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[1]刘鑫,侯若琳,任亚倩,等.寄生枣树的野生粗毛纤孔菌菌丝培养及其子实体大米栽培条件[J].应用与环境生物学报,2018,24(06):1283-1289.[doi:10.19675/j.cnki.1006-687x.2018.07045]
 LIU Xin,et al..Mycelial culture of wild Inonotus hispidus parasitizing the jujube tree and the cultivation conditions of its fruit body on rice medium[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1283-1289.[doi:10.19675/j.cnki.1006-687x.2018.07045]
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寄生枣树的野生粗毛纤孔菌菌丝培养及其子实体大米栽培条件()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1283-1289
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Mycelial culture of wild Inonotus hispidus parasitizing the jujube tree and the cultivation conditions of its fruit body on rice medium
作者:
刘鑫 侯若琳 任亚倩 金珊珊 陈磊 林文雄 郑明锋 傅俊生
1福建农林大学食品科学学院 福州 350002 2福建农林大学生命科学学院 福州 350002 3福建古田县食用菌研发中心,福建农林大学(古田)菌业研究院 古田 352200
Author(s):
LIU Xin et al.
1 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 Institute of Fungi Industry, Fujian Agriculture and Forestry University, Gutian 352200, China
关键词:
粗毛纤孔菌枣树培养条件大米液体菌种驯化栽培
Keywords:
Inonotus hispidus jujube tree culture condition rice liquid strain domestication cultivation
分类号:
Q949.329.81 : S567.3
DOI:
10.19675/j.cnki.1006-687x.2018.07045
摘要:
粗毛纤孔菌(Inonotus hispidus)是一种珍稀的药用真菌,野生资源非常匮乏,目前人工栽培粗毛纤孔菌技术尚未成熟,为了提高粗毛纤孔菌栽培生物转化率和缩短生产周期,首先对采集的野生粗毛纤孔菌菌丝培养条件进行分析,然后进一步探究采用大米栽培粗毛纤孔菌的方法. 在分析菌丝培养条件过程中,以菌丝长速为指标来筛选菌株菌丝生长适宜的温度、pH、碳源和氮源;在子实体栽培研究过程中,以生物转化率为指标来筛选适合菌株子实体生长的栽培培养基. 菌丝生长培养条件筛选结果表明,寄生枣树的野生粗毛纤孔菌菌丝生长的最适培养条件为温度25 ℃、初始pH 6.0、碳源葡萄糖、氮源酵母提取粉. 驯化栽培结果显示,粗毛纤孔菌在以大米为主要基质的培养基中生物转化率较高,优化后的最优栽培条件为营养液中酵母提取粉含量0.2%、大米与营养液的料液比1:1.6、液体菌种接种量4 mL,在该条件下,菌丝长满栽培培养基需4 d左右,原基分化形成子实体需20 d左右,生物转化率可达到28.70% ± 5.05%. 本研究结果表明以大米为主要基质栽培粗毛纤孔是可行的,同时也可为其他珍稀药用真菌寻找新的栽培基质提供思路. (图7 表6 参25)
Abstract:
Inonotus hispidus is a kind of rare medicinal fungus, and its natural resources are very scarce. Currently, the artificial cultivation technology of I. hispidus is not completely developed, and this reflects on its extremely low biological conversion rate and long cultivation period. In order to improve the bioconversion rate and shorten the production cycle of I. hispidus, we first analyzed the mycelia culture conditions of the collected I. hispidus, and then we further explore the method of domesticated cultivation of its fruiting body in rice medium. During the process of mycelial culture, the suitable temperature, pH, carbon source, and nitrogen source for mycelial growth were selected using the mycelial growth rate as index. During the domesticated cultivation of the fruiting body, the suitable culture medium for its growth was selected using the bioconversion rate as index. Screening results of mycelial culture conditions showed that the optimal culture conditions for the growth of mycelium of the wild I. hispidus were: temperature of 25 ℃, initial pH of 6.0, glucose as the carbon source, and yeast extract powder as the source of nitrogen. The results of the domesticated cultivation showed that the biotransformation rate of I. hispidus was higher when using rice as the main medium substrate. The optimal cultivation conditions were: a 0.2% yeast extract content in the nutrient solution, a 1:1.6 ratio of rice to nutrient solution, and a 4 mL inoculum of the liquid strain. Under these conditions, it took about 4 days for the mycelium to grow over the cultivation medium. The time required for the differentiation of the primordium to form fruit bodies was about 20 days, and the bioconversion rate reached 28.70% ± 5.05%. The results of this study indicate the feasibility of using rice as the main substrate for the cultivation of I. hispidus, and it also provide new insights for the finding of new cultivation substrates for other rare medicinal fungi.

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更新日期/Last Update: 2018-12-25