|本期目录/Table of Contents|

[1]史艳霞,张永军,王桂荣,等.Bt抗性和敏感棉铃虫幼虫中肠主要蛋白酶活性的变化[J].应用与环境生物学报,2008,14(03):394-398.
 SHI Yanxia,et al..Changes in Mid-gut Protease Activities in Larvae of Bt Resistant and Susceptible Strains of Helicoverpa armigera Hübner[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):394-398.
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Bt抗性和敏感棉铃虫幼虫中肠主要蛋白酶活性的变化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年03期
页码:
394-398
栏目:
综述
出版日期:
2008-03-25

文章信息/Info

Title:
Changes in Mid-gut Protease Activities in Larvae of Bt Resistant and Susceptible Strains of Helicoverpa armigera Hübner
作者:
史艳霞张永军王桂荣梁革梅高继国吴孔明郭予元
Author(s):
SHI Yanxia et al.
(1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China) (2College of Life Sciences, Northeast Agriculture University, Harbin 150030, China)
关键词:
关键词 棉铃虫 Bt抗性酶活性 pH值
Keywords:
Keywords Helicoverpa armigera Bt resistance enzyme activity pH value
Abstract:
Abstract The optimum pH value of mid-gut protease in larvae of both Bt resistant and susceptible strains of Helicoverpa armigera Hübner was determined in laboratory. The results showed that the optimum pH values of total protease in both two strains were 10.5. The optimum pH values of active alkaline trypsin-like enzyme in the two strains were 10.5 except that of the third instar larvae of resistant H. armigera was 8.0, while the optimum pH value of weak alkaline trypsin-like enzyme in the two strains was 8.0. However, the optimum pH values of chymotrypsin-like enzyme in the two strains were 8.50 except that of the fifth instar larvae of resistant H. armigera was 8.0. The activities of mid-gut protease in both resistant and susceptible strains under the optimum pH condition were also compared. There was no significant difference in the activities of total mid-gut protease of the two strains. It was shown that the activities of active alkaline trypsin-like enzyme in the fifth instar larvae of the two strains were not significantly different, while those of the third instar larvae of the two strains were significantly different. We also found that there was no difference in the weak alkaline trypsin-like enzyme activities between the third instar of both two strains, and those of the fifth instar larvae of the two strains were significantly different. As for chymotrypsin-like enzyme, it was found that there was a significant difference in the activities of the two strains. It was concluded that the changes in pH values and mid-gut protease activities could bring out resistance evolution of H. armigera larvae to Bt insecticidal proteins. Fig 2, Tab 1, Ref 17

相似文献/References:

[1]魏伟,钱迎倩,马克平.害虫对转基因Bt作物的抗性及其管理对策[J].应用与环境生物学报,1999,5(02):215.
 WEI Wei,et al..Pests resistance to transgenic Bt crops and their management strategies[J].Chinese Journal of Applied & Environmental Biology,1999,5(03):215.
[2]王桂荣,徐广,谭维嘉,等.菊酯类杀虫剂抗性棉铃虫para型钠离子通道基因的部分序列测定与突变[J].应用与环境生物学报,2004,10(03):332.
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[3]黄小红,陈清西,王君,等.Zn2+对棉铃虫N-乙酰-β-D-氨基葡萄糖苷酶活力与性质的影响[J].应用与环境生物学报,2005,11(06):711.
 HUANG Xiaohong,et al..Effect of Zn2 + on activity and p roperties of β-N-Acetyl-D-glucosaminidase from Helicoverpa arm igera pupae[J].Chinese Journal of Applied & Environmental Biology,2005,11(03):711.

更新日期/Last Update: 2008-07-01