|本期目录/Table of Contents|

[1]徐少慧,蒋代华,史鼎鼎,等.重金属复合污染土壤中耐铅镉微生物的筛选及鉴定[J].应用与环境生物学报,2019,25(03):532-538.[doi:10.19675/j.cnki.1006-687x.201808028]
 XU Shaohui,JIANG Daihua,**,et al.Screening and identification of lead and cadmium resistant microorganisms from combined heavy metal pollution soil[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):532-538.[doi:10.19675/j.cnki.1006-687x.201808028]
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重金属复合污染土壤中耐铅镉微生物的筛选及鉴定
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
532-538
栏目:
重金属污染及生物修复专栏
出版日期:
2019-06-25

文章信息/Info

Title:
Screening and identification of lead and cadmium resistant microorganisms from combined heavy metal pollution soil
作者:
徐少慧蒋代华史鼎鼎张蓉蓉黄智刚杨钙仁
1广西大学农学院 南宁 530004 2广西农业环境与农产品安全重点实验室培养基地 南宁 530004 3广西大学林学院 南宁 530004
Author(s):
XU Shaohui1 JIANG Daihua1 2** SHI Dingding1 ZHANG Rongrong1 HUANG Zhigang1 2 & YANG Gairen3
1 Agricultural College, Guangxi University, Nanning 530004, China 2 Guangxi Key Laboratory of Agricultural Environment and Agricultural Product Safety Cultivation Base, Nanning 530004, China 1 Forest College of Guangxi University, Nanning 530004, China
关键词:
重金属污染耐铅镉微生物筛选鉴定
Keywords:
heavy metal pollution lead and cadmium resistant microorganism screening identification
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.201808028
摘要:
为获得复合重金属高耐性微生物资源菌株,采集广西桂西北尾矿区重金属污染的土壤样品,采用改良的马丁氏培养基分离和筛选既耐铅又耐镉的高耐性微生物. 结果获得了一株耐铅浓度为1 200 mg/L、镉浓度为120 mg/L的铅镉复合耐性真菌——菌株K-3. 该菌株(K-3)最适生长环境为温度30 ℃、pH值7.0、盐浓度3%. 通过形态结合分子生物学鉴定得知,K-3菌株与已知分类地位的标准菌株Uncultured Westerdykella的18S rDNA 序列一致性为99%. 本研究获得的铅镉复合耐性真菌——菌株K-3是重金属铅和镉的最强双耐真菌之一,可为微生物或微生物联合植物修复重金属污染土壤提供重要的微生物种质资源. (图8 表3 参39)
Abstract:
To obtain the microbial resource strains with high heavy metal compound tolerance to remedy heavy mental contaminated soil, soil samples were collected from the tail mining area in Northwest Guangxi. Improved Martin’s medium was used to isolate and screen the high-tolerance microorganisms for heavy metal lead and cadmium. The fungus with compound tolerance to lead and cadmium termed K-3 was obtained, whose lead resistance was 1 200 mg/L and cadmium resistance was 120 mg/L. The optimum temperature for K-3 was 30 ℃, pH was 7.0, and salt concentration was 3%. The identification of its morphology and molecular biology showed that the sequence consistency of 18S rDNA between K-3 and the standard strain Uncultured Westerdykella was 99%. By consulting relevant literature and comprehensive inference, it was shown that K-3 was one of the strongest double-tolerance fungi for heavy metal lead and cadmium. It provided precious microbial germplasm resources for remediation of heavy metal contaminated soil using microorganisms or microorganism-associated plants.

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更新日期/Last Update: 2019-06-25