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[1]侯若琳,刘鑫,王丹亭,等.一株桃树内生真菌Pestalotiopsis vismiae N1培养条件优化与抗氧化、抗菌活性分析[J].应用与环境生物学报,2019,25(02):385-391.[doi:10.19675/j.cnki.1006-687x.2018.05026]
 HOU Ruolin,LIU Xin,WANG Danting,et al.Optimization of culture conditions, and analysis of the antioxidant and antibacterial activities of the endophyte Pestalotiopsis vismiae N1[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):385-391.[doi:10.19675/j.cnki.1006-687x.2018.05026]
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一株桃树内生真菌Pestalotiopsis vismiae N1培养条件优化与抗氧化、抗菌活性分析
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
385-391
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Optimization of culture conditions, and analysis of the antioxidant and antibacterial activities of the endophyte Pestalotiopsis vismiae N1
作者:
侯若琳刘鑫王丹亭陈磊林文雄郑明锋傅俊生
1福建农林大学食品科学学院 福州 350002 2福建农林大学生命科学学院 福州 350002
Author(s):
HOU Ruolin1 LIU Xin1 WANG Danting2 CHEN Lei2 LIN Wenxiong2 ZHENG Mingfeng1** & FU Junsheng2**
1 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
韦司梅拟盘多毛孢生长培养抗氧化活性抗菌活性
Keywords:
Pestalotiopsis vismiae culture condition antioxidant activity antibacterial activity
分类号:
Q939.9
DOI:
10.19675/j.cnki.1006-687x.2018.05026
摘要:
对一株内生真菌韦司梅拟盘多毛孢(Pestalotiopsis vismiae)的培养条件进行探究,在此基础上进一步对其发酵提取物抗氧化、抗菌活性进行分析. 通过测定菌丝的生长速度寻找菌丝培养适宜的温度、碳源、氮源和pH,并采用正交试验优化培养条件;通过测定菌株深层发酵液提取物对羟基和DPPH自由基的清除率以分析其抗氧化活性;通过测定菌株深层发酵液提取物对多种病原细菌抑菌圈大小的影响来分析其抗菌活性. 结果表明,韦司梅拟盘多毛孢菌丝最适宜培养温度为25 ℃,在该温度培养条件下菌丝生长最佳碳源为蔗糖,最佳氮源为酵母提取粉,最佳初始pH为6.5,这3种因素的显著性差异大小顺序为碳源 > 氮源 > pH;菌株深层发酵液提取物具有良好的抗氧化活性,其对羟基自由基和DPPH自由基清除率的EC50分别为1.19 mg/mL和0.56 mg/mL;菌株发酵液提取物对铜绿假单胞杆菌、金黄色葡萄球菌均具有一定的抑制作用;深层发酵液提取物抗氧化和抗菌活性具有一定的稳定性. 本研究结果可为深入开发利用该真菌资源提供科学依据. (图12 表3 参26)
Abstract:
In this study, the culture conditions of endophytic fungus Pestalotiopsis vismiae were investigated, and the antioxidant and antibacterial activities were analyzed to provide a basis for further development and utilization of P. vismiae. The mycelial growth rate was determined to explore the suitable temperature, carbon source, nitrogen source, and pH for mycelial growth, and the orthogonal test was performed to optimize the culture conditions. The antioxidant activity of the extract from the deep fermentation broth was analyzed by measuring the hydroxyl radical and DPPH radical scavenging rates. The antibacterial activity of the extract from the deep fermentation broth was studied by measuring the size of the inhibition zone for various pathogenic bacteria. The results showed that the optimum growth temperature for P. vismiae was 25 ℃, the best carbon source for mycelial growth at 25 ℃ was sucrose, the optimum nitrogen source was yeast extract, and the optimum initial pH was 6.5. The order of the significant differences among the three factors was carbon source > nitrogen source > pH. The submerged fermentation broth extract of P. vismiae had good antioxidant activity, and the EC50 of the hydroxyl radical and DPPH radical scavenging rates was 1.19 mg/mL and 0.56 mg/mL, respectively. The strain fermentation broth extract had certain inhibitory effects on Pseudomonas aeruginosa and Staphylococcus aureus. The antioxidant and antibacterial activity of P. vismiae had a certain stability. The results of this study provided a scientific basis for the development and utilization of these fungal resources.

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更新日期/Last Update: 2019-04-25