilization methanogen and acetic-utilization methanogens with Methanosaeta dominated. Candidatus Methanoplasma dominated in the presence of high volatile fatty acids (VFA), and Methanosaeta dominated from 2.3% to 80% after the addition of trace elements to promote the consumption and conversion of VFA. Therefore, trace elements can promote the balance of hydrolysis and acidification and methanation in anaerobic digestion of vegetable waste, thus stabilizing the operation.

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更新日期/Last Update: 2019-02-25